XB-ART-27478
Dev Biol
1988 Jun 01;1272:452-5.
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Development of acetylcholinesterase induced by basic polypeptide-coated latex beads in cultured Xenopus muscle cells.
Abstract
The formation of acetylcholine receptor (AChR) clusters can be induced by basic polypeptide-coated latex beads in cultured Xenopus muscle cells. Here we investigated the development of acetylcholinesterase (AChE) at the bead-induced AChR clusters. AChE activity began to appear at the clusters after 1 day of bead-muscle coculture and was present at all of the bead-induced clusters within 4-7 days. Electron microscopy revealed that AChE reaction products were discretely localized within the cleft and the membrane invaginations at the bead-muscle contacts. Thus, the beads can mimic the nerve in inducing a local accumulation of both the AChRs and AChE, suggesting that the development of both specializations can be effected by a common stimulus.
PubMed ID: 3378675
Article link: Dev Biol
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Species referenced: Xenopus laevis
Genes referenced: ache