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Subunits of the heterotrimeric transcription factor NF-Y are imported into the nucleus by distinct pathways involving importin beta and importin 13.
Kahle J
,
Baake M
,
Doenecke D
,
Albig W
.
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The transcriptional activator NF-Y is a heterotrimeric complex composed of NF-YA, NF-YB, and NF-YC, which specifically binds the CCAAT consensus present in about 30% of eukaryotic promoters. All three subunits contain evolutionarily conserved core regions, which comprise a histone fold motif (HFM) in the case of NF-YB and NF-YC. Our results of in vitro binding studies and nuclear import assays reveal two different transport mechanisms for NF-Y subunits. While NF-YA is imported by an importin beta-mediated pathway, the NF-YB/NF-YC heterodimer is translocated into the nucleus in an importin 13-dependent manner. We define a nonclassical nuclear localization signal (ncNLS) in NF-YA, and mutational analysis indicates that positively charged amino acid residues in the ncNLS are required for nuclear targeting of NF-YA. Importin beta binding is restricted to the monomeric, uncomplexed NF-YA subunit. In contrast, the nuclear import of NF-YB and NF-YC requires dimer formation. Only the NF-YB/NF-YC dimer, but not the monomeric components, are recognized by importin 13 and are imported into the nucleus. Importin 13 competes with NF-YA for binding to the NF-YB/NF-YC dimer. Our data suggest that a distinct binding platform derived from the HFM of both subunits, NF-YB/NF-YC, mediates those interactions.
Adam,
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.
1990, Pubmed,
Xenbase
Adam,
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.
1990,
Pubmed
,
Xenbase
Arents,
The histone fold: a ubiquitous architectural motif utilized in DNA compaction and protein dimerization.
1995,
Pubmed
Baake,
Characterisation of nuclear localisation signals of the four human core histones.
2001,
Pubmed
Baake,
Core histones and linker histones are imported into the nucleus by different pathways.
2001,
Pubmed
Baxevanis,
A variety of DNA-binding and multimeric proteins contain the histone fold motif.
1995,
Pubmed
Bellorini,
CCAAT binding NF-Y-TBP interactions: NF-YB and NF-YC require short domains adjacent to their histone fold motifs for association with TBP basic residues.
1997,
Pubmed
Bhattacharya,
The B subunit of the CCAAT box binding transcription factor complex (CBF/NF-Y) is essential for early mouse development and cell proliferation.
2003,
Pubmed
Bi,
DNA binding specificity of the CCAAT-binding factor CBF/NF-Y.
1997,
Pubmed
Bischoff,
RanBP1 is crucial for the release of RanGTP from importin beta-related nuclear transport factors.
1997,
Pubmed
Bolognese,
Cloning and characterization of the histone-fold proteins YBL1 and YCL1.
2000,
Pubmed
Bucher,
Weight matrix descriptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences.
1990,
Pubmed
Bäuerle,
The requirement of H1 histones for a heterodimeric nuclear import receptor.
2002,
Pubmed
Caretti,
NF-Y associates with H3-H4 tetramers and octamers by multiple mechanisms.
1999,
Pubmed
,
Xenbase
Caretti,
Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters.
2003,
Pubmed
Christophe,
Nuclear targeting of proteins: how many different signals?
2000,
Pubmed
Cokol,
Finding nuclear localization signals.
2000,
Pubmed
Conti,
Nucleocytoplasmic transport enters the atomic age.
2001,
Pubmed
Dingwall,
Nuclear targeting sequences--a consensus?
1991,
Pubmed
Dorn,
A multiplicity of CCAAT box-binding proteins.
1987,
Pubmed
Englmeier,
Receptor-mediated substrate translocation through the nuclear pore complex without nucleotide triphosphate hydrolysis.
1999,
Pubmed
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Forwood,
Nuclear import of Creb and AP-1 transcription factors requires importin-beta 1 and Ran but is independent of importin-alpha.
2001,
Pubmed
Frontini,
Cell cycle regulation of NF-YC nuclear localization.
2004,
Pubmed
Gurdon,
Methods for nuclear transplantation in amphibia.
1977,
Pubmed
Görlich,
Transport between the cell nucleus and the cytoplasm.
1999,
Pubmed
Görlich,
Characterization of Ran-driven cargo transport and the RanGTPase system by kinetic measurements and computer simulation.
2003,
Pubmed
Görlich,
Nucleocytoplasmic transport.
1996,
Pubmed
Görlich,
Identification of different roles for RanGDP and RanGTP in nuclear protein import.
1996,
Pubmed
,
Xenbase
Görlich,
Isolation of a protein that is essential for the first step of nuclear protein import.
1994,
Pubmed
,
Xenbase
Ho,
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
1989,
Pubmed
Imamoto,
Diversity in nucleocytoplasmic transport pathways.
2000,
Pubmed
Izaurralde,
The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus.
1997,
Pubmed
Jäkel,
Importin beta, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells.
1998,
Pubmed
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Kim,
Determination of functional domains in the C subunit of the CCAAT-binding factor (CBF) necessary for formation of a CBF-DNA complex: CBF-B interacts simultaneously with both the CBF-A and CBF-C subunits to form a heterotrimeric CBF molecule.
1996,
Pubmed
Kim,
Physical characterization of the purified CCAAT transcription factor, alpha-CP1.
