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Proc Natl Acad Sci U S A
1996 Dec 10;9325:14659-64. doi: 10.1073/pnas.93.25.14659.
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Length suppression in histone messenger RNA 3'-end maturation: processing defects of insertion mutant premessenger RNAs can be compensated by insertions into the U7 small nuclear RNA.
Scharl EC
,
Steitz JA
.
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Efficient 3'-end processing of cell cycle-regulated mammalian histone premessenger RNAs (pre-mRNAs) requires an upstream stem-loop and a histone downstream element (HDE) that base pairs with the U7 small ribonucleoprotein. Insertions between these elements have two effects: the site of cleavage moves in concert with the HDE and processing efficiency declines. We used Xenopus oocytes to ask whether compensatory length insertions in the human U7 RNA could restore the fidelity and efficiency of processing of mouse histone insertion pre-mRNAs. An insertion of 5 nt into U7 RNA that extends its complementary to the HDE compensated for both defects in processing of a 5-nt insertion substrate; a noncomplementary insertion into U7 did not. Yet, the noncomplementary insertion mutant U7 was shown to be active on insertion substrates further mutated to allow base pairing. Our results suggest that the histone pre-mRNA becomes rigidified upstream of its HDE, allowing the bound U7 small ribonucleoprotein to measure from the HDE to the cleavage site. Such a mechanism may be common to other RNA measuring systems. To our knowledge, this is the first demonstration of length suppression in an RNA processing system.
Altman,
Recent studies of ribonuclease P.
1993, Pubmed
Altman,
Recent studies of ribonuclease P.
1993,
Pubmed
Backus,
Three distinct RNA sequence elements are required for efficient apolipoprotein B (apoB) RNA editing in vitro.
1992,
Pubmed
Baldi,
Participation of the intron in the reaction catalyzed by the Xenopus tRNA splicing endonuclease.
1992,
Pubmed
,
Xenbase
Biou,
The 2.9 A crystal structure of T. thermophilus seryl-tRNA synthetase complexed with tRNA(Ser).
1994,
Pubmed
Birchmeier,
The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA.
1983,
Pubmed
,
Xenbase
Birchmeier,
Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences.
1982,
Pubmed
Bond,
Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro.
1991,
Pubmed
Chen,
Protein-RNA interactions in an icosahedral virus at 3.0 A resolution.
1989,
Pubmed
Cho,
Decreasing the distance between the two conserved sequence elements of histone pre-messenger RNA interferes with 3' processing in vitro.
1995,
Pubmed
Chodchoy,
An intact histone 3'-processing site is required for transcription termination in a mouse histone H2a gene.
1991,
Pubmed
Cotten,
Ribozyme, antisense RNA, and antisense DNA inhibition of U7 small nuclear ribonucleoprotein-mediated histone pre-mRNA processing in vitro.
1989,
Pubmed
Cotten,
Specific contacts between mammalian U7 snRNA and histone precursor RNA are indispensable for the in vitro 3' RNA processing reaction.
1988,
Pubmed
Dominski,
The polyribosomal protein bound to the 3' end of histone mRNA can function in histone pre-mRNA processing.
1995,
Pubmed
Georgiev,
The conserved CAAGAAAGA spacer sequence is an essential element for the formation of 3' termini of the sea urchin H3 histone mRNA by RNA processing.
1985,
Pubmed
Gick,
Heat-labile regulatory factor is required for 3' processing of histone precursor mRNAs.
1987,
Pubmed
Grimm,
The low abundance of U7 snRNA is partly determined by its Sm binding site.
1993,
Pubmed
,
Xenbase
Hamm,
Functional analysis of mutant Xenopus U2 snRNAs.
1989,
Pubmed
,
Xenbase
Hanson,
Efficient extraction and partial purification of the polyribosome-associated stem-loop binding protein bound to the 3' end of histone mRNA.
1996,
Pubmed
Hoffmann,
Cell cycle-dependent regulation of histone precursor mRNA processing by modulation of U7 snRNA accessibility.
1990,
Pubmed
Hurt,
The mouse histone H2a.2 gene from chromosome 3.
1989,
Pubmed
Kirsebom,
RNase P--a 'Scarlet Pimpernel'.
1995,
Pubmed
Kiss-László,
Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.
1996,
Pubmed
Larson,
Histone H4 mRNA levels are down-regulated by 3' RNA processing during terminal differentiation of myoblasts.
1992,
Pubmed
Lau,
Dimeric structure of a human apolipoprotein B mRNA editing protein and cloning and chromosomal localization of its gene.
1994,
Pubmed
Lüscher,
RNA 3' processing regulates histone mRNA levels in a mammalian cell cycle mutant. A processing factor becomes limiting in G1-arrested cells.
