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XB-ART-19594
Proc Natl Acad Sci U S A 1995 Jun 20;9213:6092-6. doi: 10.1073/pnas.92.13.6092.
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A corticosteroid-induced gene expressing an "IsK-like" K+ channel activity in Xenopus oocytes.

Attali B , Latter H , Rachamim N , Garty H .


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Screening a rat colon cDNA library for aldosterone-induced genes resulted in the molecular cloning of a cDNA whose corresponding mRNA is strongly induced in the colon by dexamethasone, aldosterone, and a low NaCl diet. A similar mRNA was detected in kidney papilla but not in brain, heart, or skeletal muscle. Xenopus laevis oocytes injected with cRNA synthesized from this clone, designated CHIF (channel-inducing factor), express a K(+)-specific channel activity. The biophysical, pharmacological, and regulatory characteristics of this channel are very similar to those reported before for IsK (minK). These include: slow (tau > 20 s) activation by membrane depolarization with a threshold potential above -50 mV, blockade by clofilium, inhibition by phorbol ester, and activation by 8-bromoadenosine 3',5'-cyclic monophosphate and high cytoplasmic Ca2+. The primary structure of this clone, however, shows no homology to IsK. Instead, CHIF exhibits > 50% similarity to two other short bitopic membrane proteins, phospholemman and the gamma subunit of Na+K(+)-ATPase. The data are consistent with the possibility that CHIF is a member of a family of transmembrane regulators capable of activating endogenous oocyte transport proteins.

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Species referenced: Xenopus laevis
Genes referenced: fxyd1 kcne1

References [+] :
Asher, Aldosterone increases the apical Na+ permeability of toad bladder by two different mechanisms. 1988, Pubmed