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Proc Natl Acad Sci U S A
1996 Nov 12;9323:13256-61. doi: 10.1073/pnas.93.23.13256.
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ORK1, a potassium-selective leak channel with two pore domains cloned from Drosophila melanogaster by expression in Saccharomyces cerevisiae.
Goldstein SA
,
Price LA
,
Rosenthal DN
,
Pausch MH
.
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A K+ channel gene has been cloned from Drosophila melanogaster by complementation in Saccharomyces cerevisiae cells defective for K+ uptake. Naturally expressed in the neuromuscular tissues of adult flies, this gene confers K+ transport capacity on yeast cells when heterologously expressed. In Xenopus laevis oocytes, expression yields an ungated K(+)-selective current whose attributes resemble the "leak" conductance thought to mediate the resting potential of vertebrate myelinated neurons but whose molecular nature has long remained elusive. The predicted protein has two pore (P) domains and four membrane-spanning helices and is a member of a newly recognized K+ channel family. Expression of the channel in flies and yeast cells makes feasible studies of structure and in vivo function using genetic approaches that are not possible in higher animals.
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992, Pubmed
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992,
Pubmed
Atkinson,
A component of calcium-activated potassium channels encoded by the Drosophila slo locus.
1991,
Pubmed
Baker,
Function and distribution of three types of rectifying channel in rat spinal root myelinated axons.
1987,
Pubmed
Baukrowitz,
Modulation of K+ current by frequency and external [K+]: a tale of two inactivation mechanisms.
1995,
Pubmed
Bennetzen,
The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
1982,
Pubmed
Brüggemann,
Ether-à-go-go encodes a voltage-gated channel permeable to K+ and Ca2+ and modulated by cAMP.
1993,
Pubmed
,
Xenbase
Butler,
A family of putative potassium channel genes in Drosophila.
1989,
Pubmed
Campbell,
Kinetic and pharmacological properties of the sodium channel of frog skeletal muscle.
1976,
Pubmed
Carmeliet,
K+ channels in cardiac cells: mechanisms of activation, inactivation, rectification and K+e sensitivity.
1989,
Pubmed
Cavener,
Eukaryotic start and stop translation sites.
1991,
Pubmed
Chiu,
A quantitative description of membrane currents in rabbit myelinated nerve.
1979,
Pubmed
Coronado,
Conduction and block by organic cations in a K+-selective channel from sarcoplasmic reticulum incorporated into planar phospholipid bilayers.
1982,
Pubmed
Elledge,
Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.
1991,
Pubmed
FRANKENHAEUSER,
A QUANTITATIVE DESCRIPTION OF POTASSIUM CURRENTS IN MYELINATED NERVE FIBRES OF XENOPUS LAEVIS.
1963,
Pubmed
,
Xenbase
FRANKENHAEUSER,
The after-effects of impulses in the giant nerve fibres of Loligo.
1956,
Pubmed
Fakler,
Strong voltage-dependent inward rectification of inward rectifier K+ channels is caused by intracellular spermine.
1995,
Pubmed
,
Xenbase
Fozzard,
The electrophysiology of acute myocardial ischemia.
1985,
Pubmed
Glowatzki,
Subunit-dependent assembly of inward-rectifier K+ channels.
1995,
Pubmed
,
Xenbase
Goldman,
POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANES.
1943,
Pubmed
HODGKIN,
A quantitative description of membrane current and its application to conduction and excitation in nerve.
1952,
Pubmed
HODGKIN,
The effect of sodium ions on the electrical activity of giant axon of the squid.
1949,
Pubmed
Heginbotham,
Mutations in the K+ channel signature sequence.
1994,
Pubmed
,
Xenbase
Heinemann,
Extracellular calcium and potassium concentration changes in chronic epileptic brain tissue.
1986,
Pubmed
Jan,
Potassium channels and their evolving gates.
1994,
Pubmed
Kamb,
Molecular characterization of Shaker, a Drosophila gene that encodes a potassium channel.
1987,
Pubmed
Ketchum,
A new family of outwardly rectifying potassium channel proteins with two pore domains in tandem.
