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Roles of the C terminus of Armadillo in Wingless signaling in Drosophila.
Cox RT
,
Pai LM
,
Kirkpatrick C
,
Stein J
,
Peifer M
.
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Drosophila melanogaster Armadillo and its vertebrate homolog beta-catenin play multiple roles during development. Both are components of cell-cell adherens junctions and both transduce Wingless (Wg)/Wnt intercellular signals. The current model for Wingless signaling proposes that Armadillo binds the DNA-binding protein dTCF, forming a bipartite transcription factor that activates Wingless-responsive genes. In this model, Armadillo's C-terminal domain is proposed to serve an essential role as a transcriptional activation domain. In Xenopus, however, overexpression of C-terminally truncated beta-catenin activates Wnt signaling, suggesting that the C-terminal domain might not be essential. We reexamined the function of Armadillo's C terminus in Wingless signaling. We found that C-terminally truncated mutant Armadillo has a deficit in Wg-signaling activity, even when corrected for reduced protein levels. However, we also found that Armadillo proteins lacking all or part of the C terminus retain some signaling ability if overexpressed, and that mutants lacking different portions of the C-terminal domain differ in their level of signaling ability. Finally, we found that the C terminus plays a role in Armadillo protein stability in response to Wingless signal and that the C-terminal domain can physically interact with the Arm repeat region. These data suggest that the C-terminal domain plays a complex role in Wingless signaling and that Armadillo recruits the transcriptional machinery via multiple contact sites, which act in an additive fashion.
Aberle,
Single amino acid substitutions in proteins of the armadillo gene family abolish their binding to alpha-catenin.
1996, Pubmed
Aberle,
Single amino acid substitutions in proteins of the armadillo gene family abolish their binding to alpha-catenin.
1996,
Pubmed
Bauer,
Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein.
1998,
Pubmed
Behrens,
Functional interaction of beta-catenin with the transcription factor LEF-1.
1996,
Pubmed
,
Xenbase
Brand,
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
1993,
Pubmed
Brannon,
A beta-catenin/XTcf-3 complex binds to the siamois promoter to regulate dorsal axis specification in Xenopus.
1997,
Pubmed
,
Xenbase
Brunner,
pangolin encodes a Lef-1 homologue that acts downstream of Armadillo to transduce the Wingless signal in Drosophila.
1997,
Pubmed
Cadigan,
Wnt signaling: a common theme in animal development.
1997,
Pubmed
,
Xenbase
Cavallo,
Drosophila Tcf and Groucho interact to repress Wingless signalling activity.
1998,
Pubmed
Cox,
Armadillo is required for adherens junction assembly, cell polarity, and morphogenesis during Drosophila embryogenesis.
1996,
Pubmed
Cox,
Membrane-tethered Drosophila Armadillo cannot transduce Wingless signal on its own.
1999,
Pubmed
,
Xenbase
Fagotto,
Binding to cadherins antagonizes the signaling activity of beta-catenin during axis formation in Xenopus.
1996,
Pubmed
,
Xenbase
Funayama,
Embryonic axis induction by the armadillo repeat domain of beta-catenin: evidence for intracellular signaling.
1995,
Pubmed
,
Xenbase
Gallet,
Trunk-specific modulation of wingless signalling in Drosophila by teashirt binding to armadillo.
,
Pubmed
Gallet,
The C-terminal domain of armadillo binds to hypophosphorylated teashirt to modulate wingless signalling in Drosophila.
1999,
Pubmed
Hsu,
Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin.
1998,
Pubmed
Huber,
Three-dimensional structure of the armadillo repeat region of beta-catenin.
1997,
Pubmed
Karnovsky,
Anterior axis duplication in Xenopus induced by the over-expression of the cadherin-binding protein plakoglobin.
1995,
Pubmed
,
Xenbase
Klingensmith,
The segment polarity phenotype of Drosophila involves differential tendencies toward transformation and cell death.
1989,
Pubmed
Larabell,
Establishment of the dorso-ventral axis in Xenopus embryos is presaged by early asymmetries in beta-catenin that are modulated by the Wnt signaling pathway.
1997,
Pubmed
,
Xenbase
Merriam,
Cytoplasmically anchored plakoglobin induces a WNT-like phenotype in Xenopus.
1997,
Pubmed
,
Xenbase
Miller,
Analysis of the signaling activities of localization mutants of beta-catenin during axis specification in Xenopus.
1997,
Pubmed
,
Xenbase
Orsulic,
An in vivo structure-function study of armadillo, the beta-catenin homologue, reveals both separate and overlapping regions of the protein required for cell adhesion and for wingless signaling.
1996,
Pubmed
Pai,
Drosophila alpha-catenin and E-cadherin bind to distinct regions of Drosophila Armadillo.
1996,
Pubmed
Peifer,
The segment polarity gene armadillo encodes a functionally modular protein that is the Drosophila homolog of human plakoglobin.
1990,
Pubmed
Peifer,
A role for the Drosophila segment polarity gene armadillo in cell adhesion and cytoskeletal integrity during oogenesis.
1993,
Pubmed
Peifer,
wingless signal and Zeste-white 3 kinase trigger opposing changes in the intracellular distribution of Armadillo.
1994,
Pubmed
Peifer,
Phosphorylation of the Drosophila adherens junction protein Armadillo: roles for wingless signal and zeste-white 3 kinase.
1994,
Pubmed
Peifer,
The segment polarity gene armadillo interacts with the wingless signaling pathway in both embryonic and adult pattern formation.
1991,
Pubmed
Riese,
LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic.
1997,
Pubmed
Rocheleau,
Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos.
1997,
Pubmed
Roose,
The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors.
1998,
Pubmed
,
Xenbase
Rubenstein,
Localizing the adhesive and signaling functions of plakoglobin.
1997,
Pubmed
,
Xenbase
Ruiz-Echevarria,
Making sense of nonsense in yeast.
1996,
Pubmed
Sanson,
Uncoupling cadherin-based adhesion from wingless signalling in Drosophila.
1996,
Pubmed
Suter,
Requirement for phosphorylation and localization of the Bicaudal-D protein in Drosophila oocyte differentiation.
1991,
Pubmed
Thorpe,
Wnt signaling polarizes an early C. elegans blastomere to distinguish endoderm from mesoderm.
1997,
Pubmed
van de Wetering,
Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF.
1997,
Pubmed
Vleminckx,
The C-terminal transactivation domain of beta-catenin is necessary and sufficient for signaling by the LEF-1/beta-catenin complex in Xenopus laevis.
1999,
Pubmed
,
Xenbase
