Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Blockade of swelling-induced chloride channels by phenol derivatives.
Gschwentner M
,
Jungwirth A
,
Hofer S
,
Wöll E
,
Ritter M
,
Susanna A
,
Schmarda A
,
Reibnegger G
,
Pinggera GM
,
Leitinger M
,
Frick J
,
Deetjen P
,
Paulmichl M
.
???displayArticle.abstract???
1. In NIH3T3 fibroblasts, the chloride channel involved in regulatory volume decrease (RVD) was identified as ICln, a protein isolated from a cDNA library derived from Madin Darby canine Kidney (MDCK) cells. ICln expressed in Xenopus laevis oocytes gives rise to an outwardly rectifying chloride current, sensitive to the extracellular addition of nucleotides and the known chloride channel blockers, DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid) and NPPB (5-nitro-2-(3-phenylpropylamino)-benzoic acid). We set out to study whether substances structurally similar to NPPB are able to interfere with RVD. 2. RVD in NIH3T3 fibroblasts and MDCK cells is temperature-dependent. 3. RVD, the swelling-dependent chloride current and the depolarization seen after reducing extracellular osmolarity can be blocked by gossypol and NDGA (nordihydroguaiaretic acid), both structurally related to NPPB. 4. The cyclic AMP-dependent chloride current elicited in CaCo cells is less sensitive to the two substances tested while the calcium-activated chloride current in fibroblasts is insensitive. 5. The binding site for the two phenol derivatives onto ICln seems to be distinct but closely related to the nucleotide binding site identified as G x G x G, a glycine repeat located at the predicted outer mouth of the ICln channel protein.
Biwersi,
Cell-permeable fluorescent indicator for cytosolic chloride.
1991, Pubmed
Biwersi,
Cell-permeable fluorescent indicator for cytosolic chloride.
1991,
Pubmed
Bubien,
Cell cycle dependence of chloride permeability in normal and cystic fibrosis lymphocytes.
1990,
Pubmed
Cantiello,
G alpha i-3 regulates epithelial Na+ channels by activation of phospholipase A2 and lipoxygenase pathways.
1990,
Pubmed
,
Xenbase
Doroshenko,
Second messengers mediating activation of chloride current by intracellular GTP gamma S in bovine chromaffin cells.
1991,
Pubmed
Gekle,
Ochratoxin A impairs "postproximal" nephron function in vivo and blocks plasma membrane anion conductance in Madin-Darby canine kidney cells in vitro.
1993,
Pubmed
Gschwentner,
Structure-function relation of a cloned epithelial chloride channel.
1994,
Pubmed
,
Xenbase
Gschwentner,
Antisense oligonucleotides suppress cell-volume-induced activation of chloride channels.
1995,
Pubmed
Gschwentner,
Antiviral drugs from the nucleoside analog family block volume-activated chloride channels.
1995,
Pubmed
Hamasaki,
Gossypol, a potent inhibitor of arachidonate 5- and 12-lipoxygenases.
1985,
Pubmed
Kim,
G-protein beta gamma-subunits activate the cardiac muscarinic K+-channel via phospholipase A2.
1989,
Pubmed
Krapivinsky,
Molecular characterization of a swelling-induced chloride conductance regulatory protein, pICln.
1994,
Pubmed
,
Xenbase
Kunzelmann,
Properties and regulation of chloride channels in cystic fibrosis and normal airway cells.
1989,
Pubmed
Kurachi,
Arachidonic acid metabolites as intracellular modulators of the G protein-gated cardiac K+ channel.
1989,
Pubmed
Lambert,
Role of prostaglandins and leukotrienes in volume regulation by Ehrlich ascites tumor cells.
1987,
Pubmed
Lang,
The biological significance of cell volume.
1993,
Pubmed
Lang,
Apparent chloride conductance of subconfluent Madin Darby canine kidney cells.
1986,
Pubmed
Mastrocola,
Characterization of chloride transport pathways in cultured human keratinocytes.
1991,
Pubmed
McManus,
Ketoconazole blocks organic osmolyte efflux independently of its effect on arachidonic acid conversion.
1994,
Pubmed
Mochizuki,
Arachidonic acid inhibits Cl secretion in cultures of dog tracheal epithelium.
1992,
Pubmed
Oberleithner,
Anthracene-9-carboxylic acid inhibits renal chloride reabsorption.
1983,
Pubmed
Palade,
On the inhibition of muscle membrane chloride conductance by aromatic carboxylic acids.
1977,
Pubmed
Paulmichl,
The effect of hypoosmolarity on the electrical properties of Madin Darby canine kidney cells.
1989,
Pubmed
Paulmichl,
New mammalian chloride channel identified by expression cloning.
1992,
Pubmed
,
Xenbase
Paulmichl,
Enhancement of intracellular calcium concentration by extracellular ATP and UTP in Madin Darby Canine Kidney cells.
1988,
Pubmed
Peppelenbosch,
Epidermal growth factor activates calcium channels by phospholipase A2/5-lipoxygenase-mediated leukotriene C4 production.
1992,
Pubmed
Piomelli,
Lipoxygenase metabolites of arachidonic acid as second messengers for presynaptic inhibition of Aplysia sensory cells.
,
Pubmed
Sgaragli,
Calcium antagonist and antiperoxidant properties of some hindered phenols.
1993,
Pubmed
Strange,
Mechanism and regulation of swelling-activated inositol efflux in brain glial cells.
1993,
Pubmed
Strange,
Swelling-activated organic osmolyte efflux: a new role for anion channels.
1995,
Pubmed
Tien,
Activation of the cystic fibrosis transmembrane conductance regulator by cGMP in the human colonic cancer cell line, Caco-2.
1994,
Pubmed
Verkman,
Development and biological applications of chloride-sensitive fluorescent indicators.
1990,
Pubmed
Wangemann,
Cl(-)-channel blockers in the thick ascending limb of the loop of Henle. Structure activity relationship.
1986,
Pubmed
Weiss,
Ion channels activated by swelling of Madin Darby canine kidney (MDCK) cells.
1992,
Pubmed
Wöll,
The role of calcium in cell shrinkage and intracellular alkalinization by bradykinin in Ha-ras oncogene expressing cells.
1993,
Pubmed