Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Assembly, nuclear import and function of U7 snRNPs studied by microinjection of synthetic U7 RNA into Xenopus oocytes.
Stefanovic B
,
Hackl W
,
Lührmann R
,
Schümperli D
.
???displayArticle.abstract???
In Xenopus oocytes in vitro transcribed mouse U7 RNA is assembled into small nuclear ribonucleoproteins (snRNPs) that are functional in histone RNA 3' processing. If the special Sm binding site of U7 (AAUUUGUCUAG, U7 Sm WT) is converted into the canonical Sm sequence derived from the major snRNAs (AAUUUUUGGAG, U7 Sm OPT) the RNA assembles into a particle which accumulates more efficiently in the nucleus, but which is non-functional. U7 RNA with a heavily mutated Sm binding site (AACGCGUCAUG, U7 Sm MUT) is deficient in nuclear accumulation and function. By UV cross-linking U7 Sm WT RNA can be linked to three proteins, i.e. the common snRNP proteins G and B/B' and an apparently U7-specific protein of 40 kDa. As a result of altering the Sm binding site, U7 Sm OPT RNA cannot be cross-linked to the 40 kDa protein and no cross-links are obtained with U7 Sm MUT RNA. The fact that the Sm site also interacts with at least one U7-specific protein is so far unique to U7 RNA and may provide an explanation for the atypical sequence of this site. All described RNA-protein interactions, including that with the 40 kDa protein, already occur in the cytoplasm. An additional cytoplasmic photoadduct obtained with U7 Sm WT and U7 Sm OPT, but not U7 Sm MUT, RNAs is indicative of a protein of 60-80 kDa. The m7G cap structure of U7 Sm WT and U7 Sm OPT RNA becomes hypermethylated. However, the 3mG cap enhances, but is not required for, nuclear accumulation. Finally, U7 Sm WT RNA is functional in histone RNA processing even when bearing an ApppG cap.
Bond,
Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro.
1991, Pubmed
Bond,
Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro.
1991,
Pubmed
Cazenave,
Rate of degradation of [alpha]- and [beta]-oligodeoxynucleotides in Xenopus oocytes. Implications for anti-messenger strategies.
1987,
Pubmed
,
Xenbase
De Lorenzi,
Analysis of a sea urchin gene cluster coding for the small nuclear U7 RNA, a rare RNA species implicated in the 3' editing of histone precursor mRNAs.
1986,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
England,
3'-terminal labelling of RNA with T4 RNA ligase.
1978,
Pubmed
Feeney,
Nuclear exchange of the U1 and U2 snRNP-specific proteins.
1990,
Pubmed
Fischer,
Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport.
1991,
Pubmed
,
Xenbase
Fischer,
Nuclear transport of U1 snRNP in somatic cells: differences in signal requirement compared with Xenopus laevis oocytes.
1994,
Pubmed
,
Xenbase
Fischer,
An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus.
1990,
Pubmed
,
Xenbase
Fisher,
Small nuclear ribonucleoprotein particle assembly in vivo: demonstration of a 6S RNA-free core precursor and posttranslational modification.
1985,
Pubmed
Galli,
Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
1983,
Pubmed
,
Xenbase
Gilmartin,
Functional analysis of the sea urchin U7 small nuclear RNA.
1988,
Pubmed
,
Xenbase
Grimm,
The low abundance of U7 snRNA is partly determined by its Sm binding site.
1993,
Pubmed
,
Xenbase
Gruber,
Isolation of an active gene and of two pseudogenes for mouse U7 small nuclear RNA.
1991,
Pubmed
,
Xenbase
Hamm,
The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal.
1990,
Pubmed
,
Xenbase
Hamm,
Functional analysis of mutant Xenopus U2 snRNAs.
