Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biochem J
1997 Nov 01;327 ( Pt 3):735-9. doi: 10.1042/bj3270735.
Show Gene links
Show Anatomy links
Relative contributions of Na+-dependent phosphate co-transporters to phosphate transport in mouse kidney: RNase H-mediated hybrid depletion analysis.
Miyamoto K
,
Segawa H
,
Morita K
,
Nii T
,
Tatsumi S
,
Taketani Y
,
Takeda E
.
???displayArticle.abstract???
Reabsorption of Pi in the proximal tubule of the kidney is an important determinant of Pi homoeostasis. At least three types (types I-III) of high-affinity Na+-dependent Pi co-transporters have been identified in mammalian kidneys. The relative roles of these three types of Na+/Pi co-transporters in Pi transport in mouse kidney cortex have now been investigated by RNase H-mediated hybrid depletion. Whereas isolated brush-border membrane vesicles showed the presence of two kinetically distinct Na+/Pi co-transport systems (high Km-low Vmax and low Km-high Vmax), Xenopus oocytes, microinjected with polyadenylated [poly(A)+] RNA from mouse kidney cortex, showed only the high-affinity Pi uptake system. Kidney poly(A)+ RNA was incubated in vitro with antisense oligonucleotides corresponding to Npt-1 (type I), NaPi -7 (type II) or Glvr-1 (type III) Na+/Pi co-transporter mRNAs, and then with RNase H. Injection of such treated RNA preparations into Xenopus oocytes revealed that an NaPi-7 antisense oligonucleotide that resulted in complete degradation of NaPi-7 mRNA (as revealed by Northern blot analysis), also induced complete inhibition of Pi uptake. Degradation of Npt-1 or Glvr-1 mRNAs induced by corresponding antisense oligonucleotides had no effect on Pi transport, which was subsequently measured in oocytes. These results indicate that the type II Na+/Pi co-transporter NaPi-7 mediated most Na+-dependent Pi transport in mouse kidney cortex.
Barchfeld,
Alcohol effects on lipid bilayer permeability to protons and potassium: relation to the action of general anesthetics.
1988, Pubmed
Barchfeld,
Alcohol effects on lipid bilayer permeability to protons and potassium: relation to the action of general anesthetics.
1988,
Pubmed
Busch,
Expression of a renal type I sodium/phosphate transporter (NaPi-1) induces a conductance in Xenopus oocytes permeable for organic and inorganic anions.
1996,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Chong,
Cloning, genetic mapping, and expression analysis of a mouse renal sodium-dependent phosphate cotransporter.
1995,
Pubmed
Cohen,
The permeability of liposomes to nonelectrolytes. I. Activation energies for permeation.
1975,
Pubmed
Collins,
Molecular cloning, functional expression, tissue distribution, and in situ hybridization of the renal sodium phosphate (Na+/P(i)) transporter in the control and hypophosphatemic mouse.
1994,
Pubmed
,
Xenbase
Deamer,
Proton-hydroxide permeability of liposomes.
1983,
Pubmed
Johann,
Definition of a domain of GLVR1 which is necessary for infection by gibbon ape leukemia virus and which is highly polymorphic between species.
1993,
Pubmed
Kavanaugh,
Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters.
1994,
Pubmed
,
Xenbase
Levi,
Molecular regulation of renal phosphate transport.
1996,
Pubmed
Levi,
Low-Pi diet increases the abundance of an apical protein in rat proximal-tubular S3 segments.
1994,
Pubmed
Li,
Molecular cloning of two rat Na+/Pi cotransporters: evidence for differential tissue expression of transcripts.
1995,
Pubmed
Magagnin,
Expression cloning of human and rat renal cortex Na/Pi cotransport.
1993,
Pubmed
,
Xenbase
Mayer,
Vesicles of variable sizes produced by a rapid extrusion procedure.
1986,
Pubmed
Meyerhof,
Identification of G protein-coupled receptors by RNase H-mediated hybrid depletion using Xenopus laevis oocytes as expression system.
1990,
Pubmed
,
Xenbase
Miller,
Cloning of the cellular receptor for amphotropic murine retroviruses reveals homology to that for gibbon ape leukemia virus.
1994,
Pubmed
Minami,
Inhibition of glucose absorption by phlorizin affects intestinal functions in rats.
1993,
Pubmed
Miyamoto,
Characterization of the rabbit intestinal fructose transporter (GLUT5).
1994,
Pubmed
,
Xenbase
Miyamoto,
Cloning and functional expression of a Na(+)-dependent phosphate co-transporter from human kidney: cDNA cloning and functional expression.
1995,
Pubmed
,
Xenbase
Nakagawa,
Characterization of the defect in the Na(+)-phosphate transporter in vitamin D-resistant hypophosphatemic mice.
1991,
Pubmed
,
Xenbase
O'Hara,
Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus.
1990,
Pubmed
Olah,
The cellular receptor for gibbon ape leukemia virus is a novel high affinity sodium-dependent phosphate transporter.
1994,
Pubmed
Sorribas,
Cloning of a Na/Pi cotransporter from opossum kidney cells.
1994,
Pubmed
,
Xenbase
Tenenhouse,
Renal Na(+)-phosphate cotransporter gene expression in X-linked Hyp and Gy mice.
1996,
Pubmed
Verri,
Cloning of a rabbit renal Na-Pi cotransporter, which is regulated by dietary phosphate.
1995,
Pubmed
,
Xenbase
Werner,
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
1991,
Pubmed
,
Xenbase