1995,
Pubmed
,
Xenbase
Knoll,
The early evolution of eukaryotes: a geological perspective.
1992,
Pubmed
Ko,
TRK1 and TRK2 encode structurally related K+ transporters in Saccharomyces cerevisiae.
1991,
Pubmed
Kozak,
An analysis of vertebrate mRNA sequences: intimations of translational control.
1991,
Pubmed
Krapivinsky,
The G-protein-gated atrial K+ channel IKACh is a heteromultimer of two inwardly rectifying K(+)-channel proteins.
1995,
Pubmed
,
Xenbase
Kubo,
Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel.
1993,
Pubmed
,
Xenbase
Lesage,
TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure.
1996,
Pubmed
,
Xenbase
Lopatin,
Potassium channel block by cytoplasmic polyamines as the mechanism of intrinsic rectification.
1994,
Pubmed
,
Xenbase
Loughney,
Molecular analysis of the para locus, a sodium channel gene in Drosophila.
1989,
Pubmed
Lu,
Electrostatic tuning of Mg2+ affinity in an inward-rectifier K+ channel.
1994,
Pubmed
,
Xenbase
López-Barneo,
Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels.
1993,
Pubmed
,
Xenbase
MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase
MacKinnon,
Pore loops: an emerging theme in ion channel structure.
1995,
Pubmed
Manley,
Polyadenylation of mRNA precursors.
1988,
Pubmed
Needleman,
A general method applicable to the search for similarities in the amino acid sequence of two proteins.
1970,
Pubmed
Neyton,
Discrete Ba2+ block as a probe of ion occupancy and pore structure in the high-conductance Ca2+ -activated K+ channel.
1988,
Pubmed
Papazian,
Cloning of genomic and complementary DNA from Shaker, a putative potassium channel gene from Drosophila.
1987,
Pubmed
Pardo,
Extracellular K+ specifically modulates a rat brain K+ channel.
1992,
Pubmed
,
Xenbase
Pongs,
Shaker encodes a family of putative potassium channel proteins in the nervous system of Drosophila.
1988,
Pubmed
RITCHIE,
The hyperpolarization which follows activity in mammalian non-medullated fibres.
1957,
Pubmed
Salkoff,
Surfing the DNA databases for K+ channels nets yet more diversity.
1995,
Pubmed
Schachtman,
Structure and transport mechanism of a high-affinity potassium uptake transporter from higher plants.
1994,
Pubmed
,
Xenbase
Sentenac,
Cloning and expression in yeast of a plant potassium ion transport system.
1992,
Pubmed
Shapiro,
Permeant ion effects on the gating kinetics of the type L potassium channel in mouse lymphocytes.
1991,
Pubmed
Silver,
Effects of external Rb+ on inward rectifier K+ channels of bovine pulmonary artery endothelial cells.
1994,
Pubmed
Strong,
Molecular evolution of voltage-sensitive ion channel genes: on the origins of electrical excitability.
1993,
Pubmed
Swenson,
K+ channels close more slowly in the presence of external K+ and Rb+.
1981,
Pubmed
Vandenberg,
Inward rectification of a potassium channel in cardiac ventricular cells depends on internal magnesium ions.
1987,
Pubmed
Wang,
MinK residues line a potassium channel pore.
1996,
Pubmed
Wang,
Subunit composition of minK potassium channels.
1995,
Pubmed
,
Xenbase
Wei,
K+ current diversity is produced by an extended gene family conserved in Drosophila and mouse.
1990,
Pubmed
,
Xenbase
Wilson,
2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans.
1994,
Pubmed
Woodhull,
Ionic blockage of sodium channels in nerve.
1973,
Pubmed
Yang,
Determination of the subunit stoichiometry of an inwardly rectifying potassium channel.
1995,
Pubmed
,
Xenbase
Yasuda,
Cloning of a novel somatostatin receptor, SSTR3, coupled to adenylylcyclase.
1992,
Pubmed
Zhou,
YKC1 encodes the depolarization-activated K+ channel in the plasma membrane of yeast.
1995,
Pubmed