1989,
Pubmed
,
Xenbase
Heinrichs,
Direct binding of small nuclear ribonucleoprotein G to the Sm site of small nuclear RNA. Ultraviolet light cross-linking of protein G to the AAU stretch within the Sm site (AAUUUGUGG) of U1 small nuclear ribonucleoprotein reconstituted in vitro.
1992,
Pubmed
Jarmolowski,
The determinants for Sm protein binding to Xenopus U1 and U5 snRNAs are complex and non-identical.
1993,
Pubmed
,
Xenbase
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1987,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase
Luhrmann,
Isolation and characterization of rabbit anti-m3 2,2,7G antibodies.
1982,
Pubmed
Lührmann,
Structure of spliceosomal snRNPs and their role in pre-mRNA splicing.
1990,
Pubmed
Lund,
In vitro synthesis of vertebrate U1 snRNA.
1989,
Pubmed
,
Xenbase
Lutz-Freyermuth,
The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs.
1989,
Pubmed
Marshallsay,
In vitro nuclear import of snRNPs: cytosolic factors mediate m3G-cap dependence of U1 and U2 snRNP transport.
1994,
Pubmed
,
Xenbase
Mattaj,
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
1986,
Pubmed
,
Xenbase
Melin,
Biochemical demonstration of complex formation of histone pre-mRNA with U7 small nuclear ribonucleoprotein and hairpin binding factors.
1992,
Pubmed
Michaud,
Microinjected U snRNAs are imported to oocyte nuclei via the nuclear pore complex by three distinguishable targeting pathways.
1992,
Pubmed
,
Xenbase
Mital,
Detection of UV-induced RNA:protein crosslinks in snRNPs by oligonucleotides complementary to the snRNA.
1993,
Pubmed
Mowry,
Identification of the human U7 snRNP as one of several factors involved in the 3' end maturation of histone premessenger RNA's.
1987,
Pubmed
Nigg,
Nuclear import-export: in search of signals and mechanisms.
1991,
Pubmed
Pan,
Assembly of functional U1 and U2 human-amphibian hybrid snRNPs in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Patton,
U1 small nuclear ribonucleoprotein particle-specific proteins interact with the first and second stem-loops of U1 RNA, with the A protein binding directly to the RNA independently of the 70K and Sm proteins.
1989,
Pubmed
Peculis,
Sequence and structural elements critical for U8 snRNP function in Xenopus oocytes are evolutionarily conserved.
1994,
Pubmed
,
Xenbase
Phillips,
Nucleotide sequence of the mouse U7 snRNA gene.
1991,
Pubmed
Phillips,
Analysis of a gene cluster coding for the Xenopus laevis U7 snRNA.
1992,
Pubmed
,
Xenbase
Query,
A specific 31-nucleotide domain of U1 RNA directly interacts with the 70K small nuclear ribonucleoprotein component.
1989,
Pubmed
Reuter,
Immunization of mice with purified U1 small nuclear ribonucleoprotein (RNP) induces a pattern of antibody specificities characteristic of the anti-Sm and anti-RNP autoimmune response of patients with lupus erythematosus, as measured by monoclonal antibodies.
1986,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schaufele,
Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
,
Pubmed
,
Xenbase
Smith,
Two-step affinity purification of U7 small nuclear ribonucleoprotein particles using complementary biotinylated 2'-O-methyl oligoribonucleotides.
1991,
Pubmed
Soldati,
Structural and functional characterization of mouse U7 small nuclear RNA active in 3' processing of histone pre-mRNA.
1988,
Pubmed
Stefanovic,
A synthetic histone pre-mRNA-U7 small nuclear RNA chimera undergoing cis cleavage in the cytoplasm of Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Streit,
Variable effects of the conserved RNA hairpin element upon 3' end processing of histone pre-mRNA in vitro.
1993,
Pubmed
Strub,
Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte.
1986,
Pubmed
,
Xenbase
Surowy,
Direct, sequence-specific binding of the human U1-70K ribonucleoprotein antigen protein to loop I of U1 small nuclear RNA.
1989,
Pubmed
