Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-21663
J Biol Chem 1994 Jan 28;2694:2632-6.
Show Gene links Show Anatomy links

Cloning and expression of a cDNA for the human prostanoid FP receptor.

Abramovitz M , Boie Y , Nguyen T , Rushmore TH , Bayne MA , Metters KM , Slipetz DM , Grygorczyk R .


???displayArticle.abstract???
A cDNA clone coding for a functional human prostanoid FP receptor has been isolated from a uterus cDNA library. The human FP receptor consists of 359 amino acid residues with a predicted molecular mass of 40,060, and has the seven putative transmembrane domains characteristic of G-protein-coupled receptors. Challenge of Xenopus oocytes expressing the FP receptor with 10 nM of either prostaglandin (PG) F2 alpha or the selective FP-receptor agonist fluprostenol resulted in an elevation in intracellular Ca2+. Radioreceptor binding studies using membranes prepared from mammalian COS cells transfected with the FP receptor cDNA showed that the rank order of potency for prostaglandins and prostaglandin analogs in competition for [3H]PGF2 alpha specific binding sites was as predicted for the FP receptor, with PGF2 alpha approximately fluprostenol > PGD2 > PGE2 > U46619 > iloprost. In summary, we have cloned the human prostanoid FP receptor which is functionally coupled to the Ca2+ signalling pathway.

???displayArticle.pubmedLink??? 8300593
???displayArticle.link??? J Biol Chem


Species referenced: Xenopus laevis
Genes referenced: ptgds