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Coiled coils direct assembly of a cold-activated TRP channel.
Tsuruda PR
,
Julius D
,
Minor DL
.
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Transient receptor potential (TRP) channels mediate numerous sensory transduction processes and are thought to function as tetramers. TRP channel physiology is well studied; however, comparatively little is understood regarding TRP channel assembly. Here, we identify an autonomously folded assembly domain from the cold- and menthol-gated channel TRPM8. We show that the TRPM8 cytoplasmic C-terminal domain contains a coiled coil that is necessary for channel assembly and sufficient for tetramer formation. Cell biological experiments indicate that coiled-coil formation is required for proper channel maturation and trafficking and that the coiled-coil domain alone can act as a dominant-negative inhibitor of functional channel expression. Our data define an authentic TRP modular assembly domain, establish a clear role for coiled coils in ion channel assembly, demonstrate that coiled-coil assembly domains are a general feature of TRPM channels, and delineate a new tool that should be of general use in dissecting TRPM channel function.
Babila,
Assembly of mammalian voltage-gated potassium channels: evidence for an important role of the first transmembrane segment.
1994, Pubmed,
Xenbase
Babila,
Assembly of mammalian voltage-gated potassium channels: evidence for an important role of the first transmembrane segment.
1994,
Pubmed
,
Xenbase
Becktel,
Protein stability curves.
1987,
Pubmed
Bixby,
Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels.
1999,
Pubmed
Brauchi,
Clues to understanding cold sensation: thermodynamics and electrophysiological analysis of the cold receptor TRPM8.
2004,
Pubmed
Chevesich,
Requirement for the PDZ domain protein, INAD, for localization of the TRP store-operated channel to a signaling complex.
1997,
Pubmed
Clapham,
International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channels.
2003,
Pubmed
Clapham,
The TRP ion channel family.
2001,
Pubmed
Clapham,
TRP channels as cellular sensors.
2003,
Pubmed
Davare,
A beta2 adrenergic receptor signaling complex assembled with the Ca2+ channel Cav1.2.
2001,
Pubmed
Dempski,
Oligosaccharyl transferase: gatekeeper to the secretory pathway.
2002,
Pubmed
Deutsch,
Potassium channel ontogeny.
2002,
Pubmed
Dunphy,
Compartmental organization of the Golgi stack.
1985,
Pubmed
Edelhoch,
Spectroscopic determination of tryptophan and tyrosine in proteins.
1967,
Pubmed
Engelke,
Structural domains required for channel function of the mouse transient receptor potential protein homologue TRP1beta.
2002,
Pubmed
Erler,
Ca2+-selective transient receptor potential V channel architecture and function require a specific ankyrin repeat.
2004,
Pubmed
García-Sanz,
Identification of a tetramerization domain in the C terminus of the vanilloid receptor.
2004,
Pubmed
Gillo,
Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca2+ entry.
1996,
Pubmed
,
Xenbase
Harbury,
Crystal structure of an isoleucine-zipper trimer.
1994,
Pubmed
Harbury,
A switch between two-, three-, and four-stranded coiled coils in GCN4 leucine zipper mutants.
1993,
Pubmed
Hellwig,
Homo- and heteromeric assembly of TRPV channel subunits.
2005,
Pubmed
Hoenderop,
Homo- and heterotetrameric architecture of the epithelial Ca2+ channels TRPV5 and TRPV6.
2003,
Pubmed
,
Xenbase
Howard,
Hypothesis: a helix of ankyrin repeats of the NOMPC-TRP ion channel is the gating spring of mechanoreceptors.
2004,
Pubmed
Jenke,
C-terminal domains implicated in the functional surface expression of potassium channels.
2003,
Pubmed
,
Xenbase
Julius,
Molecular mechanisms of nociception.
2001,
Pubmed
Kanki,
A structural requirement for processing the cardiac K+ channel KCNQ1.
2004,
Pubmed
Kedei,
Analysis of the native quaternary structure of vanilloid receptor 1.
2001,
Pubmed
Kobertz,
K+ channels lacking the 'tetramerization' domain: implications for pore structure.
1999,
Pubmed
,
Xenbase
Kreusch,
Crystal structure of the tetramerization domain of the Shaker potassium channel.
1998,
Pubmed
Laue,
Sedimentation equilibrium as thermodynamic tool.
1995,
Pubmed
Launay,
TRPM4 regulates calcium oscillations after T cell activation.
