XB-ART-14729
Mol Cell Biol
1998 Jul 01;187:3668-80. doi: 10.1128/MCB.18.7.3668.
Show Gene links
Show Anatomy links
Role of histone H1 as an architectural determinant of chromatin structure and as a specific repressor of transcription on Xenopus oocyte 5S rRNA genes.
Sera T
,
Wolffe AP
.
???displayArticle.abstract???
We explore the role of histone H1 as a DNA sequence-dependent architectural determinant of chromatin structure and of transcriptional activity in chromatin. The Xenopus laevis oocyte- and somatic-type 5S rRNA genes are differentially transcribed in embryonic chromosomes in vivo depending on the incorporation of somatic histone H1 into chromatin. We establish that this effect can be reconstructed at the level of a single nucleosome. H1 selectively represses oocyte-type 5S rRNA genes by directing the stable positioning of a nucleosome such that transcription factors cannot bind to the gene. This effect does not occur on the somatic-type genes. Histone H1 binds to the 5' end of the nucleosome core on the somatic 5S rRNA gene, leaving key regulatory elements in the promoter accessible, while histone H1 binds to the 3' end of the nucleosome core on the oocyte 5S rRNA genes, specifically blocking access to a key promoter element (the C box). TFIIIA can bind to the somatic 5S rRNA gene assembled into a nucleosome in the presence of H1. Because H1 binds with equivalent affinities to nucleosomes containing either gene, we establish that it is the sequence-selective assembly of a specific repressive chromatin structure on the oocyte 5S rRNA genes that accounts for differential transcriptional repression. Thus, general components of chromatin can determine the assembly of specific regulatory nucleoprotein complexes.
???displayArticle.pubmedLink??? 9632749
???displayArticle.pmcLink??? PMC108949
???displayArticle.link??? Mol Cell Biol
Species referenced: Xenopus laevis
Genes referenced: gtf3a
References [+] :
Allan,
The structure of histone H1 and its location in chromatin.
1980, Pubmed
Allan, The structure of histone H1 and its location in chromatin. 1980, Pubmed
Andrews, Transient activation of oocyte 5S RNA genes in Xenopus embryos by raising the level of the trans-acting factor TFIIIA. 1987, Pubmed , Xenbase
Arents, The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix. 1991, Pubmed
Bazett-Jones, Short-range DNA looping by the Xenopus HMG-box transcription factor, xUBF. 1994, Pubmed , Xenbase
Becker, The establishment of active promoters in chromatin. 1994, Pubmed
Birkenmeier, A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes. 1978, Pubmed , Xenbase
Bogenhagen, A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region. 1980, Pubmed , Xenbase
Bouvet, Specific regulation of Xenopus chromosomal 5S rRNA gene transcription in vivo by histone H1. 1994, Pubmed , Xenbase
Burley, Histone-like transcription factors in eukaryotes. 1997, Pubmed
Camerini-Otero, The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel. 1976, Pubmed
Carroll, Repeating units of Xenopus laevis oocyte-type 5S DNA are heterogeneous in length. 1976, Pubmed , Xenbase
Chipev, Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo. 1992, Pubmed , Xenbase
Churchill, 'SPKK' motifs prefer to bind to DNA at A/T-rich sites. 1989, Pubmed
Cirillo, Binding of the winged-helix transcription factor HNF3 to a linker histone site on the nucleosome. 1998, Pubmed
Clark, Co-crystal structure of the HNF-3/fork head DNA-recognition motif resembles histone H5. 1993, Pubmed
Cooper, The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure. 1994, Pubmed
Crane-Robinson, Where is the globular domain of linker histone located on the nucleosome? 1997, Pubmed
Croston, Sequence-specific antirepression of histone H1-mediated inhibition of basal RNA polymerase II transcription. 1991, Pubmed
Dong, DNA and protein determinants of nucleosome positioning on sea urchin 5S rRNA gene sequences in vitro. 1990, Pubmed
