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Mol Cell Biol
1999 Jan 01;191:121-35. doi: 10.1128/MCB.19.1.121.
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Spi-1/PU.1 is a positive regulator of the Fli-1 gene involved in inhibition of erythroid differentiation in friend erythroleukemic cell lines.
Starck J
,
Doubeikovski A
,
Sarrazin S
,
Gonnet C
,
Rao G
,
Skoultchi A
,
Godet J
,
Dusanter-Fourt I
,
Morle F
.
???displayArticle.abstract??? Spi-1/PU.1 and Fli-1 are two members of the ETS family of transcription factors whose expression is deregulated by proviral insertion in most erythroleukemic cell lines induced by the spleen focus-forming virus (SFFV) and Friend murine leukemia virus (F-MuLV) components of the Friend viral complex, respectively. In this study, we present evidence that transcription of the Fli-1 gene is positively regulated by Spi-1/PU.1 in SFFV-transformed cell lines: (i) all SFFV-transformed cell lines expressing Spi-1/PU.1 are characterized by a specific pattern of Fli-1 gene transcripts initiated in the -200 region instead of position -400 as reported for F-MuLV-transformed cell lines; (ii) these Fli-1 transcripts initiated in the -200 region are downregulated in parallel with that of Spi-1/PU.1 during hexamethylenebisacetamide (HMBA) induced differentiation; and (iii) Fli-1 transcription is upregulated in SFFV cells lines following stable transfection of a Spi-1/PU.1 expression vector. Furthermore, we found by transient transfection assays that the -270/-41 region of the Fli-1 gene displays promoter activity which is transactivated by Spi-1/PU.1. This promoter is strictly dependent on the integrity of two highly conserved ETS DNA binding sites that bind the Spi-1/PU.1 protein in vitro. Finally, we show that transfection of constitutive or inducible Fli-1 expression vectors in SFFV-transformed cells inhibits their erythroid differentiation induced by HMBA. Overall, these data indicate that Fli-1 is a target gene of the Spi-1/PU.1 transcription factor in SFFV-transformed cell lines. We further suggest that deregulated synthesis of Fli-1 may trigger a common mechanism contributing to erythroleukemia induced by either SFFV or F-MuLV.
Barbeau,
Characterization of the human and mouse Fli-1 promoter regions.
1996, Pubmed
Barbeau,
Characterization of the human and mouse Fli-1 promoter regions.
1996,
Pubmed
Bègue,
Identification of a second promoter in the human c-ets-2 proto-oncogene.
1997,
Pubmed
Ben-David,
Erythroleukemia induction by Friend murine leukemia virus: insertional activation of a new member of the ets gene family, Fli-1, closely linked to c-ets-1.
1991,
Pubmed
Ben-David,
Identification and mapping of a common proviral integration site Fli-1 in erythroleukemia cells induced by Friend murine leukemia virus.
1990,
Pubmed
Ben-David,
Friend virus-induced erythroleukemia and the multistage nature of cancer.
1991,
Pubmed
Casadevall,
Multimeric structure of the membrane erythropoietin receptor of murine erythroleukemia cells (Friend cells). Cross-linking of erythropoietin with the spleen focus-forming virus envelope protein.
1991,
Pubmed
Chen,
PU.1 (Spi-1) autoregulates its expression in myeloid cells.
1995,
Pubmed
Crepieux,
The two functionally distinct amino termini of chicken c-ets-1 products arise from alternative promoter usage.
1993,
Pubmed
,
Xenbase
Darby,
Functional interference between retinoic acid or steroid hormone receptors and the oncoprotein Fli-1.
1997,
Pubmed
Delgado,
Inhibition of Friend cells proliferation by spi-1 antisense oligodeoxynucleotides.
1994,
Pubmed
Doubeikovski,
Thrombopoietin-induced expression of the glycoprotein IIb gene involves the transcription factor PU.1/Spi-1 in UT7-Mpl cells.
1997,
Pubmed
Edamatsu,
Inducible high-level expression vector for mammalian cells, pEF-LAC carrying human elongation factor 1alpha promoter and lac operator.
1997,
Pubmed
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Fisher,
Role of PU.1 in hematopoiesis.
1998,
Pubmed
Galson,
Mouse beta-globin DNA-binding protein B1 is identical to a proto-oncogene, the transcription factor Spi-1/PU.1, and is restricted in expression to hematopoietic cells and the testis.
1993,
Pubmed
Gauthier,
Functional interference between the Spi-1/PU.1 oncoprotein and steroid hormone or vitamin receptors.
