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Structural determinants of slow inactivation in human cardiac and skeletal muscle sodium channels.
Vilin YY
,
Makita N
,
George AL
,
Ruben PC
.
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Skeletal and heartmuscle excitability is based upon the pool of available sodium channels as determined by both fast and slow inactivation. Slow inactivation in hH1 sodium channels significantly differs from slow inactivation in hSkM1. The beta(1)-subunit modulates fast inactivation in human skeletal sodium channels (hSkM1) but has little effect on fast inactivation in human cardiac sodium channels (hH1). The role of the beta(1)-subunit in sodium channel slow inactivation is still unknown. We used the macropatch technique on Xenopus oocytes to study hSkM1 and hH1 slow inactivation with and without beta(1)-subunit coexpression. Our results indicate that the beta(1)-subunit is partly responsible for differences in steady-state slow inactivation between hSkM1 and hH1 channels. We also studied a sodium channel chimera, in which P-loops from each domain in hSkM1 sodium channels were replaced with corresponding regions from hH1. Our results show that these chimeras exhibit hH1-like properties of steady-state slow inactivation. These data suggest that P-loops are structural determinants of sodium channel slow inactivation, and that the beta(1)-subunit modulates slow inactivation in hSkM1 but not hH1. Changes in slow inactivation time constants in sodium channels coexpressed with the beta(1)-subunit indicate possible interactions among the beta(1)-subunit, P-loops, and the slow inactivation gate in sodium channels.
Armstrong,
Inactivation of the sodium channel. II. Gating current experiments.
1977, Pubmed
Armstrong,
Inactivation of the sodium channel. II. Gating current experiments.
1977,
Pubmed
Balser,
External pore residue mediates slow inactivation in mu 1 rat skeletal muscle sodium channels.
1996,
Pubmed
,
Xenbase
Cannon,
Modification of the Na+ current conducted by the rat skeletal muscle alpha subunit by coexpression with a human brain beta subunit.
1993,
Pubmed
Catterall,
Structure and function of voltage-gated ion channels.
1993,
Pubmed
Chandler,
Slow changes in membrane permeability and long-lasting action potentials in axons perfused with fluoride solutions.
1970,
Pubmed
Chen,
Modulation of Na+ channel inactivation by the beta 1 subunit: a deletion analysis.
1995,
Pubmed
,
Xenbase
Cummins,
Impaired slow inactivation in mutant sodium channels.
1996,
Pubmed
Featherstone,
Interaction between fast and slow inactivation in Skm1 sodium channels.
1996,
Pubmed
,
Xenbase
Fleidervish,
Slow inactivation of Na+ current and slow cumulative spike adaptation in mouse and guinea-pig neocortical neurones in slices.
1996,
Pubmed
Fozzard,
Structure and function of voltage-dependent sodium channels: comparison of brain II and cardiac isoforms.
1996,
Pubmed
Gellens,
Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel.
1992,
Pubmed
,
Xenbase
George,
Primary structure of the adult human skeletal muscle voltage-dependent sodium channel.
1992,
Pubmed
Hayward,
Defective slow inactivation of sodium channels contributes to familial periodic paralysis.
1999,
Pubmed
Hayward,
Slow inactivation differs among mutant Na channels associated with myotonia and periodic paralysis.
1997,
Pubmed
HODGKIN,
A quantitative description of membrane current and its application to conduction and excitation in nerve.
1952,
Pubmed
Hoshi,
Two types of inactivation in Shaker K+ channels: effects of alterations in the carboxy-terminal region.
1991,
Pubmed
,
Xenbase
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
Isacoff,
Putative receptor for the cytoplasmic inactivation gate in the Shaker K+ channel.
1991,
Pubmed
,
Xenbase
Isom,
Primary structure and functional expression of the beta 1 subunit of the rat brain sodium channel.
1992,
Pubmed
,
Xenbase
Isom,
Auxiliary subunits of voltage-gated ion channels.
1994,
Pubmed
Kiss,
Contribution of the selectivity filter to inactivation in potassium channels.
1999,
Pubmed
Levy,
A voltage-dependent role for K+ in recovery from C-type inactivation.
1996,
Pubmed
Makielski,
Coexpression of beta 1 with cardiac sodium channel alpha subunits in oocytes decreases lidocaine block.
1996,
Pubmed
,
Xenbase
Makita,
Molecular determinants of beta 1 subunit-induced gating modulation in voltage-dependent Na+ channels.
1996,
Pubmed
,
Xenbase
Makita,
Voltage-gated Na+ channel beta 1 subunit mRNA expressed in adult human skeletal muscle, heart, and brain is encoded by a single gene.
1994,
Pubmed
,
Xenbase
Marban,
Structure and function of voltage-gated sodium channels.
1998,
Pubmed
McCormick,
Molecular determinants of Na+ channel function in the extracellular domain of the beta1 subunit.
1998,
Pubmed
,
Xenbase
Nuss,
Functional association of the beta 1 subunit with human cardiac (hH1) and rat skeletal muscle (mu 1) sodium channel alpha subunits expressed in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
O'Reilly,
Comparison of slow inactivation in human heart and rat skeletal muscle Na+ channel chimaeras.
1999,
Pubmed
Patton,
The adult rat brain beta 1 subunit modifies activation and inactivation gating of multiple sodium channel alpha subunits.
1994,
Pubmed
,
Xenbase
Richmond,
Slow inactivation in human cardiac sodium channels.
1998,
Pubmed
,
Xenbase
Rogart,
Molecular cloning of a putative tetrodotoxin-resistant rat heart Na+ channel isoform.
1989,
Pubmed
Ruben,
Steady-state availability of sodium channels. Interactions between activation and slow inactivation.
1992,
Pubmed
Rudy,
Slow inactivation of the sodium conductance in squid giant axons. Pronase resistance.
1978,
Pubmed
Ruff,
Slow sodium channel inactivation in mammalian muscle: a possible role in regulating excitability.
1988,
Pubmed
Ruff,
Single-channel basis of slow inactivation of Na+ channels in rat skeletal muscle.
1996,
Pubmed
Salgado,
Voltage-dependent removal of sodium inactivation by N-bromoacetamide and pronase.
1985,
Pubmed
Sawczuk,
Spike frequency adaptation studied in hypoglossal motoneurons of the rat.
1995,
Pubmed
Starkus,
Modification of slow sodium inactivation in nerve after internal perfusion with trypsin.
1978,
Pubmed
Todt,
Ultra-slow inactivation in mu1 Na+ channels is produced by a structural rearrangement of the outer vestibule.
1999,
Pubmed
,
Xenbase
Townsend,
Effect of alkali metal cations on slow inactivation of cardiac Na+ channels.
1997,
Pubmed
,
Xenbase
Trimmer,
Primary structure and functional expression of a mammalian skeletal muscle sodium channel.
1989,
Pubmed
,
Xenbase
Valenzuela,
Gating of cardiac Na+ channels in excised membrane patches after modification by alpha-chymotrypsin.
1994,
Pubmed
Vedantham,
Slow inactivation does not affect movement of the fast inactivation gate in voltage-gated Na+ channels.
1998,
Pubmed
,
Xenbase
Wang,
A mutation in segment I-S6 alters slow inactivation of sodium channels.
1997,
Pubmed
West,
A cluster of hydrophobic amino acid residues required for fast Na(+)-channel inactivation.
1992,
Pubmed
,
Xenbase
Yang,
Expression of the sodium channel beta 1 subunit in rat skeletal muscle is selectively associated with the tetrodotoxin-sensitive alpha subunit isoform.
1993,
Pubmed