Edmondson, Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4. 1996, Pubmed
Engelke, Chromosomal footprinting of transcriptionally active and inactive oocyte-type 5S RNA genes of Xenopus laevis. 1990, Pubmed , Xenbase
Fedoroff, The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. I. The AT-rich spacer. 1978, Pubmed , Xenbase
Fox, DNA structural variations produced by actinomycin and distamycin as revealed by DNAase I footprinting. 1984, Pubmed
Giese, Assembly and function of a TCR alpha enhancer complex is dependent on LEF-1-induced DNA bending and multiple protein-protein interactions. 1995, Pubmed
Godde, Nucleosome assembly on CTG triplet repeats. 1996, Pubmed , Xenbase
Goppelt, A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains. 1996, Pubmed
Gottesfeld, DNA sequence-directed nucleosome reconstitution on 5S RNA genes of Xenopus laevis. 1987, Pubmed , Xenbase
Gottesfeld, Organization of 5S genes in chromatin of Xenopus laevis. 1980, Pubmed , Xenbase
Gottesfeld, Nonrandom alignment of nucleosomes on 5S RNA genes of X. laevis. 1980, Pubmed , Xenbase
Grosschedl, HMG domain proteins: architectural elements in the assembly of nucleoprotein structures. 1994, Pubmed
Grosschedl, Higher-order nucleoprotein complexes in transcription: analogies with site-specific recombination. 1995, Pubmed
Gurdon, Developmental inactivity of 5S RNA genes persists when chromosomes are cut between genes. 1982, Pubmed , Xenbase
Hayes, Locations of contacts between individual zinc fingers of Xenopus laevis transcription factor IIIA and the internal control region of a 5S RNA gene. 1992, Pubmed , Xenbase
Hayes, The interaction of transcription factors with nucleosomal DNA. 1992, Pubmed
Hayes, Preferential and asymmetric interaction of linker histones with 5S DNA in the nucleosome. 1993, Pubmed , Xenbase
Hayes, Contacts of the globular domain of histone H5 and core histones with DNA in a "chromatosome". 1994, Pubmed , Xenbase
Hayes, Site-directed cleavage of DNA by a linker histone--Fe(II) EDTA conjugate: localization of a globular domain binding site within a nucleosome. 1996, Pubmed , Xenbase
Hayes, A putative DNA binding surface in the globular domain of a linker histone is not essential for specific binding to the nucleosome. 1996, Pubmed , Xenbase
Hayes, Structure of the TFIIIA-5 S DNA complex. 1992, Pubmed , Xenbase
Hayes, The structure of DNA in a nucleosome. 1990, Pubmed , Xenbase
Jerzmanowski, Flanking sequences of Xenopus 5 S RNA genes determine differential inhibition of transcription by H1 histone in vitro. Mitotic phosphorylation of H1 decreases its inhibitory power. 1990, Pubmed , Xenbase
Juan, H1-mediated repression of transcription factor binding to a stably positioned nucleosome. 1997, Pubmed
Juan, Differential repression of transcription factor binding by histone H1 is regulated by the core histone amino termini. 1994, Pubmed
Kamakaka, Potentiation of RNA polymerase II transcription by Gal4-VP16 during but not after DNA replication and chromatin assembly. 1993, Pubmed
Kandolf, The H1A histone variant is an in vivo repressor of oocyte-type 5S gene transcription in Xenopus laevis embryos. 1994, Pubmed , Xenbase
Käs, Specific inhibition of DNA binding to nuclear scaffolds and histone H1 by distamycin. The role of oligo(dA).oligo(dT) tracts. 1989, Pubmed
Keller, Interaction of Xenopus TFIIIC with the TFIIIA.5 S RNA gene complex. 1992, Pubmed , Xenbase
Kopka, The molecular origin of DNA-drug specificity in netropsin and distamycin. 1985, Pubmed
Korn, Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes. 1978, Pubmed , Xenbase
Laybourn, Role of nucleosomal cores and histone H1 in regulation of transcription by RNA polymerase II. 1991, Pubmed
Love, Structural basis for DNA bending by the architectural transcription factor LEF-1. 1995, Pubmed
McConkey, TFIIIA binds with equal affinity to somatic and major oocyte 5S RNA genes. 1988, Pubmed , Xenbase
