Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Dimerization of TWIK-1 K+ channel subunits via a disulfide bridge.
Lesage F
,
Reyes R
,
Fink M
,
Duprat F
,
Guillemare E
,
Lazdunski M
.
???displayArticle.abstract???
TWIK-1 is a new type of K+ channel with two P domains and is abundantly expressed in human heart and brain. Here we show that TWIK-1 subunits can self-associate to give dimers containing an interchain disulfide bridge. This assembly involves a 34 amino acid domain that is localized to the extracellular M1P1 linker loop. Cysteine 69 which is part of this interacting domain is implicated in the formation of the disulfide bond. Replacing this cysteine with a serine residue results in the loss of functional K+ channel expression. This is the first example of a covalent association of functional subunits in voltage-sensitive channels via a disulfide bridge.
Aldrich,
Potassium channels. New channel subunits are a turn-off.
1994, Pubmed,
Xenbase
Aldrich,
Potassium channels. New channel subunits are a turn-off.
1994,
Pubmed
,
Xenbase
Attali,
A corticosteroid-induced gene expressing an "IsK-like" K+ channel activity in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Babila,
Assembly of mammalian voltage-gated potassium channels: evidence for an important role of the first transmembrane segment.
1994,
Pubmed
,
Xenbase
Betz,
Homology and analogy in transmembrane channel design: lessons from synaptic membrane proteins.
1990,
Pubmed
Bezanilla,
Gating of Shaker K+ channels: II. The components of gating currents and a model of channel activation.
1994,
Pubmed
,
Xenbase
Chandy,
Nomenclature for mammalian potassium channel genes.
1993,
Pubmed
Cohen,
Alpha-helical coiled coils and bundles: how to design an alpha-helical protein.
1990,
Pubmed
Covarrubias,
Shaker, Shal, Shab, and Shaw express independent K+ current systems.
1991,
Pubmed
,
Xenbase
Deal,
Molecular physiology of cardiac potassium channels.
1996,
Pubmed
Doupnik,
The inward rectifier potassium channel family.
1995,
Pubmed
Duprat,
Heterologous multimeric assembly is essential for K+ channel activity of neuronal and cardiac G-protein-activated inward rectifiers.
1995,
Pubmed
,
Xenbase
Fink,
Dominant negative chimeras provide evidence for homo and heteromultimeric assembly of inward rectifier K+ channel proteins via their N-terminal end.
1996,
Pubmed
,
Xenbase
Garnier,
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
1978,
Pubmed
Guillemare,
Effects of the level of mRNA expression on biophysical properties, sensitivity to neurotoxins, and regulation of the brain delayed-rectifier K+ channels Kv1.2.
1992,
Pubmed
,
Xenbase
Heginbotham,
Mutations in the K+ channel signature sequence.
1994,
Pubmed
,
Xenbase
Isacoff,
Evidence for the formation of heteromultimeric potassium channels in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Jan,
Potassium channels and their evolving gates.
1994,
Pubmed
Ketchum,
A new family of outwardly rectifying potassium channel proteins with two pore domains in tandem.
1995,
Pubmed
,
Xenbase
Kofuji,
Evidence that neuronal G-protein-gated inwardly rectifying K+ channels are activated by G beta gamma subunits and function as heteromultimers.
1995,
Pubmed
,
Xenbase
Krapivinsky,
The G-protein-gated atrial K+ channel IKACh is a heteromultimer of two inwardly rectifying K(+)-channel proteins.
1995,
Pubmed
,
Xenbase
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Lee,
Structural determinant for assembly of mammalian K+ channels.
1994,
Pubmed
,
Xenbase
Lesage,
Are Xenopus oocytes unique in displaying functional IsK channel heterologous expression?
1993,
Pubmed
,
Xenbase
Lesage,
TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure.
1996,
Pubmed
,
Xenbase
Lesage,
A pH-sensitive yeast outward rectifier K+ channel with two pore domains and novel gating properties.
1996,
Pubmed
,
Xenbase
Lewis,
Potassium and calcium channels in lymphocytes.
1995,
Pubmed
Li,
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
1992,
Pubmed
Logothetis,
Incremental reductions of positive charge within the S4 region of a voltage-gated K+ channel result in corresponding decreases in gating charge.
1992,
Pubmed
,
Xenbase
Lu,
Electrostatic tuning of Mg2+ affinity in an inward-rectifier K+ channel.
1994,
Pubmed
,
Xenbase
MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase
MacKinnon,
Pore loops: an emerging theme in ion channel structure.
1995,
Pubmed
Nichols,
Inward rectification and implications for cardiac excitability.
1996,
Pubmed
Pongs,
Molecular biology of voltage-dependent potassium channels.
1992,
Pubmed
Pongs,
Structure-function studies on the pore of potassium channels.
1993,
Pubmed
Rehm,
Purification and subunit structure of a putative K+-channel protein identified by its binding properties for dendrotoxin I.
1988,
Pubmed
Reid,
The S. cerevisiae outwardly-rectifying potassium channel (DUK1) identifies a new family of channels with duplicated pore domains.
1996,
Pubmed
,
Xenbase
Rhodes,
Association and colocalization of K+ channel alpha- and beta-subunit polypeptides in rat brain.
1995,
Pubmed
Rudy,
Diversity and ubiquity of K channels.
1988,
Pubmed
Ruppersberg,
Heteromultimeric channels formed by rat brain potassium-channel proteins.
1990,
Pubmed
,
Xenbase
Salkoff,
An essential 'set' of K+ channels conserved in flies, mice and humans.
1992,
Pubmed
,
Xenbase
Shen,
Molecular recognition and assembly sequences involved in the subfamily-specific assembly of voltage-gated K+ channel subunit proteins.
1995,
Pubmed
Sheng,
Presynaptic A-current based on heteromultimeric K+ channels detected in vivo.
1993,
Pubmed
Wang,
Heteromultimeric K+ channels in terminal and juxtaparanodal regions of neurons.
1993,
Pubmed
,
Xenbase
Xu,
Assembly of voltage-gated potassium channels. Conserved hydrophilic motifs determine subfamily-specific interactions between the alpha-subunits.
1995,
Pubmed
Yang,
Determination of the subunit stoichiometry of an inwardly rectifying potassium channel.
1995,
Pubmed
,
Xenbase