1987,
Pubmed
Marzluff,
Histone 3' ends: essential and regulatory functions.
1992,
Pubmed
Mattoccia,
Site selection by the tRNA splicing endonuclease of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Melin,
Biochemical demonstration of complex formation of histone pre-mRNA with U7 small nuclear ribonucleoprotein and hairpin binding factors.
1992,
Pubmed
Mital,
Detection of UV-induced RNA:protein crosslinks in snRNPs by oligonucleotides complementary to the snRNA.
1993,
Pubmed
Mowry,
snRNP mediators of 3' end processing: functional fossils?
1988,
Pubmed
Mowry,
Both conserved signals on mammalian histone pre-mRNAs associate with small nuclear ribonucleoproteins during 3' end formation in vitro.
1987,
Pubmed
Mowry,
Identification of the human U7 snRNP as one of several factors involved in the 3' end maturation of histone premessenger RNA's.
1987,
Pubmed
Mowry,
Each of the conserved sequence elements flanking the cleavage site of mammalian histone pre-mRNAs has a distinct role in the 3'-end processing reaction.
1989,
Pubmed
Pace,
Evolutionary perspective on the structure and function of ribonuclease P, a ribozyme.
1995,
Pubmed
Pandey,
Different complexes are formed on the 3' end of histone mRNA with nuclear and polyribosomal proteins.
1991,
Pubmed
Pandey,
Point mutations in the stem-loop at the 3' end of mouse histone mRNA reduce expression by reducing the efficiency of 3' end formation.
1994,
Pubmed
Peculis,
Disruption of U8 nucleolar snRNA inhibits 5.8S and 28S rRNA processing in the Xenopus oocyte.
1993,
Pubmed
,
Xenbase
Peng,
Xenopus laevis: Practical uses in cell and molecular biology. Solutions and protocols.
1991,
Pubmed
,
Xenbase
Phillips,
Analysis of a gene cluster coding for the Xenopus laevis U7 snRNA.
1992,
Pubmed
,
Xenbase
Prives,
Use of oligonucleotides for antisense experiments in Xenopus laevis oocytes.
1991,
Pubmed
,
Xenbase
Reyes,
Substrate recognition and splice site determination in yeast tRNA splicing.
1988,
Pubmed
Riddle,
Frameshift suppression: a nucleotide addition in the anticodon of a glycine transfer RNA.
1973,
Pubmed
Scharl,
The site of 3' end formation of histone messenger RNA is a fixed distance from the downstream element recognized by the U7 snRNP.
1994,
Pubmed
Schaufele,
Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
,
Pubmed
,
Xenbase
Schock,
An auxiliary factor containing a 240-kDa protein complex is involved in apolipoprotein B RNA editing.
1996,
Pubmed
Smith,
Two-step affinity purification of U7 small nuclear ribonucleoprotein particles using complementary biotinylated 2'-O-methyl oligoribonucleotides.
1991,
Pubmed
Smith,
Oogenesis and oocyte isolation.
1991,
Pubmed
,
Xenbase
Spycher,
3' end processing of mouse histone pre-mRNA: evidence for additional base-pairing between U7 snRNA and pre-mRNA.
1994,
Pubmed
,
Xenbase
Stefanovic,
Assembly, nuclear import and function of U7 snRNPs studied by microinjection of synthetic U7 RNA into Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Strub,
Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte.
1986,
Pubmed
,
Xenbase
Teng,
Molecular cloning of an apolipoprotein B messenger RNA editing protein.
1993,
Pubmed
Vasserot,
Conserved terminal hairpin sequences of histone mRNA precursors are not involved in duplex formation with the U7 RNA but act as a target site for a distinct processing factor.
1989,
Pubmed
Wahle,
3'-end cleavage and polyadenylation of mRNA precursors.
1995,
Pubmed
Wahle,
The biochemistry of 3'-end cleavage and polyadenylation of messenger RNA precursors.
1992,
Pubmed
Williams,
Changes in the stem-loop at the 3' terminus of histone mRNA affects its nucleocytoplasmic transport and cytoplasmic regulation.
1994,
Pubmed
,
Xenbase
Williams,
The sequence of the stem and flanking sequences at the 3' end of histone mRNA are critical determinants for the binding of the stem-loop binding protein.
1995,
Pubmed
Wittop Koning,
RNAs and ribonucleoproteins in recognition and catalysis.
1994,
Pubmed
Yang,
In vitro reconstitution of apolipoprotein B RNA editing activity from recombinant APOBEC-1 and McArdle cell extracts.
1996,
Pubmed