McPherson, Nucleosome positioning properties of the albumin transcriptional enhancer. 1996, Pubmed
McPherson, An active tissue-specific enhancer and bound transcription factors existing in a precisely positioned nucleosomal array. 1993, Pubmed
Meersseman, Mobile nucleosomes--a general behavior. 1992, Pubmed
Meersseman, Chromatosome positioning on assembled long chromatin. Linker histones affect nucleosome placement on 5 S rDNA. 1991, Pubmed
Nightingale, A single high affinity binding site for histone H1 in a nucleosome containing the Xenopus borealis 5 S ribosomal RNA gene. 1996, Pubmed , Xenbase
Noll, Action of micrococcal nuclease on chromatin and the location of histone H1. 1977, Pubmed
Oñate, The DNA-bending protein HMG-1 enhances progesterone receptor binding to its target DNA sequences. 1994, Pubmed
Owen-Hughes, Experimental analysis of chromatin function in transcription control. 1994, Pubmed
Patikoglou, Eukaryotic transcription factor-DNA complexes. 1997, Pubmed
Peck, Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro. 1987, Pubmed , Xenbase
Pennings, Linker histones H1 and H5 prevent the mobility of positioned nucleosomes. 1994, Pubmed
Perry, Genomic organization and nucleotide sequence of two distinct histone gene clusters from Xenopus laevis. Identification of novel conserved upstream sequence elements. 1985, Pubmed , Xenbase
Peterson, Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA. 1980, Pubmed , Xenbase
Pieler, The 5S gene internal control region is composed of three distinct sequence elements, organized as two functional domains with variable spacing. 1987, Pubmed , Xenbase
Pierrou, Cloning and characterization of seven human forkhead proteins: binding site specificity and DNA bending. 1994, Pubmed
Pruss, An asymmetric model for the nucleosome: a binding site for linker histones inside the DNA gyres. 1996, Pubmed , Xenbase
Pruss, Nucleosomal anatomy--where are the histones? 1995, Pubmed
Prymakowska-Bosak, Histone H1 overexpressed to high level in tobacco affects certain developmental programs but has limited effect on basal cellular functions. 1996, Pubmed
Ramakrishnan, Histone structure and the organization of the nucleosome. 1997, Pubmed
Ramakrishnan, Crystal structure of globular domain of histone H5 and its implications for nucleosome binding. 1993, Pubmed
Read, Solution structure of a DNA-binding domain from HMG1. 1993, Pubmed
Sakonju, A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region. 1980, Pubmed , Xenbase
Schild, A nucleosome-dependent static loop potentiates estrogen-regulated transcription from the Xenopus vitellogenin B1 promoter in vitro. 1993, Pubmed , Xenbase
Schlissel, The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1. 1984, Pubmed , Xenbase
Schultz, Histone H1 enhances the DNA binding activity of the transcription factor EmBP-1. 1996, Pubmed
Segall, Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III. 1980, Pubmed
Seguchi, Targeted disruption of 01H1 encoding a particular H1 histone variant causes changes in protein patterns in the DT40 chicken B cell line. 1995, Pubmed
Shen, Linker histone H1 regulates specific gene expression but not global transcription in vivo. 1996, Pubmed
Shim, Nucleosome positioning by the winged helix transcription factor HNF3. 1998, Pubmed
Shimamura, Histone H1 represses transcription from minichromosomes assembled in vitro. 1989, Pubmed , Xenbase
Shrader, Artificial nucleosome positioning sequences. 1989, Pubmed
Simon, A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite. 1979, Pubmed
Simpson, Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones. 1978, Pubmed
Simpson, Nucleosome positioning can affect the function of a cis-acting DNA element in vivo. 1990, Pubmed
Simpson, Nucleosome positioning: occurrence, mechanisms, and functional consequences. 1991, Pubmed
Simpson, Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure. 1985, Pubmed
