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Mol Cell Biol
1994 Mar 01;143:1893-900. doi: 10.1128/mcb.14.3.1893-1900.1994.
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The deadenylation conferred by the 3' untranslated region of a developmentally controlled mRNA in Xenopus embryos is switched to polyadenylation by deletion of a short sequence element.
Bouvet P
,
Omilli F
,
Arlot-Bonnemains Y
,
Legagneux V
,
Roghi C
,
Bassez T
,
Osborne HB
.
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The maternal Xenopus Eg mRNAs are adenylated and translated in the mature oocyte and then, after fertilization, are deadenylated and released from polysomes. Therefore, after fertilization, a change occurs in the cellular mechanisms that control mRNA adenylation. In the study reported here, we show that the 3' untranslated region of Eg2 mRNA contains a cis-acting element that is required for the deadenylation of chimeric RNAs after fertilization. This cis-acting element is contained within a single 17-nucleotide portion of the Eg2 mRNA. Disruption of this deadenylation element allows adenylation of the chimeric transcripts in the embryo. Therefore, this cis-acting element is part of the sequence information required for the developmental switch from adenylation to deadenylation of the maternal Eg2 mRNA in Xenopus embryos.
Bachvarova,
A maternal tail of poly(A): the long and the short of it.
1992, Pubmed
Bachvarova,
A maternal tail of poly(A): the long and the short of it.
1992,
Pubmed
Brewer,
Poly(A) shortening and degradation of the 3' A+U-rich sequences of human c-myc mRNA in a cell-free system.
1988,
Pubmed
Duval,
Stability of maternal mRNA in Xenopus embryos: role of transcription and translation.
1990,
Pubmed
,
Xenbase
Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase
Fox,
Poly(A) removal during oocyte maturation: a default reaction selectively prevented by specific sequences in the 3' UTR of certain maternal mRNAs.
1990,
Pubmed
,
Xenbase
Galili,
Role of the 3'-poly(A) sequence in translational regulation of mRNAs in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Harland,
Stability of RNA in developing Xenopus embryos and identification of a destabilizing sequence in TFIIIA messenger RNA.
1988,
Pubmed
,
Xenbase
Huarte,
Transient translational silencing by reversible mRNA deadenylation.
1992,
Pubmed
Hyman,
Translational inactivation of ribosomal protein mRNAs during Xenopus oocyte maturation.
1988,
Pubmed
,
Xenbase
Jackson,
Do the poly(A) tail and 3' untranslated region control mRNA translation?
1990,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Kuge,
Maturation of Xenopus laevis oocyte by progesterone requires poly(A) tail elongation of mRNA.
1992,
Pubmed
,
Xenbase
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Laird-Offringa,
Poly(A) tail shortening is the translation-dependent step in c-myc mRNA degradation.
1990,
Pubmed
Le Guellec,
Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein.
1991,
Pubmed
,
Xenbase
Legagneux,
Identification of RNA-binding proteins specific to Xenopus Eg maternal mRNAs: association with the portion of Eg2 mRNA that promotes deadenylation in embryos.
1992,
Pubmed
,
Xenbase
McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase
McGrew,
Translational control by cytoplasmic polyadenylation during Xenopus oocyte maturation: characterization of cis and trans elements and regulation by cyclin/MPF.
1990,
Pubmed
,
Xenbase
Munroe,
Tales of poly(A): a review.
1990,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Osborne,
Expression and post-transcriptional regulation of ornithine decarboxylase during early Xenopus development.
1991,
Pubmed
,
Xenbase
Paris,
Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2.
1991,
Pubmed
,
Xenbase
Paris,
Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development.
1990,
Pubmed
,
Xenbase
Paris,
Changes in the polyadenylation of specific stable RNA during the early development of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Peppel,
Deadenylation and turnover of interferon-beta mRNA.
1991,
Pubmed
Simon,
Translational control by poly(A) elongation during Xenopus development: differential repression and enhancement by a novel cytoplasmic polyadenylation element.
1992,
Pubmed
,
Xenbase
Stoeckle,
High-resolution analysis of gro alpha mRNA poly(A) shortening: regulation by interleukin-1 beta.
1993,
Pubmed
Varnum,
Deadenylation of maternal mRNAs during Xenopus oocyte maturation does not require specific cis-sequences: a default mechanism for translational control.
1990,
Pubmed
,
Xenbase
Vassalli,
Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.
1989,
Pubmed
Wahle,
The end of the message: 3'-end processing leading to polyadenylated messenger RNA.
1992,
Pubmed
Wharton,
RNA regulatory elements mediate control of Drosophila body pattern by the posterior morphogen nanos.
1991,
Pubmed
Wickens,
In the beginning is the end: regulation of poly(A) addition and removal during early development.
1990,
Pubmed
Wilson,
Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3' AU-rich sequences.
1988,
Pubmed
