Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biochem J
2005 Dec 15;392Pt 3:655-64. doi: 10.1042/BJ20050927.
Show Gene links
Show Anatomy links
Cloning and functional identification of slc5a12 as a sodium-coupled low-affinity transporter for monocarboxylates (SMCT2).
Srinivas SR
,
Gopal E
,
Zhuang L
,
Itagaki S
,
Martin PM
,
Fei YJ
,
Ganapathy V
,
Prasad PD
.
???displayArticle.abstract???
We report in the present paper, on the isolation and functional characterization of slc5a12, the twelfth member of the SLC5 gene family, from mouse kidney. The slc5a12 cDNA codes for a protein of 619 amino acids. Heterologous expression of slc5a12 cDNA in mammalian cells induces Na+-dependent transport of lactate and nicotinate. Several other short-chain monocarboxylates compete with nicotinate for the cDNA-induced transport process. Expression of slc5a12 in Xenopus oocytes induces electrogenic and Na+-dependent transport of lactate, nicotinate, propionate and butyrate. The substrate specificity of slc5a12 is similar to that of slc5a8, an Na+-coupled transporter for monocarboxylates. However, the substrate affinities of slc5a12 were much lower than those of slc5a8. slc5a12 mRNA is expressed in kidney, small intestine and skeletal muscle. In situ hybridization with sagittal sections of mouse kidney showed predominant expression of slc5a12 in the outer cortex. This is in contrast with slc5a8, which is expressed in the cortex as well as in the medulla. The physiological function of slc5a12 in the kidney is likely to mediate the reabsorption of lactate. In the intestinal tract, slc5a12 is expressed in the proximal parts, whereas slc5a8 is expressed in the distal parts. The expression of slc5a12 in the proximal parts of the intestinal tract, where there is minimal bacterial colonization, suggests that the physiological function of slc5a12 is not to mediate the absorption of short-chain monocarboxylates derived from bacterial fermentation but rather to mediate the absorption of diet-derived short-chain monocarboxylates. Based on the functional and structural similarities between slc5a8 and slc5a12, we suggest that the two transporters be designated as SMCT1 (sodium-coupled monocarboxylate transporter 1) and SMCT2 respectively.
Apparsundaram,
Molecular cloning of a human, hemicholinium-3-sensitive choline transporter.
2000, Pubmed,
Xenbase
Apparsundaram,
Molecular cloning of a human, hemicholinium-3-sensitive choline transporter.
2000,
Pubmed
,
Xenbase
Barac-Nieto,
Lactate-sodium cotransport in rat renal brush border membranes.
1980,
Pubmed
Barbarat,
Stoichiometry of the renal sodium-L-lactate cotransporter.
1988,
Pubmed
Berry,
The human osmoregulatory Na+/myo-inositol cotransporter gene (SLC5A3): molecular cloning and localization to chromosome 21.
1995,
Pubmed
Brooks,
Lactate shuttles in nature.
2002,
Pubmed
Choi,
PhyloDraw: a phylogenetic tree drawing system.
2000,
Pubmed
Coady,
The human tumour suppressor gene SLC5A8 expresses a Na+-monocarboxylate cotransporter.
2004,
Pubmed
,
Xenbase
CRAIG,
Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.
1946,
Pubmed
Diez-Sampedro,
A glucose sensor hiding in a family of transporters.
2003,
Pubmed
,
Xenbase
Ganapathy,
Biological functions of SLC5A8, a candidate tumour suppressor.
2005,
Pubmed
Gopal,
Sodium-coupled and electrogenic transport of B-complex vitamin nicotinic acid by slc5a8, a member of the Na/glucose co-transporter gene family.
2005,
Pubmed
,
Xenbase
Gopal,
Expression of slc5a8 in kidney and its role in Na(+)-coupled transport of lactate.
2004,
Pubmed
,
Xenbase
Halestrap,
The SLC16 gene family-from monocarboxylate transporters (MCTs) to aromatic amino acid transporters and beyond.
