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Mol Cell Biol
1996 Oct 01;1610:5572-8. doi: 10.1128/MCB.16.10.5572.
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Upstream binding factor stabilizes Rib 1, the TATA-binding-protein-containing Xenopus laevis RNA polymerase I transcription factor, by multiple protein interactions in a DNA-independent manner.
Bodeker M
,
Cairns C
,
McStay B
.
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Initiation of RNA polymerase I transcription in Xenopus laevis requires Rib 1 and upstream binding factor (UBF). UBF and Rib 1 combine to form a stable transcription complex on the Xenopus ribosomal gene promoter. Here we show that Rib 1 comprises TATA-binding protein (TBP) and TBP-associated factor components. Thus, Rib 1 is the Xenopus equivalent of mammalian SL 1. In contrast to SL 1, Rib 1 is an unstable complex that readily dissociates into TBP and associated components. We identify a novel function for UBF in stabilizing Rib 1 by multiple protein interactions. This stabilization occurs in solution in a DNA-independent manner. These results may partially explain the difference in UBF requirement between Xenopus and mammalian systems.
Bachvarov,
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Bachvarov,
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,
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Beckmann,
Coactivator and promoter-selective properties of RNA polymerase I TAFs.
1995,
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Bell,
Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis.
1988,
Pubmed
Cairns,
HMG box 4 is the principal determinant of species specificity in the RNA polymerase I transcription factor UBF.
1995,
Pubmed
,
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Chan,
Human autoantibody to RNA polymerase I transcription factor hUBF. Molecular identity of nucleolus organizer region autoantigen NOR-90 and ribosomal RNA transcription upstream binding factor.
1991,
Pubmed
Comai,
Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits.
1994,
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Comai,
The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1.
1992,
Pubmed
Eberhard,
A TBP-containing multiprotein complex (TIF-IB) mediates transcription specificity of murine RNA polymerase I.
1993,
Pubmed
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Field,
Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
1988,
Pubmed
Hashimoto,
Conserved structural motifs within the N-terminal domain of TFIID tau from Xenopus, mouse and human.
1992,
Pubmed
,
Xenbase
Hempel,
The species-specific RNA polymerase I transcription factor SL-1 binds to upstream binding factor.
1996,
Pubmed
Hisatake,
Cloning and structural analysis of cDNA and the gene for mouse transcription factor UBF.
1991,
Pubmed
Hu,
xUBF, an RNA polymerase I transcription factor, binds crossover DNA with low sequence specificity.
1994,
Pubmed
,
Xenbase
Jantzen,
Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins.
1990,
Pubmed
Kuhn,
The nucleolar transcription activator UBF relieves Ku antigen-mediated repression of mouse ribosomal gene transcription.
1993,
Pubmed
Kuhn,
Dual role of the nucleolar transcription factor UBF: trans-activator and antirepressor.
1992,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Kwon,
The RNA polymerase I transcription factor, upstream binding factor, interacts directly with the TATA box-binding protein.
1994,
Pubmed
Learned,
Purification and characterization of a transcription factor that confers promoter specificity to human RNA polymerase I.
1985,
Pubmed
McStay,
xUBF contains a novel dimerization domain essential for RNA polymerase I transcription.
1991,
Pubmed
,
Xenbase
McStay,
An RNA polymerase I termination site can stimulate the adjacent ribosomal gene promoter by two distinct mechanisms in Xenopus laevis.
1990,
Pubmed
,
Xenbase
McStay,
xUBF and Rib 1 are both required for formation of a stable polymerase I promoter complex in X. laevis.
1991,
Pubmed
,
Xenbase
O'Mahony,
Identification of two forms of the RNA polymerase I transcription factor UBF.
1991,
Pubmed
Pikaard,
The Xenopus ribosomal gene enhancers bind an essential polymerase I transcription factor, xUBF.
1989,
Pubmed
,
Xenbase
Rigby,
Three in one and one in three: it all depends on TBP.
1993,
Pubmed
Rudloff,
The conserved core domain of the human TATA binding protein is sufficient to assemble the multisubunit RNA polymerase I-specific transcription factor SL1.
1994,
Pubmed
Smith,
Transcription from the rat 45S ribosomal DNA promoter does not require the factor UBF.
1993,
Pubmed
Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed
Voit,
The nucleolar transcription factor mUBF is phosphorylated by casein kinase II in the C-terminal hyperacidic tail which is essential for transactivation.
1992,
Pubmed
Zomerdijk,
Assembly of transcriptionally active RNA polymerase I initiation factor SL1 from recombinant subunits.
1994,
Pubmed