1990,
Pubmed
Kose,
Ran-unassisted nuclear migration of a 97-kD component of nuclear pore-targeting complex.
1997,
Pubmed
Kutay,
Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex.
1997,
Pubmed
,
Xenbase
Kutay,
Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor.
1997,
Pubmed
,
Xenbase
Lei,
Protein and RNA export from the nucleus.
2002,
Pubmed
Li,
Xenopus NF-Y pre-sets chromatin to potentiate p300 and acetylation-responsive transcription from the Xenopus hsp70 promoter in vivo.
1998,
Pubmed
,
Xenbase
Macara,
Transport into and out of the nucleus.
2001,
Pubmed
Maity,
Role of the CCAAT-binding protein CBF/NF-Y in transcription.
1998,
Pubmed
Mantovani,
The molecular biology of the CCAAT-binding factor NF-Y.
1999,
Pubmed
Mantovani,
Dominant negative analogs of NF-YA.
1994,
Pubmed
Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed
McNabb,
Cloning of yeast HAP5: a novel subunit of a heterotrimeric complex required for CCAAT binding.
1995,
Pubmed
Melchior,
Mechanisms of nuclear protein import.
1995,
Pubmed
Mingot,
Importin 13: a novel mediator of nuclear import and export.
2001,
Pubmed
Mosammaparast,
Nuclear import of histone H2A and H2B is mediated by a network of karyopherins.
2001,
Pubmed
Mosammaparast,
Pathways mediating the nuclear import of histones H3 and H4 in yeast.
2002,
Pubmed
Mühlhäusser,
Multiple pathways contribute to nuclear import of core histones.
2001,
Pubmed
Nakshatri,
Subunit association and DNA binding activity of the heterotrimeric transcription factor NF-Y is regulated by cellular redox.
1996,
Pubmed
Nigg,
Nucleocytoplasmic transport: signals, mechanisms and regulation.
1997,
Pubmed
Ploski,
Paired-type homeodomain transcription factors are imported into the nucleus by karyopherin 13.
2004,
Pubmed
Pollard,
A novel receptor-mediated nuclear protein import pathway.
1996,
Pubmed
Rexach,
Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins.
1995,
Pubmed
Ribbeck,
Kinetic analysis of translocation through nuclear pore complexes.
2001,
Pubmed
Ribbeck,
NTF2 mediates nuclear import of Ran.
1998,
Pubmed
Ribbeck,
The translocation of transportin-cargo complexes through nuclear pores is independent of both Ran and energy.
1999,
Pubmed
Robbins,
Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence.
1991,
Pubmed
,
Xenbase
Romier,
The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y.
2003,
Pubmed
Rout,
The nuclear pore complex as a transport machine.
2001,
Pubmed
Rupp,
Xenopus embryos regulate the nuclear localization of XMyoD.
1994,
Pubmed
,
Xenbase
Schwamborn,
The histone H1(0) contains multiple sequence elements for nuclear targeting.
1998,
Pubmed
Schwoebel,
Ran-dependent signal-mediated nuclear import does not require GTP hydrolysis by Ran.
1998,
Pubmed
Sinha,
Three classes of mutations in the A subunit of the CCAAT-binding factor CBF delineate functional domains involved in the three-step assembly of the CBF-DNA complex.
1996,
Pubmed
Sinha,
Recombinant rat CBF-C, the third subunit of CBF/NFY, allows formation of a protein-DNA complex with CBF-A and CBF-B and with yeast HAP2 and HAP3.
1995,
Pubmed
Steidl,
A single subunit of a heterotrimeric CCAAT-binding complex carries a nuclear localization signal: piggy back transport of the pre-assembled complex to the nucleus.
2004,
Pubmed
Steidl,
AnCF, the CCAAT binding complex of Aspergillus nidulans, contains products of the hapB, hapC, and hapE genes and is required for activation by the pathway-specific regulatory gene amdR.
1999,
Pubmed
Ström,
Importin-beta-like nuclear transport receptors.
2001,
Pubmed
Tommasi,
In vivo structure of the human cdc2 promoter: release of a p130-E2F-4 complex from sequences immediately upstream of the transcription initiation site coincides with induction of cdc2 expression.
1995,
Pubmed
Truant,
The arginine-rich domains present in human immunodeficiency virus type 1 Tat and Rev function as direct importin beta-dependent nuclear localization signals.
1999,
Pubmed
Turner,
Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate.
1994,
Pubmed
,
Xenbase
Weis,
Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle.
2003,
Pubmed
Weis,
Characterization of the nuclear protein import mechanism using Ran mutants with altered nucleotide binding specificities.
1996,
Pubmed
Weis,
Nucleocytoplasmic transport: cargo trafficking across the border.
2002,
Pubmed
Wischnewski,
Signals and receptors for the nuclear transport of TFIIIA in Xenopus oocytes.
2004,
Pubmed
,
Xenbase
Wozniak,
Karyopherins and kissing cousins.
1998,
Pubmed
Xiao,
Importin beta mediates nuclear translocation of Smad 3.
2000,
Pubmed
Xing,
Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain.
1993,
Pubmed
Zhu,
E2Fs link the control of G1/S and G2/M transcription.
2004,
Pubmed
de Silvio,
Dissection of the NF-Y transcriptional activation potential.
1999,
Pubmed