2004,
Pubmed
Halestrap,
The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation.
1999,
Pubmed
,
Xenbase
Hediger,
Homology of the human intestinal Na+/glucose and Escherichia coli Na+/proline cotransporters.
1989,
Pubmed
Hildmann,
Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles.
1980,
Pubmed
Jørgensen,
Renal transport of monocarboxylic acids. Heterogeneity of lactate-transport systems along the proximal tubule.
1984,
Pubmed
Jørgensen,
Characteristics of uptake of short chain fatty acids by luminal membrane vesicles from rabbit kidney.
1986,
Pubmed
Juel,
Current aspects of lactate exchange: lactate/H+ transport in human skeletal muscle.
2001,
Pubmed
Kekuda,
Cloning and functional characterization of a potential-sensitive, polyspecific organic cation transporter (OCT3) most abundantly expressed in placenta.
1998,
Pubmed
,
Xenbase
Mengual,
Kinetic asymmetry of renal Na+-L-lactate cotransport. Characteristic parameters and evidence for a ping pong mechanism of the trans-stimulating exchange by pyruvate.
1990,
Pubmed
Mengual,
Characterization of sodium and pyruvate interactions of the two carrier systems specific of mono- and di- or tricarboxylic acids by renal brush-border membrane vesicles.
1989,
Pubmed
Miyauchi,
Functional identification of SLC5A8, a tumor suppressor down-regulated in colon cancer, as a Na(+)-coupled transporter for short-chain fatty acids.
2004,
Pubmed
,
Xenbase
Pinke,
Cloning of the mouse sodium iodide symporter.
2001,
Pubmed
Poole,
Transport of lactate and other monocarboxylates across mammalian plasma membranes.
1993,
Pubmed
Prasad,
Cloning and functional expression of a cDNA encoding a mammalian sodium-dependent vitamin transporter mediating the uptake of pantothenate, biotin, and lipoate.
1998,
Pubmed
Roll,
New human sodium/glucose cotransporter gene (KST1): identification, characterization, and mutation analysis in ICCA (infantile convulsions and choreoathetosis) and BFIC (benign familial infantile convulsions) families.
2002,
Pubmed
Shen,
Localization of PEPT1 and PEPT2 proton-coupled oligopeptide transporter mRNA and protein in rat kidney.
1999,
Pubmed
Smanik,
Cloning of the human sodium lodide symporter.
1996,
Pubmed
Storelli,
Polar distribution of sodium-dependent and sodium-independent transport system for L-lactate in the plasma membrane of rat enterocytes.
1980,
Pubmed
Tamai,
Immunohistochemical and functional characterization of pH-dependent intestinal absorption of weak organic acids by the monocarboxylic acid transporter MCT1.
1999,
Pubmed
Tazawa,
SLC5A9/SGLT4, a new Na+-dependent glucose transporter, is an essential transporter for mannose, 1,5-anhydro-D-glucitol, and fructose.
2005,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Tosco,
An endogenous monocarboxylate transport in Xenopus laevis oocytes.
2000,
Pubmed
,
Xenbase
Wang,
Human placental Na+-dependent multivitamin transporter. Cloning, functional expression, gene structure, and chromosomal localization.
1999,
Pubmed
,
Xenbase
Wells,
Cloning of a human kidney cDNA with similarity to the sodium-glucose cotransporter.
1992,
Pubmed
,
Xenbase
Wolffram,
Sodium-dependent L-lactate uptake by bovine intestinal brush border membrane vesicles.
1988,
Pubmed
Wright,
Surprising versatility of Na+-glucose cotransporters: SLC5.
2004,
Pubmed
Wright,
Transport of carboxylic acids by renal membrane vesicles.
1985,
Pubmed
Wright,
The sodium/glucose cotransport family SLC5.
2004,
Pubmed
