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Biochem J
1997 Oct 15;327 ( Pt 2):609-16. doi: 10.1042/bj3270609.
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Molecular cloning and expression of a chloride channel-associated protein pICln in human young red blood cells: association with actin.
Schwartz RS
,
Rybicki AC
,
Nagel RL
.
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We report the cloning and sequencing from human reticulocytes of cDNA coding for the Cl- channel-associated protein, pICln. Human reticulocyte pICln (HRpICln) cDNA encodes a protein (predicted molecular mass 26293Da) identical with human non-pigmented ciliary epithelial cell pICln. By using full-length HRpICln cDNA (approx. 1.2 kb) to probe human lymphocyte metaphase-chromosome spreads, the location of the human ICln gene was mapped to 11q13 by fluorescence in situ hybridization analysis. Polyclonal antibodies to recombinant HRpICln detected bands at approx. 43 kDa and approx. 37 kDa in both normal (AA) and sickle (SS) red blood cell (RBC) ghost membranes. In SS ghosts, and in ghosts from a patient with autoimmune haemolytic anaemia with 9.8% reticulocytes, the amount of HRpICln was increased compared with AA ghosts, suggesting that the expression or membrane assembly of HRpICln is cell age-dependent. Laser scanning confocal fluorescent microscopy immunolocalized HRpICln largely to the RBC membrane. The increased staining intensity of HRpICln in a reticulocyte-enriched AA RBC density-separated fraction is consistent with a dependence of HRpICln membrane content on cell age. HRpICln and beta-actin form stable complexes in vivo, demonstrated with the yeast two-hybrid system. Low-ionic-strength extraction of ghost membranes, which results in the extraction of the spectrin-actin cytoskeleton, also results in the extraction of HRpICln, consistent with the possibility for the association of these proteins in RBCs in vivo. The results presented here establish the presence of the Cl- channel-associated protein, pICln, in human RBCs, and raises the possibility that this protein has a role in RBC Cl- transport and volume regulation in young RBCs. Moreover the association of RBC pICln with actin offers a model in which to test interactions between RBC ion channels and the cytoskeleton.
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994, Pubmed,
Xenbase
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Anguita,
Molecular cloning of the human volume-sensitive chloride conductance regulatory protein, pICln, from ocular ciliary epithelium.
1995,
Pubmed
Brugnara,
Regulation of erythrocyte cation and water content in sickle cell anemia.
1986,
Pubmed
Brugnara,
Cell volume, K transport, and cell density in human erythrocytes.
1987,
Pubmed
Brugnara,
Membrane transport of Na and K and cell dehydration in sickle erythrocytes.
1993,
Pubmed
Buyse,
The ubiquitously expressed pICln protein forms homomeric complexes in vitro.
1996,
Pubmed
Canessa,
Volume-dependent and NEM-stimulated K+,Cl- transport is elevated in oxygenated SS, SC and CC human red cells.
1986,
Pubmed
Canessa,
Volume-stimulated, Cl(-)-dependent K+ efflux is highly expressed in young human red cells containing normal hemoglobin or HbS.
1987,
Pubmed
DODGE,
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.
1963,
Pubmed
Fabry,
Heterogeneity of red cells in the sickler: a characteristic with practical clinical and pathophysiological implications.
1982,
Pubmed
Fairbanks,
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
1971,
Pubmed
Garay,
Demonstration of a [K+,Cl-]-cotransport system in human red cells by its sensitivity to [(dihydroindenyl)oxy]alkanoic acids: regulation of cell swelling and distinction from the bumetanide-sensitive [Na+,K+,Cl-]-cotransport system.
1988,
Pubmed
Gschwentner,
Antisense oligonucleotides suppress cell-volume-induced activation of chloride channels.
1995,
Pubmed
Guan,
Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase.
1991,
Pubmed
Hall,
Evidence for the presence of volume-sensitive KCl transport in 'young' human red cells.
1986,
Pubmed
Hirsch,
Front-face fluorescence spectroscopy of hemoglobins.
1994,
Pubmed
Hoffmann,
Membrane mechanisms and intracellular signalling in cell volume regulation.
1995,
Pubmed
Joiner,
Cation transport and volume regulation in sickle red blood cells.
1993,
Pubmed
Krapivinsky,
Molecular characterization of a swelling-induced chloride conductance regulatory protein, pICln.
1994,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lauf,
Erythrocyte K-Cl cotransport: properties and regulation.
1992,
Pubmed
Low,
Structure and function of the cytoplasmic domain of band 3: center of erythrocyte membrane-peripheral protein interactions.
1986,
Pubmed
McCurdy,
Irreversibly sickled cells and red cell survival in sickle cell anemia: a study with both DF32P and 51CR.
1978,
Pubmed
Orlov,
Kinetics and peculiarities of thermal inactivation of volume-induced Na+/H+ exchange, Na+,K+,2Cl- cotransport and K+,Cl- cotransport in rat erythrocytes.
1993,
Pubmed
Paulmichl,
New mammalian chloride channel identified by expression cloning.
1992,
Pubmed
,
Xenbase
Rybicki,
Deficiency of protein 4.2 in erythrocytes from a patient with a Coombs negative hemolytic anemia. Evidence for a role of protein 4.2 in stabilizing ankyrin on the membrane.
1988,
Pubmed
Serjeant,
The irreversibly sickled cell; a determinant of haemolysis in sickle cell anaemia.
1969,
Pubmed
Shi,
Mapping of 29 YAC clones and identification of 3 YACs spanning the translocation t(3;8)(p14.2;q24.1) breakpoint at 8q24.1 in hereditary renal cell carcinoma.
1996,
Pubmed
Strange,
Cellular and molecular physiology of volume-sensitive anion channels.
1996,
Pubmed
Tanner,
Molecular and cellular biology of the erythrocyte anion exchanger (AE1).
1993,
Pubmed
Temple,
Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia.
1977,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed