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Mol Cell Biol
2000 Mar 01;206:2167-75. doi: 10.1128/MCB.20.6.2167-2175.2000.
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The H3-H4 N-terminal tail domains are the primary mediators of transcription factor IIIA access to 5S DNA within a nucleosome.
Vitolo JM
,
Thiriet C
,
Hayes JJ
.
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Reconstitution of a DNA fragment containing a Xenopus borealis somatic type 5S rRNA gene into a nucleosome greatly restricts the binding of transcription factor IIIA (TFIIIA) to its cognate DNA sequence within the internal promoter of the gene. Removal of all core histone tail domains by limited trypsin proteolysis or acetylation of the core histone tails significantly relieves this inhibition and allows TFIIIA to exhibit high-affinity binding to nucleosomal DNA. Since only a single tail or a subset of tails may be primarily responsible for this effect, we determined whether removal of the individual tail domains of the H2A-H2B dimer or the H3-H4 tetramer affects TFIIIA binding to its cognate DNA site within the 5S nucleosome in vitro. The results show that the tail domains of H3 and H4, but not those of H2A and/or H2B, directly modulate the ability of TFIIIA to bind nucleosomal DNA. In vitro transcription assays carried out with nucleosomal templates lacking individual tail domains show that transcription efficiency parallels the binding of TFIIIA. In addition, we show that the stoichiometry of core histones within the 5S DNA-core histone-TFIIIA triple complex is not changed upon TFIIIA association. Thus, TFIIIA binding occurs by displacement of H2A-H2B-DNA contacts but without complete loss of the dimer from the nucleoprotein complex. These data, coupled with previous reports (M. Vettese-Dadey, P. A. Grant, T. R. Hebbes, C. Crane-Robinson, C. D. Allis, and J. L. Workman, EMBO J. 15:2508-2518, 1996; L. Howe, T. A. Ranalli, C. D. Allis, and J. Ausio, J. Biol. Chem. 273:20693-20696, 1998), suggest that the H3/H4 tails are the primary arbiters of transcription factor access to intranucleosomal DNA.
Allan,
Participation of core histone "tails" in the stabilization of the chromatin solenoid.
1982, Pubmed
Allan,
Participation of core histone "tails" in the stabilization of the chromatin solenoid.
1982,
Pubmed
Andrews,
Transient activation of oocyte 5S RNA genes in Xenopus embryos by raising the level of the trans-acting factor TFIIIA.
1987,
Pubmed
,
Xenbase
Ausio,
Use of selectively trypsinized nucleosome core particles to analyze the role of the histone "tails" in the stabilization of the nucleosome.
1989,
Pubmed
Bauer,
Nucleosome structural changes due to acetylation.
1994,
Pubmed
,
Xenbase
Böhm,
Proteolytic digestion studies of chromatin core-histone structure. Identification of limit peptides from histone H2B.
1982,
Pubmed
Bouvet,
Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1.
1994,
Pubmed
,
Xenbase
Brownell,
Special HATs for special occasions: linking histone acetylation to chromatin assembly and gene activation.
1996,
Pubmed
Cary,
Effect of acetylation on the binding of N-terminal peptides of histone H4 to DNA.
1982,
Pubmed
Clark,
Superhelical stress and nucleosome-mediated repression of 5S RNA gene transcription in vitro.
1991,
Pubmed
,
Xenbase
Del Río,
High yield purification of active transcription factor IIIA expressed in E. coli.
1991,
Pubmed
,
Xenbase
Felsenfeld,
Chromatin as an essential part of the transcriptional mechanism.
1992,
Pubmed
Flaus,
Mapping nucleosome position at single base-pair resolution by using site-directed hydroxyl radicals.
1996,
Pubmed
Garcia-Ramirez,
Modulation of chromatin folding by histone acetylation.
1995,
Pubmed
Garcia-Ramirez,
Role of the histone "tails" in the folding of oligonucleosomes depleted of histone H1.
1992,
Pubmed
Godde,
The amino-terminal tails of the core histones and the translational position of the TATA box determine TBP/TFIIA association with nucleosomal DNA.
1995,
Pubmed
,
Xenbase
Gottesfeld,
Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
1982,
Pubmed
,
Xenbase
Grunstein,
Histones as regulators of genes.
1992,
Pubmed
Hamiche,
ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF.
1999,
Pubmed
Hansen,
A role for histones H2A/H2B in chromatin folding and transcriptional repression.
1994,
Pubmed
,
Xenbase
Hansen,
Structure and function of the core histone N-termini: more than meets the eye.
1998,
Pubmed
Hayes,
Contacts of the globular domain of histone H5 and core histones with DNA in a "chromatosome".
1994,
Pubmed
,
Xenbase
Hayes,
Histone contributions to the structure of DNA in the nucleosome.
1991,
Pubmed
,
Xenbase
Hayes,
The structure of DNA in a nucleosome.