1993,
Pubmed
Grignani,
Negative autoregulation of c-myc gene expression is inactivated in transformed cells.
1990,
Pubmed
Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed
Hallier,
The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS.
1998,
Pubmed
Hallier,
The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb.
1996,
Pubmed
Hensold,
Expression of the transcription factor, Spi-1 (PU.1), in differentiating murine erythroleukemia cells Is regulated post-transcriptionally. Evidence for differential stability of transcription factor mRNAs following inducer exposure.
1996,
Pubmed
Howard,
Activation of the erythropoietin gene in the majority of F-MuLV-induced erythroleukemias results in growth factor independence and enhanced tumorigenicity.
1996,
Pubmed
Howard,
Temporal order and functional analysis of mutations within the Fli-1 and p53 genes during the erythroleukemias induced by F-MuLV.
1993,
Pubmed
Hromas,
Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1.
1993,
Pubmed
Jonsen,
Characterization of the cooperative function of inhibitory sequences in Ets-1.
1996,
Pubmed
Keller,
Hematopoietic commitment during embryonic stem cell differentiation in culture.
1993,
Pubmed
Klemsz,
The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene.
1990,
Pubmed
Lane,
p53: oncogene or anti-oncogene?
1990,
Pubmed
Li,
Activation of cell growth by binding of Friend spleen focus-forming virus gp55 glycoprotein to the erythropoietin receptor.
1990,
Pubmed
Mager,
The avian fli gene is specifically expressed during embryogenesis in a subset of neural crest cells giving rise to mesenchyme.
1998,
Pubmed
,
Xenbase
McKercher,
Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities.
1996,
Pubmed
Mélet,
Generation of a novel Fli-1 protein by gene targeting leads to a defect in thymus development and a delay in Friend virus-induced erythroleukemia.
1996,
Pubmed
Moreau-Gachelin,
Spi-1 is a putative oncogene in virally induced murine erythroleukaemias.
1988,
Pubmed
Moreau-Gachelin,
Spi-1/PU.1 transgenic mice develop multistep erythroleukemias.
1996,
Pubmed
Moreau-Gachelin,
Spi-1/PU.1: an oncogene of the Ets family.
1994,
Pubmed
Moreau-Gachelin,
The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias.
1989,
Pubmed
Paul,
A common site for immortalizing proviral integrations in Friend erythroleukemia: molecular cloning and characterization.
1989,
Pubmed
Quang,
Cooperation of Spi-1/PU.1 with an activated erythropoietin receptor inhibits apoptosis and Epo-dependent differentiation in primary erythroblasts and induces their Kit ligand-dependent proliferation.
1997,
Pubmed
Rao,
Deregulated expression of the PU.1 transcription factor blocks murine erythroleukemia cell terminal differentiation.
1997,
Pubmed
Ray-Gallet,
DNA binding specificities of Spi-1/PU.1 and Spi-B transcription factors and identification of a Spi-1/Spi-B binding site in the c-fes/c-fps promoter.
1995,
Pubmed
Schuetze,
Role of the PU.1 transcription factor in controlling differentiation of Friend erythroleukemia cells.
1992,
Pubmed
Schuetze,
The Ets-related transcription factor PU.1 immortalizes erythroblasts.
1993,
Pubmed
Scott,
Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages.
1994,
Pubmed
Scott,
PU.1 functions in a cell-autonomous manner to control the differentiation of multipotential lymphoid-myeloid progenitors.
1997,
Pubmed
Shivdasani,
A lineage-selective knockout establishes the critical role of transcription factor GATA-1 in megakaryocyte growth and platelet development.
1997,
Pubmed
Skoda,
Expression of c-MYC under the control of GATA-1 regulatory sequences causes erythroleukemia in transgenic mice.
1995,
Pubmed
Wasylyk,
The Ets family of transcription factors.
1993,
Pubmed
,
Xenbase
Yamada,
Overexpression of PU.1 induces growth and differentiation inhibition and apoptotic cell death in murine erythroleukemia cells.
1997,
Pubmed
Yi,
Inhibition of apoptosis by normal and aberrant Fli-1 and erg proteins involved in human solid tumors and leukemias.
1997,
Pubmed
Zhang,
The Fli-1 proto-oncogene, involved in erythroleukemia and Ewing's sarcoma, encodes a transcriptional activator with DNA-binding specificities distinct from other Ets family members.
1993,
Pubmed
Zhang,
An immunological renal disease in transgenic mice that overexpress Fli-1, a member of the ets family of transcription factor genes.
1995,
Pubmed
