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Control of apical membrane chloride permeability in the renal A6 cell line by nucleotides.
Banderali U
,
Brochiero E
,
Lindenthal S
,
Raschi C
,
Bogliolo S
,
Ehrenfeld J
.
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1. The effect of extracellular nucleotides applied on the apical side of polarised A6 cells grown on permeant filters was investigated by measuring the changes in (i) the 36Cl efflux through the apical membranes, (ii) the intracellular chloride concentrations (aCli, measured with N-(6-methoxyquinolyl) acetoethyl ester, MQAE), (iii) ICl, the short-circuit current in the absence of Na+ transport and (iv) the characteristics of the apical chloride channels using a patch-clamp approach. 2. ATP or UTP (0.1-500 microM) transiently stimulated ICl. The sequence of purinergic agonist potencies was UTP = ATP > ADP > the P2X-selective agonist beta,gamma-methylene ATP = the P2Y-selective agonist 2-methylthioATP. Suramin (100 microM) as the P2Y antagonist Reactive Blue 2 (10 microM) had no effect on the UTP (or ATP)-stimulated current. These findings are consistent with the presence of P2Y2-like receptors located on the apical membranes of A6 cells. Apical application of adenosine also transiently increased ICl. This effect was blocked by theophylline while the UTP-stimulated ICl was not. The existence of a second receptor, of the P1 type is proposed. 3. ATP (or UTP)-stimulated ICl was blocked by apical application of 200 microM N-phenylanthranilic acid (DPC) or 100 microM niflumic acid while 100 microM glibenclamide was ineffective. 4. Ionomycin and thapsigargin both transiently stimulated ICl; the nucleotide stimulation of ICl was not suppressed by pre-treatment with these agents. Chlorpromazin (50 microM), a Ca2+-calmodulin inhibitor strongly inhibited the stimulation of ICl induced either by apical UTP or by ionomycin application. BAPTA-AM pre-treatment of A6 cells blocked the UTP-stimulated ICl. Niflumic acid also blocked the ionomycin stimulated ICl. 5. A fourfold increase in 36Cl effluxes through the apical membranes was observed after ATP or UTP application. These increases of the apical chloride permeability could also be observed when following aCli changes. Apical application of DPC (1 mM) or 5-nitro-2(3-phenylpropylamino)benzoic acid (NPPB; 500 microM) produced an incomplete inhibition of 36Cl effluxes through the apical membranes in ATP-stimulated and in untreated monolayers. 6. In single channel patch-clamp experiments, an apical chloride channel with a unitary single channel conductance of 7.3 +/- 0.6 pS (n = 12) was usually observed. ATP application induced the activation of one or more of these channels within a few minutes. 7. These results indicate that multiple purinergic receptor subtypes are present in the apical membranes of A6 cells and that nucleotides can act as modulators of Cl- secretion in renal cells.
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994, Pubmed,
Xenbase
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Barnard,
Nucleotide receptors in the nervous system. An abundant component using diverse transduction mechanisms.
1997,
Pubmed
Begenisich,
Regulation of chloride channels in secretory epithelia.
1998,
Pubmed
Brochiero,
Na/Ca exchange in the basolateral membrane of the A6 cell monolayer: role in Cai homeostasis.
1995,
Pubmed
,
Xenbase
Brochiero,
Characterisation of Ca2+ membrane permeability of renal A6 cells upon different osmotic conditions.
1997,
Pubmed
,
Xenbase
Brochiero,
Basolateral membrane chloride permeability of A6 cells: implication in cell volume regulation.
1995,
Pubmed
,
Xenbase
Burnstock,
P2 purinoceptors: historical perspective and classification.
1996,
Pubmed
Chalfant,
Distinct regulation of Na+ reabsorption and Cl- secretion by arginine vasopressin in the amphibian cell line A6.
1993,
Pubmed
,
Xenbase
Chao,
Calcium- and CaMKII-dependent chloride secretion induced by the microsomal Ca(2+)-ATPase inhibitor 2,5-di-(tert-butyl)-1,4-hydroquinone in cystic fibrosis pancreatic epithelial cells.
1995,
Pubmed
Clarke,
Chloride secretory response to extracellular ATP in human normal and cystic fibrosis nasal epithelia.
1992,
Pubmed
Crowe,
Apical membrane sodium and chloride entry during osmotic swelling of renal (A6) epithelial cells.
1995,
Pubmed
Denicourt,
Evidence from incorporation experiments for an anionic channel of small conductance at the apical membrane of the rabbit distal tubule.
1996,
Pubmed
Dharmsathaphorn,
Multiple calcium-mediated effector mechanisms regulate chloride secretory responses in T84-cells.
1989,
Pubmed
Dharmsathaphorn,
Mechanism of chloride secretion induced by carbachol in a colonic epithelial cell line.
1986,
Pubmed
Dho,
Purinergic receptor activation of Cl- secretion in T84 cells.
1992,
Pubmed
Ehrenfeld,
Basolateral potassium membrane permeability of A6 cells and cell volume regulation.
1994,
Pubmed
,
Xenbase
Fan,
Identification of a regulated Na/K/Cl cotransport system in a distal nephron cell line.
1992,
Pubmed
,
Xenbase
Grynkiewicz,
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
1985,
Pubmed
Handler,
Toad urinary bladder epithelial cells in culture: maintenance of epithelial structure, sodium transport, and response to hormones.
1979,
Pubmed
Horn,
Estimating the number of channels in patch recordings.
1991,
Pubmed
Keeler,
Evidence that prostaglandin E2 stimulates chloride secretion in cultured A6 renal epithelial cells.
1986,
Pubmed
Ling,
Expression of the cystic fibrosis phenotype in a renal amphibian epithelial cell line.
1997,
Pubmed
,
Xenbase
Marunaka,
Modification of Ca(2+)-sensitivity of Ca(2+)-activated Cl- channel by vasopressin and cholera toxin.
1993,
Pubmed
Marunaka,
Effects of insulin and phosphatase on a Ca2(+)-dependent Cl- channel in a distal nephron cell line (A6).
1990,
Pubmed
Marunaka,
Chloride channels in the apical membrane of a distal nephron A6 cell line.
1990,
Pubmed
Marunaka,
Effects of vasopressin on single Cl- channels in the apical membrane of distal nephron cells (A6).
1993,
Pubmed
Middleton,
Nucleotide receptors regulate membrane ion transport in renal epithelial cells.
1993,
Pubmed
Mitchell,
A release mechanism for stored ATP in ocular ciliary epithelial cells.
1998,
Pubmed
Mori,
ATP-activated cation conductance in a Xenopus renal epithelial cell line.
1996,
Pubmed
,
Xenbase
Morris,
Microtubule disruption inhibits AVT-stimulated Cl- secretion but not Na+ reabsorption in A6 cells.
1998,
Pubmed
,
Xenbase
Nilius,
Ca2+ release and activation of K+ and Cl- currents by extracellular ATP in distal nephron epithelial cells.
1995,
Pubmed
North,
Nucleotide receptors.
1997,
Pubmed
Perkins,
Transport properties of toad kidney epithelia in culture.
1981,
Pubmed
,
Xenbase
Price,
Function of Xenopus cystic fibrosis transmembrane conductance regulator (CFTR) Cl channels and use of human-Xenopus chimeras to investigate the pore properties of CFTR.
1996,
Pubmed
,
Xenbase
Rabe,
Cl- channel inhibition by glibenclamide is not specific for the CFTR-type Cl- channel.
1995,
Pubmed
Ralevic,
Receptors for purines and pyrimidines.
1998,
Pubmed
Rugolo,
ATP and A1 adenosine receptor agonists mobilize intracellular calcium and activate K+ and Cl- currents in normal and cystic fibrosis airway epithelial cells.
1993,
Pubmed
Sariban-Sohraby,
Apical sodium uptake in toad kidney epithelial cell line A6.
1983,
Pubmed
Sheppard,
Inhibition of the cystic fibrosis transmembrane conductance regulator by ATP-sensitive K+ channel regulators.
1993,
Pubmed
Shintani,
Regulation of chloride channel trafficking by cyclic AMP via protein kinase A-independent pathway in A6 renal epithelial cells.
1996,
Pubmed
Silva,
Mechanism of active chloride secretion by shark rectal gland: role of Na-K-ATPase in chloride transport.
1977,
Pubmed
Taylor,
Bioluminescence detection of ATP release mechanisms in epithelia.
1998,
Pubmed
Verrey,
Antidiuretic hormone action in A6 cells: effect on apical Cl and Na conductances and synergism with aldosterone for NaCl reabsorption.
1994,
Pubmed
,
Xenbase
Verrey,
Regulation by aldosterone of Na+,K+-ATPase mRNAs, protein synthesis, and sodium transport in cultured kidney cells.
1987,
Pubmed
,
Xenbase
Wagner,
Activation of chloride channels in normal and cystic fibrosis airway epithelial cells by multifunctional calcium/calmodulin-dependent protein kinase.
1991,
Pubmed
Wilson,
Nucleotide-evoked calcium signals and anion secretion in equine cultured epithelia that express apical P2Y2 receptors and pyrimidine nucleotide receptors.
1998,
Pubmed
Yanase,
Adenosine 3',5'-cyclic monophosphate stimulates chloride secretion in A6 epithelia.
1986,
Pubmed
,
Xenbase
Zegarra-Moran,
Regulation of transepithelial ion transport by two different purinoceptors in the apical membrane of canine kidney (MDCK) cells.
1995,
Pubmed
