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In the intestine during amphibian metamorphosis, stem cells appear, actively proliferate, and differentiate into an adult epithelium analogous to the mammalian counterpart. To clarify the molecular mechanisms regulating this process, we focused on a bZip-containing transcription factor (TH/bZip). We previously isolated TH/bZip from the Xenopus intestine as one of the candidate genes involved in adult epithelial development. Northern blot and in situ hybridization analyses showed that the transient and region-dependent expression of TH/bZip mRNA correlates well with the growth of adult epithelial primordia originating from the stem cells throughout the Xenopus intestine. To investigate its role in the adult epithelial development, we established an in vitro gene transfer system by using electroporation and organ culture techniques, and we overexpressed TH/bZip in the epithelium of Xenopus tadpole intestines. In the presence of thyroid hormone (TH) where the adult epithelial primordia appeared after 3 days of cultivation, overexpression of TH/bZip significantly increased their proliferating activity. On the other hand, in the absence of TH where the epithelium remained as larval-type without any metamorphic changes, ectopic expression of TH/bZip significantly increased the proliferating activity of the larval epithelium but had no effects on its differentiated state. These results indicate that TH/bZip functions as a growth activator during amphibian intestinal remodeling, although TH/bZip expression in the epithelium alone is not sufficient for inducing the stem cells.
Amano,
Isolation of genes involved in intestinal remodeling during anuran metamorphosis.
1998, Pubmed,
Xenbase
Amano,
Isolation of genes involved in intestinal remodeling during anuran metamorphosis.
1998,
Pubmed
,
Xenbase
Berry,
The expression pattern of thyroid hormone response genes in remodeling tadpole tissues defines distinct growth and resorption gene expression programs.
1998,
Pubmed
,
Xenbase
Berry,
The expression pattern of thyroid hormone response genes in the tadpole tail identifies multiple resorption programs.
1998,
Pubmed
,
Xenbase
Booth,
Gut instincts: thoughts on intestinal epithelial stem cells.
2000,
Pubmed
Brown,
The thyroid hormone-induced tail resorption program during Xenopus laevis metamorphosis.
1996,
Pubmed
,
Xenbase
Buchholz,
A dominant-negative thyroid hormone receptor blocks amphibian metamorphosis by retaining corepressors at target genes.
2003,
Pubmed
,
Xenbase
Buckbinder,
Thyroid hormone-induced gene expression changes in the developing frog limb.
1992,
Pubmed
,
Xenbase
Cowell,
Transcriptional repression by a novel member of the bZIP family of transcription factors.
1992,
Pubmed
Cowell,
Transcriptional repression by the human bZIP factor E4BP4: definition of a minimal repression domain.
1994,
Pubmed
Damjanovski,
Function of thyroid hormone receptors during amphibian development.
2002,
Pubmed
,
Xenbase
Denver,
Thyroid hormone-dependent gene expression program for Xenopus neural development.
1997,
Pubmed
,
Xenbase
Fu,
Novel double promoter approach for identification of transgenic animals: A tool for in vivo analysis of gene function and development of gene-based therapies.
2002,
Pubmed
,
Xenbase
Furlow,
In vitro and in vivo analysis of the regulation of a transcription factor gene by thyroid hormone during Xenopus laevis metamorphosis.
1999,
Pubmed
,
Xenbase
Ishizuya-Oka,
Temporal and spatial regulation of a putative transcriptional repressor implicates it as playing a role in thyroid hormone-dependent organ transformation.
1997,
Pubmed
,
Xenbase
Ishizuya-Oka,
Anteroposterior gradient of epithelial transformation during amphibian intestinal remodeling: immunohistochemical detection of intestinal fatty acid-binding protein.
1997,
Pubmed
,
Xenbase
Ishizuya-Oka,
Thyroid hormone-upregulated expression of Musashi-1 is specific for progenitor cells of the adult epithelium during amphibian gastrointestinal remodeling.
2003,
Pubmed
,
Xenbase
Ishizuya-Oka,
Cell-specific and spatio-temporal expression of intestinal fatty acid-binding protein gene during amphibian metamorphosis.
1994,
Pubmed
,
Xenbase
Ishizuya-Oka,
Induction of metamorphosis by thyroid hormone in anuran small intestine cultured organotypically in vitro.
1991,
Pubmed
,
Xenbase
Ishizuya-Oka,
Thyroid hormone-induced apoptosis of larval cells and differentiation of pepsinogen-producing cells in the stomach of Xenopus laevis in vitro.
1998,
Pubmed
,
Xenbase
Ishizuya-Oka,
Thyroid hormone-induced expression of sonic hedgehog correlates with adult epithelial development during remodeling of the Xenopus stomach and intestine.
2001,
Pubmed
,
Xenbase
Ishizuya-Oka,
Connective tissue is involved in adult epithelial development of the small intestine during anuran metamorphosis in vitro.
1992,
Pubmed
Ishizuya-Oka,
Apoptosis and cell proliferation in the Xenopus small intestine during metamorphosis.
1996,
Pubmed
,
Xenbase
Kikuyama,
Aspects of amphibian metamorphosis: hormonal control.
1993,
Pubmed
,
Xenbase
McAvoy,
Cell specialization in the small intestinal epithelium of adult Xenopus laevis: structural aspects.
1978,
Pubmed
,
Xenbase
Potten,
Identification of a putative intestinal stem cell and early lineage marker; musashi-1.
2003,
Pubmed
Sakamoto,
Role for cGATA-5 in transcriptional regulation of the embryonic chicken pepsinogen gene by epithelial-mesenchymal interactions in the developing chicken stomach.
2000,
Pubmed
Shi,
The earliest changes in gene expression in tadpole intestine induced by thyroid hormone.
1993,
Pubmed
,
Xenbase
Shi,
Thyroid hormone regulation of apoptotic tissue remodeling: implications from molecular analysis of amphibian metamorphosis.
2001,
Pubmed
,
Xenbase
Shi,
Biphasic intestinal development in amphibians: embryogenesis and remodeling during metamorphosis.
1996,
Pubmed
,
Xenbase
Shimizu,
Isolation of connective-tissue-specific genes involved in Xenopus intestinal remodeling: thyroid hormone up-regulates Tolloid/BMP-1 expression.
2002,
Pubmed
,
Xenbase
Wang,
Thyroid hormone-induced gene expression program for amphibian tail resorption.
1993,
Pubmed
,
Xenbase
Yasugi,
Gene transfer into chicken embryos as an effective system of analysis in developmental biology.
2000,
Pubmed
