Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Adenosine inhibits the transfected Na+-H+ exchanger NHE3 in Xenopus laevis renal epithelial cells (A6/C1).
Di Sole F
,
Casavola V
,
Mastroberardino L
,
Verrey F
,
Moe OW
,
Burckhardt G
,
Murer H
,
Helmle-Kolb C
.
???displayArticle.abstract???
1. Adenosine influences the vectorial transport of Na+ and HCO3- across kidney epithelial cells. However, its action on effector proteins, such as the Na+-H+ exchanger NHE3, an epithelial brush border isoform of the Na+-H+ exchanger (NHE) gene family, is not yet defined. 2. The present study was conducted in Xenopus laevis distalnephron A6 epithelia which express both an apical adenosine receptor of the A1 type (coupled to protein kinase C (PKC)) and a basolateral receptor of the A2 type (coupled to protein kinase A (PKA)). The untransfected A6 cell line expresses a single NHE type (XNHE) which is restricted to the basolateral membrane and which is activated by PKA. 3. A6 cell lines were generated which express exogenous rat NHE3. Measurements of side-specific pHi recovery from acid loads in the presence of HOE694 (an inhibitor with differential potency towards individual NHE isoforms) detected an apical resistant Na+-H+ exchange only in transfected cell lines. The sensitivity of the basolateral NHE to HOE694 was unchanged, suggesting that exogenous NHE3 was restricted to the apical membrane. 4. Stimulation of the apical A1 receptor with N 6-cyclopentyladenosine (CPA) inhibited both apical NHE3 and basolateral XNHE. These effects were mimicked by the addition of the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) and partially prevented by the PKC inhibitor calphostin C which also blocked the effect of PMA. 5. Stimulation of the basolateral A2 receptor with CPA inhibited apical NHE3 and stimulated basolateral XNHE. These effects were mimicked by 8-bromo-cAMP and partially prevented by the PKA inhibitor H89 which entirely blocked the effect of 8-bromo-cAMP. 6. In conclusion, CPA inhibits rat NHE3 expressed apically in A6 epithelia via both the apical PKC-coupled A1 and the basolateral PKA-coupled A2 adenosine receptors.
Amemiya,
Acid incubation increases NHE-3 mRNA abundance in OKP cells.
1995,
Pubmed
,
Xenbase
Amemiya,
Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb.
1995,
Pubmed
Azarani,
Parathyroid hormone and parathyroid hormone-related peptide inhibit the apical Na+/H+ exchanger NHE-3 isoform in renal cells (OK) via a dual signaling cascade involving protein kinase A and C.
1995,
Pubmed
Beron,
Phorbol 12-myristate 13-acetate down-regulates Na,K-ATPase independent of its protein kinase C site: decrease in basolateral cell surface area.
1997,
Pubmed
,
Xenbase
Biemesderfer,
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney.
1997,
Pubmed
Borgese,
The cytoplasmic domain of the Na+/H+ exchangers (NHEs) dictates the nature of the hormonal response: behavior of a chimeric human NHE1/trout beta NHE antiporter.
1994,
Pubmed
Brewer,
Cytomegalovirus plasmid vectors for permanent lines of polarized epithelial cells.
1994,
Pubmed
Burns,
Regulation of Na(+)-H+ exchange by ATP depletion and calmodulin antagonism in renal epithelial cells.
1991,
Pubmed
Busch,
Cloning and sequencing of the cDNA encoding for a Na+/H+ exchanger from Xenopus laevis oocytes (X1-NHE).
1997,
Pubmed
,
Xenbase
Busch,
G protein regulation of the Na+/H+ antiporter in Xenopus laevis oocytes. Involvement of protein kinases A and C.
1995,
Pubmed
,
Xenbase
Cabado,
Distinct structural domains confer cAMP sensitivity and ATP dependence to the Na+/H+ exchanger NHE3 isoform.
1996,
Pubmed
Casavola,
Polarization of adenosine effects on intracellular pH in A6 renal epithelial cells.
1997,
Pubmed
,
Xenbase
Casavola,
Effect of adenosine on Na+ and Cl- currents in A6 monolayers. Receptor localization and messenger involvement.
