Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1997 Jan 02;161:173-81. doi: 10.1093/emboj/16.1.173.
Show Gene links
Show Anatomy links
Staf, a promiscuous activator for enhanced transcription by RNA polymerases II and III.
Schaub M
,
Myslinski E
,
Schuster C
,
Krol A
,
Carbon P
.
???displayArticle.abstract??? Staf is a zinc finger protein that we recently identified as the transcriptional activator of the RNA polymerase III-transcribed selenocysteine tRNA gene. In this work we demonstrate that enhanced transcription of the majority of vertebrate snRNA and snRNA-type genes, transcribed by RNA polymerases II and III, also requires Staf. DNA binding assays and microinjection of mutant genes into Xenopus oocytes showed the presence of Staf-responsive elements in the genes for human U4C, U6, Y4 and 7SK, Xenopus U1b1, U2, U5 and MRP and mouse U6 RNAs. Using recombinant Staf, we established that it mediates the activating properties of Staf-responsive elements on RNA polymerase II and III snRNA promoters in vivo. Lastly a 19 bp consensus sequence for the Staf binding site, YY(A/T)CCC(A/G)N(A/C)AT(G/C)C(A/C)YY-RCR, was derived by binding site selection. It enabled us to identify 23 other snRNA and snRNA-type genes carrying potential Staf binding sites. Altogether, our results emphasize the prime importance of Staf as a novel activator for enhanced transcription of snRNA and snRNA-type genes.
Ach,
Cooperation between CCAAT and octamer motifs in the distal sequence element of the rat U3 small nucleolar RNA promoter.
1991, Pubmed,
Xenbase
Ach,
Cooperation between CCAAT and octamer motifs in the distal sequence element of the rat U3 small nucleolar RNA promoter.
1991,
Pubmed
,
Xenbase
Baer,
Structure and transcription of a human gene for H1 RNA, the RNA component of human RNase P.
1990,
Pubmed
Blackwell,
Sequence-specific DNA binding by the c-Myc protein.
1990,
Pubmed
Boyd,
Functional redundancy of promoter elements ensures efficient transcription of the human 7SK gene in vivo.
1995,
Pubmed
,
Xenbase
Chavrier,
The segment-specific gene Krox-20 encodes a transcription factor with binding sites in the promoter region of the Hox-1.4 gene.
1990,
Pubmed
Danzeiser,
Functional characterization of elements in a human U6 small nuclear RNA gene distal control region.
1993,
Pubmed
Gu,
Small RNA database.
1996,
Pubmed
Kazmaier,
Functional characterization of X. laevis U5 snRNA genes.
1987,
Pubmed
,
Xenbase
Kleinert,
Expression of a human 7S K RNA gene in vivo requires a novel pol III upstream element.
1990,
Pubmed
Krol,
Xenopus tropicalis U6 snRNA genes transcribed by Pol III contain the upstream promoter elements used by Pol II dependent U snRNA genes.
1987,
Pubmed
,
Xenbase
Lee,
Selenocysteine tRNA[Ser]Sec gene is ubiquitous within the animal kingdom.
1990,
Pubmed
,
Xenbase
Lund,
True genes for human U1 small nuclear RNA. Copy number, polymorphism, and methylation.
1984,
Pubmed
Mattaj,
U snRNA gene families in Xenopus laevis.
1985,
Pubmed
,
Xenbase
Mattaj,
Changing the RNA polymerase specificity of U snRNA gene promoters.
1988,
Pubmed
,
Xenbase
Murgo,
Sequence, organization and transcriptional analysis of a gene encoding a U1 snRNA from the axolotl, Ambystoma mexicanum.
1991,
Pubmed
,
Xenbase
Murphy,
Purified octamer binding transcription factors stimulate RNA polymerase III--mediated transcription of the 7SK RNA gene.
1989,
Pubmed
Myslinski,
Point mutations 5' to the tRNA selenocysteine TATA box alter RNA polymerase III transcription by affecting the binding of TBP.
1993,
Pubmed
,
Xenbase
Myslinski,
Optimal tRNA((Ser)Sec) gene activity requires an upstream SPH motif.
1992,
Pubmed
,
Xenbase
Myslinski,
Promoter strength and structure dictate module composition in RNA polymerase III transcriptional activator elements.
1993,
Pubmed
,
Xenbase
Ohshima,
Nucleotide sequences of mouse genomic loci including a gene or pseudogene for U6 (4.8S) nuclear RNA.
1981,
Pubmed
Roebuck,
Octamer and SPH motifs in the U1 enhancer cooperate to activate U1 RNA gene expression.
1990,
Pubmed
Savino,
Genes for Xenopus laevis U3 small nuclear RNA.
1992,
Pubmed
,
Xenbase
Schuster,
Staf, a novel zinc finger protein that activates the RNA polymerase III promoter of the selenocysteine tRNA gene.
1995,
Pubmed
,
Xenbase
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
Sturm,
The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
1988,
Pubmed
Suter-Crazzolara,
Organization and transient expression of the gene for human U11 snRNA.
1991,
Pubmed
Tebb,
The Xenopus laevis U2 gene distal sequence element (enhancer) is composed of four subdomains that can act independently and are partly functionally redundant.
1989,
Pubmed
,
Xenbase
Weller,
Transcription analysis of a human U4C gene: involvement of transcription factors novel to snRNA gene expression.
1988,
Pubmed
Zamrod,
U4B snRNA gene enhancer activity requires functional octamer and SPH motifs.
1990,
Pubmed
,
Xenbase
