Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1995 Aug 01;1415:3777-87. doi: 10.1002/j.1460-2075.1995.tb00047.x.
Show Gene links
Show Anatomy links
Staf, a novel zinc finger protein that activates the RNA polymerase III promoter of the selenocysteine tRNA gene.
Schuster C
,
Myslinski E
,
Krol A
,
Carbon P
.
???displayArticle.abstract???
The selenocysteine tRNA gene (tRNA(Sec)) is atypical. Though transcribed by RNA polymerase III like all other tRNA genes, its basal promoter elements are distinct and reside essentially upstream of the coding region. In addition, transcription from the basal promoter is activated by a 15 bp activator element. In this report we describe the cloning and functional characterization of Staf (selenocysteine tRNA gene transcription activating factor), a novel Xenopus laevis transcription factor which binds to the tRNA(Sec) activator element and mediates its activation properties. The 600 amino acid Staf protein contains seven zinc fingers and a separate acidic activation domain. Seven highly conserved regions were detected between Staf and human ZNF76, a protein of unknown function, thereby aiding in predicting the locations of the functional domains of Staf. With the use of a novel expression assay in X.laevis oocytes we succeeded in demonstrating that Staf can activate the RNA polymerase III promoter of the tRNA(Sec) gene. This constitutes the first demonstration of the capacity of a cloned factor to activate RNA polymerase III transcription in vivo.
Behne,
Evidence for specific selenium target tissues and new biologically important selenoproteins.
1988, Pubmed
Behne,
Evidence for specific selenium target tissues and new biologically important selenoproteins.
1988,
Pubmed
Bellefroid,
The evolutionarily conserved Krüppel-associated box domain defines a subfamily of eukaryotic multifingered proteins.
1991,
Pubmed
,
Xenbase
Bradley,
The structure and expression of the Xenopus Krox-20 gene: conserved and divergent patterns of expression in rhombomeres and neural crest.
1993,
Pubmed
,
Xenbase
Brown,
The primary structure of transcription factor TFIIIA has 12 consecutive repeats.
1985,
Pubmed
,
Xenbase
Caput,
Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators.
1986,
Pubmed
Carbon,
A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.
1987,
Pubmed
,
Xenbase
Carbon,
Transcription of the Xenopus laevis selenocysteine tRNA(Ser)Sec gene: a system that combines an internal B box and upstream elements also found in U6 snRNA genes.
1991,
Pubmed
,
Xenbase
Carey,
Mechanistic advances in eukaryotic gene activation.
1991,
Pubmed
Chavrier,
Structure, chromosome location, and expression of the mouse zinc finger gene Krox-20: multiple gene products and coregulation with the proto-oncogene c-fos.
1989,
Pubmed
Chavrier,
The segment-specific gene Krox-20 encodes a transcription factor with binding sites in the promoter region of the Hox-1.4 gene.
1990,
Pubmed
Courey,
Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif.
1988,
Pubmed
Danzeiser,
Functional characterization of elements in a human U6 small nuclear RNA gene distal control region.
1993,
Pubmed
Delwel,
Four of the seven zinc fingers of the Evi-1 myeloid-transforming gene are required for sequence-specific binding to GA(C/T)AAGA(T/C)AAGATAA.
1993,
Pubmed
Gabrielsen,
RNA polymerase III (C) and its transcription factors.
1991,
Pubmed
,
Xenbase
Green,
A versatile in vivo and in vitro eukaryotic expression vector for protein engineering.
1988,
Pubmed
Herbomel,
Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma.
1984,
Pubmed
Keller,
Only two of the five zinc fingers of the eukaryotic transcriptional repressor PRDI-BF1 are required for sequence-specific DNA binding.
1992,
Pubmed
Kleinert,
Expression of a human 7S K RNA gene in vivo requires a novel pol III upstream element.
1990,
Pubmed
Klocke,
The FAR domain defines a new Xenopus laevis zinc finger protein subfamily with specific RNA homopolymer binding activity.
1994,
Pubmed
,
Xenbase
Knöchel,
Evolutionary conserved modules associated with zinc fingers in Xenopus laevis.
1989,
Pubmed
,
Xenbase
Krasnow,
Transcriptional activation and repression by Ultrabithorax proteins in cultured Drosophila cells.
1989,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Krol,
Xenopus tropicalis U6 snRNA genes transcribed by Pol III contain the upstream promoter elements used by Pol II dependent U snRNA genes.
