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Mol Cell Biol
2005 Nov 01;2522:9886-96. doi: 10.1128/MCB.25.22.9886-9896.2005.
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The histone fold subunits of Drosophila CHRAC facilitate nucleosome sliding through dynamic DNA interactions.
Hartlepp KF
,
Fernández-Tornero C
,
Eberharter A
,
Grüne T
,
Müller CW
,
Becker PB
.
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The chromatin accessibility complex (CHRAC) is an abundant, evolutionarily conserved nucleosome remodeling machinery able to catalyze histone octamer sliding on DNA. CHRAC differs from the related ACF complex by the presence of two subunits with molecular masses of 14 and 16 kDa, whose structure and function were not known. We determined the structure of Drosophila melanogaster CHRAC14-CHRAC16 by X-ray crystallography at 2.4-angstroms resolution and found that they dimerize via a variant histone fold in a typical handshake structure. In further analogy to histones, CHRAC14-16 contain unstructured N- and C-terminal tail domains that protrude from the handshake structure. A dimer of CHRAC14-16 can associate with the N terminus of ACF1, thereby completing CHRAC. Low-affinity interactions of CHRAC14-16 with DNA significantly improve the efficiency of nucleosome mobilization by limiting amounts of ACF. Deletion of the negatively charged C terminus of CHRAC16 enhances DNA binding 25-fold but leads to inhibition of nucleosome sliding, in striking analogy to the effect of the DNA chaperone HMGB1 on nucleosome sliding. The presence of a surface compatible with DNA interaction and the geometry of an H2A-H2B heterodimer may provide a transient acceptor site for DNA dislocated from the histone surface and therefore facilitate the nucleosome remodeling process.
Badenhorst,
Biological functions of the ISWI chromatin remodeling complex NURF.
2002, Pubmed
Badenhorst,
Biological functions of the ISWI chromatin remodeling complex NURF.
2002,
Pubmed
Baxevanis,
A variety of DNA-binding and multimeric proteins contain the histone fold motif.
1995,
Pubmed
Becker,
ATP-dependent nucleosome remodeling.
2002,
Pubmed
Birck,
Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family.
1998,
Pubmed
Bochar,
A family of chromatin remodeling factors related to Williams syndrome transcription factor.
2000,
Pubmed
Bonaldi,
The DNA chaperone HMGB1 facilitates ACF/CHRAC-dependent nucleosome sliding.
2002,
Pubmed
Bozhenok,
WSTF-ISWI chromatin remodeling complex targets heterochromatic replication foci.
2002,
Pubmed
,
Xenbase
Brünger,
Crystallography & NMR system: A new software suite for macromolecular structure determination.
1998,
Pubmed
Collaborative Computational Project, Number 4,
The CCP4 suite: programs for protein crystallography.
1994,
Pubmed
Corona,
Multiple roles for ISWI in transcription, chromosome organization and DNA replication.
2004,
Pubmed
Corona,
Two histone fold proteins, CHRAC-14 and CHRAC-16, are developmentally regulated subunits of chromatin accessibility complex (CHRAC).
2000,
Pubmed
Corona,
ISWI is an ATP-dependent nucleosome remodeling factor.
1999,
Pubmed
Eberharter,
Acf1, the largest subunit of CHRAC, regulates ISWI-induced nucleosome remodelling.
2001,
Pubmed
Eberharter,
ACF1 improves the effectiveness of nucleosome mobilization by ISWI through PHD-histone contacts.
2004,
Pubmed
Eberharter,
A nucleosome sliding assay for chromatin remodeling factors.
2004,
Pubmed
Eberharter,
ATP-dependent nucleosome remodelling: factors and functions.
2004,
Pubmed
Flaus,
Mechanisms for ATP-dependent chromatin remodelling: farewell to the tuna-can octamer?
2004,
Pubmed
Fyodorov,
Binding of Acf1 to DNA involves a WAC motif and is important for ACF-mediated chromatin assembly.
2002,
Pubmed
Gangloff,
The human TFIID components TAF(II)135 and TAF(II)20 and the yeast SAGA components ADA1 and TAF(II)68 heterodimerize to form histone-like pairs.
2000,
Pubmed
Goppelt,
A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains.
1996,
Pubmed
Grüne,
Crystal structure and functional analysis of a nucleosome recognition module of the remodeling factor ISWI.
2003,
Pubmed
Hagemeier,
The retinoblastoma protein binds E2F residues required for activation in vivo and TBP binding in vitro.
1993,
Pubmed
Hill,
Human SWI/SNF nucleosome remodeling activity is partially inhibited by linker histone H1.
2000,
Pubmed
Horn,
Phosphorylation of linker histones regulates ATP-dependent chromatin remodeling enzymes.
2002,
Pubmed
,
Xenbase
Iida,
Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae.
2004,
Pubmed
Ito,
ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor.
1997,
Pubmed
Jones,
Improved methods for building protein models in electron density maps and the location of errors in these models.
1991,
Pubmed
Kukimoto,
The histone-fold protein complex CHRAC-15/17 enhances nucleosome sliding and assembly mediated by ACF.
2004,
Pubmed
Längst,
Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer.
1999,
Pubmed
Längst,
Nucleosome remodeling: one mechanism, many phenomena?
2004,
Pubmed
Längst,
Nucleosome mobilization and positioning by ISWI-containing chromatin-remodeling factors.
2001,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Lutzmann,
Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins.
2002,
Pubmed
McConnell,
Histone fold protein Dls1p is required for Isw2-dependent chromatin remodeling in vivo.
2004,
Pubmed
Mellor,
ISWI complexes in Saccharomyces cerevisiae.
2004,
Pubmed
Narlikar,
Cooperation between complexes that regulate chromatin structure and transcription.
2002,
Pubmed
Pennings,
Linker histones H1 and H5 prevent the mobility of positioned nucleosomes.
1994,
Pubmed
Poot,
HuCHRAC, a human ISWI chromatin remodelling complex contains hACF1 and two novel histone-fold proteins.
2000,
Pubmed
Romier,
The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y.
2003,
Pubmed
Santoro,
The nucleolar remodeling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription.
2002,
Pubmed
Strohner,
Recruitment of the nucleolar remodeling complex NoRC establishes ribosomal DNA silencing in chromatin.
2004,
Pubmed
Terwilliger,
Automated MAD and MIR structure solution.
1999,
Pubmed
Terwilliger,
Maximum-likelihood density modification.
2000,
Pubmed
Travers,
Priming the nucleosome: a role for HMGB proteins?
2003,
Pubmed
Varga-Weisz,
Chromatin-remodelling factor CHRAC contains the ATPases ISWI and topoisomerase II.
1997,
Pubmed
Xiao,
Dual functions of largest NURF subunit NURF301 in nucleosome sliding and transcription factor interactions.
2001,
Pubmed
Zemzoumi,
NF-Y histone fold alpha1 helices help impart CCAAT specificity.
1999,
Pubmed