Simpson, Structural features of a phased nucleosome core particle. 1983, Pubmed
Steinbach, Somatic linker histones cause loss of mesodermal competence in Xenopus. 1997, Pubmed , Xenbase
Straka, A functional role for nucleosomes in the repression of a yeast promoter. 1991, Pubmed
Stünkel, A nucleosome positioned in the distal promoter region activates transcription of the human U6 gene. 1997, Pubmed , Xenbase
Thanos, The high mobility group protein HMG I(Y) is required for NF-kappa B-dependent virus induction of the human IFN-beta gene. 1992, Pubmed
Tomaszewski, The AT-rich flanks of the oocyte-type 5S RNA gene of Xenopus laevis act as a strong local signal for histone H1-mediated chromatin reorganization in vitro. 1997, Pubmed , Xenbase
Ura, Differential association of HMG1 and linker histones B4 and H1 with dinucleosomal DNA: structural transitions and transcriptional repression. 1996, Pubmed , Xenbase
Ura, A positive role for nucleosome mobility in the transcriptional activity of chromatin templates: restriction by linker histones. 1995, Pubmed , Xenbase
Ura, Histone acetylation: influence on transcription, nucleosome mobility and positioning, and linker histone-dependent transcriptional repression. 1997, Pubmed , Xenbase
Van Dyke, Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II). 1982, Pubmed
Varga-Weisz, Energy-dependent chromatin accessibility and nucleosome mobility in a cell-free system. 1995, Pubmed
Vermaak, The globular domain of histone H1 is sufficient to direct specific gene repression in early Xenopus embryos. 1998, Pubmed , Xenbase
Wakefield, Cytoplasmic regulation of 5S RNA genes in nuclear-transplant embryos. 1983, Pubmed , Xenbase
Wang, Expanded CTG triplet blocks from the myotonic dystrophy gene create the strongest known natural nucleosome positioning elements. 1995, Pubmed , Xenbase
Weintraub, Histone-H1-dependent chromatin superstructures and the suppression of gene activity. 1984, Pubmed
Weir, Structure of the HMG box motif in the B-domain of HMG1. 1993, Pubmed
Werner, Architectural transcription factors: proteins that remodel DNA. 1997, Pubmed
Werner, Molecular basis of human 46X,Y sex reversal revealed from the three-dimensional solution structure of the human SRY-DNA complex. 1995, Pubmed
Wolffe, Dominant and specific repression of Xenopus oocyte 5S RNA genes and satellite I DNA by histone H1. 1989, Pubmed , Xenbase
Wolffe, Architectural transcription factors. 1994, Pubmed
Wolffe, Developmental regulation of two 5S ribosomal RNA genes. 1988, Pubmed , Xenbase
Wolffe, Nucleosome positioning and modification: chromatin structures that potentiate transcription. 1994, Pubmed , Xenbase
Wolffe, Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro. 1988, Pubmed , Xenbase
Wolffe, Initiation of transcription on nucleosomal templates. 1989, Pubmed
Wong, Structural and functional features of a specific nucleosome containing a recognition element for the thyroid hormone receptor. 1997, Pubmed , Xenbase
Wormington, Onset of 5 S RNA gene regulation during Xenopus embryogenesis. 1983, Pubmed , Xenbase
Wormington, A quantitative assay for Xenopus 5S RNA gene transcription in vitro. 1981, Pubmed , Xenbase
Wu, The locus of sequence-directed and protein-induced DNA bending. , Pubmed
Xie, Structural similarity between TAFs and the heterotetrameric core of the histone octamer. 1996, Pubmed
Young, Regular arrangement of nucleosomes on 5S rRNA genes in Xenopus laevis. 1983, Pubmed , Xenbase
Zappavigna, HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation. 1996, Pubmed
Zaret, Nucleoprotein architecture of the albumin transcriptional enhancer. 1995, Pubmed
Zhang, Eight different highly specific nucleosome phases on alpha-satellite DNA in the African green monkey. 1983, Pubmed
Zwieb, Absence of substantial bending in Xenopus laevis transcription factor IIIA-DNA complexes. 1990, Pubmed , Xenbase