2004,
Pubmed
Li,
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
1992,
Pubmed
Li,
TRP and the PDZ protein, INAD, form the core complex required for retention of the signalplex in Drosophila photoreceptor cells.
2000,
Pubmed
Liedtke,
Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor.
2000,
Pubmed
Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed
Lu,
T1-T1 interactions occur in ER membranes while nascent Kv peptides are still attached to ribosomes.
2001,
Pubmed
,
Xenbase
Ludwig,
Carboxy-terminal domain mediates assembly of the voltage-gated rat ether-à-go-go potassium channel.
1997,
Pubmed
Lupas,
Predicting coiled coils from protein sequences.
1991,
Pubmed
Lupas,
The structure of alpha-helical coiled coils.
2005,
Pubmed
Malashkevich,
The crystal structure of a five-stranded coiled coil in COMP: a prototype ion channel?
1996,
Pubmed
Marx,
Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel.
2002,
Pubmed
McKemy,
Identification of a cold receptor reveals a general role for TRP channels in thermosensation.
2002,
Pubmed
,
Xenbase
Minor,
The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel.
2000,
Pubmed
,
Xenbase
Montell,
The TRP channels, a remarkably functional family.
2002,
Pubmed
Montell,
The TRP superfamily of cation channels.
2005,
Pubmed
Nagaya,
Potassium channel alpha and beta subunits assemble in the endoplasmic reticulum.
1997,
Pubmed
Peier,
A TRP channel that senses cold stimuli and menthol.
2002,
Pubmed
Robinson,
Coupled tertiary folding and oligomerization of the T1 domain of Kv channels.
2005,
Pubmed
,
Xenbase
Rohács,
PI(4,5)P2 regulates the activation and desensitization of TRPM8 channels through the TRP domain.
2005,
Pubmed
Schaefer,
Homo- and heteromeric assembly of TRP channel subunits.
2005,
Pubmed
Schmitt,
A recessive C-terminal Jervell and Lange-Nielsen mutation of the KCNQ1 channel impairs subunit assembly.
2000,
Pubmed
,
Xenbase
Schulteis,
Subunit folding and assembly steps are interspersed during Shaker potassium channel biogenesis.
1998,
Pubmed
,
Xenbase
Schwake,
A carboxy-terminal domain determines the subunit specificity of KCNQ K+ channel assembly.
2003,
Pubmed
,
Xenbase
Shen,
Molecular recognition and assembly sequences involved in the subfamily-specific assembly of voltage-gated K+ channel subunit proteins.
1995,
Pubmed
Sotomayor,
In search of the hair-cell gating spring elastic properties of ankyrin and cadherin repeats.
2005,
Pubmed
Strübing,
TRPC1 and TRPC5 form a novel cation channel in mammalian brain.
2001,
Pubmed
Strübing,
Formation of novel TRPC channels by complex subunit interactions in embryonic brain.
2003,
Pubmed
Thebault,
Novel role of cold/menthol-sensitive transient receptor potential melastatine family member 8 (TRPM8) in the activation of store-operated channels in LNCaP human prostate cancer epithelial cells.
2005,
Pubmed
Thiyagarajan,
Activation-induced subcellular redistribution of G alpha(s) is dependent upon its unique N-terminus.
2002,
Pubmed
,
Xenbase
Tsunoda,
The organization of INAD-signaling complexes by a multivalent PDZ domain protein in Drosophila photoreceptor cells ensures sensitivity and speed of signaling.
1999,
Pubmed
Van Petegem,
Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain.
2004,
Pubmed
Voets,
The principle of temperature-dependent gating in cold- and heat-sensitive TRP channels.
2004,
Pubmed
Woolfson,
The design of coiled-coil structures and assemblies.
2005,
Pubmed
Xu,
TRPgamma, a drosophila TRP-related subunit, forms a regulated cation channel with TRPL.
2000,
Pubmed
Zerangue,
An artificial tetramerization domain restores efficient assembly of functional Shaker channels lacking T1.
2000,
Pubmed
,
Xenbase
Zhang,
Evidence that TRPM8 is an androgen-dependent Ca2+ channel required for the survival of prostate cancer cells.
2004,
Pubmed
Zhong,
The heteromeric cyclic nucleotide-gated channel adopts a 3A:1B stoichiometry.
2002,
Pubmed
Zhong,
Selective heteromeric assembly of cyclic nucleotide-gated channels.
2003,
Pubmed