1987,
Pubmed
,
Xenbase
Kunkel,
Upstream elements required for efficient transcription of a human U6 RNA gene resemble those of U1 and U2 genes even though a different polymerase is used.
1988,
Pubmed
Kunkel,
RNA polymerase III transcription of genes that lack internal control regions.
1991,
Pubmed
,
Xenbase
Lemaire,
Expression cloning of Siamois, a Xenopus homeobox gene expressed in dorsal-vegetal cells of blastulae and able to induce a complete secondary axis.
1995,
Pubmed
,
Xenbase
Lescure,
A factor with Sp1 DNA-binding specificity stimulates Xenopus U6 snRNA in vivo transcription by RNA polymerase III.
1992,
Pubmed
,
Xenbase
Lescure,
The proximal promoter and the start site cooperate to specify correct U1 snRNA transcription initiation by RNA polymerase II.
1992,
Pubmed
,
Xenbase
Lobo,
A 7 bp mutation converts a human RNA polymerase II snRNA promoter into an RNA polymerase III promoter.
1989,
Pubmed
Matheny,
The nuclear localization signal of NGFI-A is located within the zinc finger DNA binding domain.
1994,
Pubmed
Mattaj,
Changing the RNA polymerase specificity of U snRNA gene promoters.
1988,
Pubmed
,
Xenbase
Meissner,
Transcription factors required for the expression of Xenopus laevis selenocysteine tRNA in vitro.
1994,
Pubmed
,
Xenbase
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Mitchell,
Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
1989,
Pubmed
Murphy,
Purified octamer binding transcription factors stimulate RNA polymerase III--mediated transcription of the 7SK RNA gene.
1989,
Pubmed
Murphy,
Oct-1 and Oct-2 potentiate functional interactions of a transcription factor with the proximal sequence element of small nuclear RNA genes.
1992,
Pubmed
Myslinski,
Promoter strength and structure dictate module composition in RNA polymerase III transcriptional activator elements.
1993,
Pubmed
,
Xenbase
Myslinski,
Optimal tRNA((Ser)Sec) gene activity requires an upstream SPH motif.
1992,
Pubmed
,
Xenbase
Myslinski,
Point mutations 5' to the tRNA selenocysteine TATA box alter RNA polymerase III transcription by affecting the binding of TBP.
1993,
Pubmed
,
Xenbase
Numoto,
Transcriptional repressor ZF5 identifies a new conserved domain in zinc finger proteins.
1993,
Pubmed
Pavletich,
Crystal structure of a five-finger GLI-DNA complex: new perspectives on zinc fingers.
1993,
Pubmed
Pazin,
ATP-dependent nucleosome reconfiguration and transcriptional activation from preassembled chromatin templates.
1994,
Pubmed
Perkins,
An interaction between the DNA-binding domains of RelA(p65) and Sp1 mediates human immunodeficiency virus gene activation.
1994,
Pubmed
Ragoussis,
A testis-expressed Zn finger gene (ZNF76) in human 6p21.3 centromeric to the MHC is closely linked to the human homolog of the t-complex gene tcp-11.
1992,
Pubmed
Simmen,
TFIID is required for in vitro transcription of the human U6 gene by RNA polymerase III.
1991,
Pubmed
Singh,
Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA.
1988,
Pubmed
Stadtman,
Biosynthesis and function of selenocysteine-containing enzymes.
1991,
Pubmed
Staudt,
Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif.
1988,
Pubmed
Sturm,
The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
1988,
Pubmed
Tanaka,
Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter.
1992,
Pubmed
Thisse,
Sequence-specific transactivation of the Drosophila twist gene by the dorsal gene product.
1991,
Pubmed
Tjian,
Transcriptional activation: a complex puzzle with few easy pieces.
1994,
Pubmed
Vesque,
Mapping functional regions of the segment-specific transcription factor Krox-20.
1992,
Pubmed
Vinson,
In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
1988,
Pubmed
Waldschmidt,
Identification of transcription factors required for the expression of mammalian U6 genes in vitro.
1991,
Pubmed
Webster,
The yeast UASG is a transcriptional enhancer in human HeLa cells in the presence of the GAL4 trans-activator.
1988,
Pubmed
Willis,
RNA polymerase III. Genes, factors and transcriptional specificity.
1993,
Pubmed