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XB-ART-17315
Bioorg Khim 1996 Dec 01;2212:894-9.
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[A novel method of subtraction cDNA hybridization allowing cloning of genes showing differential expression in cell micropopulations].

Vasil'ev OL , Luk'ianov SA , Beliavskiĭ AV , Kazanskaia OV , Zaraĭskiĭ AG .


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A novel, efficient and simple technique that combines subtractive hybridization with kinetic enrichment is proposed for obtaining enriched cDNA. The method is based on the use of a set of special primers that allow for the selective amplification by PCR only of differentially distributed sequences. Using the proposed technique, cDNA of a new gene XEp-1, specifically expressed in the presumptive epidermis of Xenopus laevis, was cloned, starting with the stage of midgastrula.

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Species referenced: Xenopus laevis
Genes referenced: otogl2