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Abstract
A monoclonal antibody 2F7.C7 which identifies an epidermal antigen common to both anuran and urodele amphibia is described. This antigen has a high molecular weight and is expressed at stage 12 1/2 first inside and then on the surface of epidermal cells. It is not expressed on presumptive nervous tissue. It is expressed on all cells of the larval epidermis including ciliated and nonciliated cells and sucker. The relationship of the epidermal antigen to 'epimucin' is investigated by blocking experiments using Peanut agglutinin. These studies suggest that either the molecule recognized by 2F7.C7 is not epimucin, or that its epitope is far away from the lectin-binding site. Studies using this antibody in grafting and sandwich experiments are discussed and an overall scheme of epidermal development in Xenopus is presented.
Fig. 1 Developmental expression of 2F7.C7 onX. borealis embryos Cryostat sections of
different stages of development stained with 2F7.C7 and FITC conjugated Rabbit anti
mouse IgG. (A) Stage-11 gastrula (B/C) High-power stage-11 gastrula (D) Stage 122 (E)
Stage 15 neurula (F) Stage 19 neurula. Bar = 180[im (A, D, E, F); bar = 75 pm (B, C)
Fig. 2 Regional distribution of 2F7.C7 on X. borealis, X. laevis and A. mexicanum
High-power phase and fluorescence of cryostat sections from neurulae stained with
2F7.C7 and FITC-conjugated rabbit antimouse IgG. (A, B) Stage-122 X. borealis
epidermis (C, D) Stage-15 X. borealis with developing neural tube (arrow) (E, F) Stage-
19 X. borealis with complete neural tube (arrow) (G) Stage-19 A. mexicanum epidermis
(H) Stage-19 X. laevis epidermis. Bar = 75 \im
Fig. 3 Molecular identification of the epidermal marker defined by 2F7.C7 Immunoprecipitation
of iodogen-labelled ectodermal extract with 2F7.C7 run on 15 % SDS
PAGE and autoradiographed. (a) V8 protease treated 2F7.C7 immunoprecipitate (b)
2F7.C7 immunoprecipitate (c) NSI immunoprecipitate (d) Total labelled ectodermal
extract
Fig. 4 Blocking of 2F7.C7 with Peanut Agglutinin Cryostat sections of Stage-125 (A, B,
C, D) or stage-19 (E, F, G, H) X. borealis embryos. (A, E) Stained with FITCconjugated
PNA (B, F) Blocked with 20 \xg PNA and stained with FITC-conjugated
PNA (C, G) Stained with 2F7.C7 (D, H) Blocked with 20 ^g PNA and stained with
2F7.C7. Bar = 75 urn
Fig. 5 Scheme or epidermal differentiation in Xenopus