1990,
Pubmed
,
Xenbase
Hayes,
Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.
1992,
Pubmed
,
Xenbase
Howe,
Nucleosome translational position, not histone acetylation, determines TFIIIA binding to nucleosomal Xenopus laevis 5S rRNA genes.
1998,
Pubmed
,
Xenbase
Howe,
Transcriptionally active Xenopus laevis somatic 5 S ribosomal RNA genes are packaged with hyperacetylated histone H4, whereas transcriptionally silent oocyte genes are not.
1998,
Pubmed
,
Xenbase
Kuo,
Roles of histone acetyltransferases and deacetylases in gene regulation.
1998,
Pubmed
Lassar,
Transcription of class III genes: formation of preinitiation complexes.
1983,
Pubmed
,
Xenbase
Lee,
Linker DNA and H1-dependent reorganization of histone-DNA interactions within the nucleosome.
1998,
Pubmed
,
Xenbase
Lee,
A positive role for histone acetylation in transcription factor access to nucleosomal DNA.
1993,
Pubmed
,
Xenbase
Lee,
The N-terminal tail of histone H2A binds to two distinct sites within the nucleosome core.
1997,
Pubmed
,
Xenbase
Lefebvre,
Binding of retinoic acid receptor heterodimers to DNA. A role for histones NH2 termini.
1998,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Norton,
Nucleosome linking number change controlled by acetylation of histones H3 and H4.
1990,
Pubmed
Norton,
Histone acetylation reduces nucleosome core particle linking number change.
1989,
Pubmed
Panetta,
Differential nucleosome positioning on Xenopus oocyte and somatic 5 S RNA genes determines both TFIIIA and H1 binding: a mechanism for selective H1 repression.
1998,
Pubmed
,
Xenbase
Pennings,
Linker histones H1 and H5 prevent the mobility of positioned nucleosomes.
1994,
Pubmed
Polach,
Mechanism of protein access to specific DNA sequences in chromatin: a dynamic equilibrium model for gene regulation.
1995,
Pubmed
Polach,
A model for the cooperative binding of eukaryotic regulatory proteins to nucleosomal target sites.
1996,
Pubmed
Pruss,
Histone-DNA contacts in a nucleosome core containing a Xenopus 5S rRNA gene.
1993,
Pubmed
,
Xenbase
Rhodes,
Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
1985,
Pubmed
,
Xenbase
Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase
Schwarz,
Formation and stability of higher order chromatin structures. Contributions of the histone octamer.
1994,
Pubmed
Sera,
Role of histone H1 as an architectural determinant of chromatin structure and as a specific repressor of transcription on Xenopus oocyte 5S rRNA genes.
1998,
Pubmed
,
Xenbase
Simpson,
Nucleosome positioning can affect the function of a cis-acting DNA element in vivo.
1990,
Pubmed
Smith,
Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.
1984,
Pubmed
,
Xenbase
Straka,
A functional role for nucleosomes in the repression of a yeast promoter.
1991,
Pubmed
Thiriet,
Antisera directed against anti-histone H4 antibodies recognize linker histones. Novel immunological probes to detect histone interactions.
1997,
Pubmed
Thiriet,
Functionally relevant histone-DNA interactions extend beyond the classically defined nucleosome core region.
1998,
Pubmed
,
Xenbase
Thiriet,
Rapid and effective western blotting of histones from acid-urea-Triton and sodium dodecyl sulfate polyacrylamide gels: two different approaches depending on the subsequent qualitative or quantitative analysis.
1995,
Pubmed
Tomaszewski,
Both the 5S rRNA gene and the AT-rich flanks of xenopus laevis oocyte-type 5S rDNA repeat are required for histone H1-dependent repression of transcription of pol III-type genes in in vitro reconstituted chromatin.
1998,
Pubmed
,
Xenbase
Tremethick,
The transcription complex of the 5 S RNA gene, but not transcription factor IIIA alone, prevents nucleosomal repression of transcription.
1990,
Pubmed
,
Xenbase
Tse,
Disruption of higher-order folding by core histone acetylation dramatically enhances transcription of nucleosomal arrays by RNA polymerase III.
1998,
Pubmed
,
Xenbase
Tse,
Enhanced transcription factor access to arrays of histone H3/H4 tetramer.DNA complexes in vitro: implications for replication and transcription.
1998,
Pubmed
Ura,
A positive role for nucleosome mobility in the transcriptional activity of chromatin templates: restriction by linker histones.
1995,
Pubmed
,
Xenbase
Vettese-Dadey,
Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro.
1996,
Pubmed
Vettese-Dadey,
Role of the histone amino termini in facilitated binding of a transcription factor, GAL4-AH, to nucleosome cores.
1994,
Pubmed
Wolffe,
Chromatin disruption and modification.
1999,
Pubmed
Wolffe,
Developmental regulation of two 5S ribosomal RNA genes.
1988,
Pubmed
,
Xenbase