1996,
Pubmed
Cohen,
Rabbit ileal villus cell brush border Na+/H+ exchange is regulated by Ca2+/calmodulin-dependent protein kinase II, a brush border membrane protein.
1990,
Pubmed
Friedlander,
Extracellular nucleotides as modulators of renal tubular transport.
1995,
Pubmed
Gekle,
Rapid activation of Na+/H+ exchange by aldosterone in renal epithelial cells requires Ca2+ and stimulation of a plasma membrane proton conductance.
1996,
Pubmed
Guerra,
Vasopressin-dependent control of basolateral Na/H-exchange in highly differentiated A6-cell monolayers.
1993,
Pubmed
,
Xenbase
Helmle-Kolb,
Regulation of the transfected Na+/H+-exchanger NHE3 in MDCK cells by vasotocin.
1997,
Pubmed
Helmle-Kolb,
Parathyroid hormone regulation of Na+/H+ exchange in opossum kidney cells: polarity and mechanisms.
1990,
Pubmed
Kandasamy,
Plasma membrane Na+/H+ exchanger isoforms (NHE-1, -2, and -3) are differentially responsive to second messenger agonists of the protein kinase A and C pathways.
1995,
Pubmed
Kurashima,
Identification of sites required for down-regulation of Na+/H+ exchanger NHE3 activity by cAMP-dependent protein kinase. phosphorylation-dependent and -independent mechanisms.
1997,
Pubmed
Lang,
Adenosine stimulates sodium transport in kidney A6 epithelia in culture.
1985,
Pubmed
,
Xenbase
Levine,
Kinetics and regulation of three cloned mammalian Na+/H+ exchangers stably expressed in a fibroblast cell line.
1993,
Pubmed
Mi,
Metabolism of exogenous cyclic AMP to adenosine in the rat kidney.
1995,
Pubmed
Moe,
Activation of protein kinase A acutely inhibits and phosphorylates Na/H exchanger NHE-3.
1995,
Pubmed
,
Xenbase
Montrose,
Regulation of intracellular pH by cultured opossum kidney cells.
1990,
Pubmed
Noël,
Hormonal regulation, pharmacology, and membrane sorting of vertebrate Na+/H+ exchanger isoforms.
1995,
Pubmed
Orlowski,
Na+/H+ exchangers of mammalian cells.
1997,
Pubmed
Osswald,
Adenosine and tubuloglomerular feedback.
1997,
Pubmed
Osswald,
[Hypernatremia and kidney function].
1993,
Pubmed
Osswald,
[Renal effects of adenosine: possible consequences for kidney transplantation].
1993,
Pubmed
Preston,
Dexamethasone accelerates differentiation of A6 epithelia and increases response to vasopressin.
1988,
Pubmed
,
Xenbase
Spindler,
Characterization of early aldosterone-induced RNAs identified in A6 kidney epithelia.
1997,
Pubmed
,
Xenbase
Verrey,
Antidiuretic hormone action in A6 cells: effect on apical Cl and Na conductances and synergism with aldosterone for NaCl reabsorption.
1994,
Pubmed
,
Xenbase
Wakabayashi,
Molecular physiology of vertebrate Na+/H+ exchangers.
1997,
Pubmed
Weinman,
CAMP-mediated inhibition of the renal brush border membrane Na+-H+ exchanger requires a dissociable phosphoprotein cofactor.
1993,
Pubmed
Yagil,
The effects of adenosine on transepithelial resistance and sodium uptake in the inner medullary collecting duct.
1994,
Pubmed
Yip,
Regulation of the epithelial brush border Na+/H+ exchanger isoform 3 stably expressed in fibroblasts by fibroblast growth factor and phorbol esters is not through changes in phosphorylation of the exchanger.
1997,
Pubmed
Yun,
cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein.
1997,
Pubmed
Yun,
Mammalian Na+/H+ exchanger gene family: structure and function studies.
1995,
Pubmed
Zhang,
Rapid redistribution and inhibition of renal sodium transporters during acute pressure natriuresis.
1996,
Pubmed
