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J Am Soc Mass Spectrom
2001 Aug 01;128:877-84. doi: 10.1016/S1044-0305(01)00267-7.
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Targeted use of exoglycosidase digestion for the structural elucidation of neutral O-linked oligosaccharides.
Xie Y
,
Tseng K
,
Lebrilla CB
,
Hedrick JL
.
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Exoglycosidase digestion in combination with the catalog-library approach (CLA) is used with matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) to obtain the complete structure of oligosaccharides. The CLA is a collision-induced dissociation (CID)-based method used to determine the structure of O-linked neutral oligosaccharides. It provides both linkage and stereochemical information. Exoglycosidases are used to confirm independently the validity of the CLA. In some cases, the CLA provides structural information on all but a single residue. Exoglycosidase is used to refine these structures. In this way, exoglycosidase use is targeted employing only a small number of enzymes. Exoglycosidase arrays, which have been used with N-linked oligosaccharides, is avoided despite the larger variations in structures of O-linked species.
Burlingame,
Mass spectrometry.
1994,
Pubmed
Cancilla,
Alkaline degradation of oligosaccharides coupled with matrix-assisted laser desorption/ionization Fourier transform mass spectrometry: a method for sequencing oligosaccharides.
1998,
Pubmed
Colangelo,
On-target exoglycosidase digestions/MALDI-MS for determining the primary structures of carbohydrate chains.
1999,
Pubmed
Desaire,
Multicomponent quantification of diastereomeric hexosamine monosaccharides using ion trap tandem mass spectrometry.
1999,
Pubmed
Geyer,
Structural analysis of glycoconjugates by on-target enzymatic digestion and MALDI-TOF-MS.
1999,
Pubmed
Hedrick,
Structure and function of the extracellular matrix of anuran eggs.
1991,
Pubmed
,
Xenbase
König,
Evidence for linkage position determination in cobalt coordinated pentasaccharides using ion trap mass spectrometry.
1998,
Pubmed
Mechref,
Mass spectrometric mapping and sequencing of N-linked oligosaccharides derived from submicrogram amounts of glycoproteins.
1998,
Pubmed
Penn,
Collision-induced dissociation of branched oligosaccharide ions with analysis and calculation of relative dissociation thresholds.
1996,
Pubmed
Phillips,
Structural studies of the lipooligosaccharides from Haemophilus influenzae type b strain A2.
1993,
Pubmed
Quill,
The fertilization layer mediated block to polyspermy in Xenopus laevis: isolation of the cortical granule lectin ligand.
1996,
Pubmed
,
Xenbase
Reinhold,
Carbohydrate molecular weight profiling, sequence, linkage, and branching data: ES-MS and CID.
1995,
Pubmed
Strecker,
Primary structure of 12 neutral oligosaccharide-alditols released from the jelly coats of the anuran Xenopus laevis by reductive beta-elimination.
1995,
Pubmed
,
Xenbase
Tseng,
Catalog-library approach for the rapid and sensitive structural elucidation of oligosaccharides.
1999,
Pubmed
Tseng,
Characterization of neutral oligosaccharide-alditols from Xenopus laevis egg jelly coats by matrix-assisted laser desorption Fourier transform mass spectrometry.
1997,
Pubmed
,
Xenbase
Varki,
Biological roles of oligosaccharides: all of the theories are correct.
1993,
Pubmed
Wong,
Selection of anionic dopant for quantifying desialylation reactions with MALDI-FTMS.
2000,
Pubmed
Wong,
Anion dopant for oligosaccharides in matrix-assisted laser desorption/ionization mass spectrometry.
1999,
Pubmed
Yang,
Simplifying the exoglycosidase digestion/MALDI-MS procedures for sequencing N-linked carbohydrate side chains.
1996,
Pubmed
Yoon,
Linkage position determination in a novel set of permethylated neutral trisaccharides by collisional-induced dissociation and tandem mass spectrometry.
1992,
Pubmed
Zhao,
Rapid, sensitive structure analysis of oligosaccharides.
1997,
Pubmed
