Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1995 Jun 01;1411:2409-16. doi: 10.1002/j.1460-2075.1995.tb07238.x.
Show Gene links
Show Anatomy links
Cloning and electrophysiological analysis of KST1, an inward rectifying K+ channel expressed in potato guard cells.
Müller-Röber B
,
Ellenberg J
,
Provart N
,
Willmitzer L
,
Busch H
,
Becker D
,
Dietrich P
,
Hoth S
,
Hedrich R
.
???displayArticle.abstract???
Potassium uptake by guard cells represents part of the osmotic motor which drives stomatal opening. Patch-clamp measurements have identified inward rectifying K+ channels capable of mediating K+ uptake in guard cells and various other plant cell types. Here we report the molecular cloning and characterization of a voltage-dependent K+ channel (KST1) from potato (Solanum tuberosum L.) guard cells. In situ hybridization shows expression of kst1 in guard cells. Two-electrode voltage-clamp and patch-clamp studies of the gene product after cRNA injection into Xenopus oocytes identified KST1 as a slowly activating, voltage-dependent, inward rectifying K+ channel. The single channel current voltage curve was linear in the range -160 to +20 mV, with a deduced single channel conductance of 7 pS in symmetrical 100 mM K+. This channel type, modulated by pH changes within the physiological range, required ATP for activation. In line with the properties of a K(+)-selective channel, KST1 was permeable to K+, Rb+ and NH4+ and excluded Na+ and Li+. Cs+ at submillimolar concentrations blocked the channel in a voltage-dependent manner. Related studies on potato guard cell protoplasts confirmed the biophysical characteristics of the kst1 gene product (KST1) in the heterologous expression system. Therefore, KST1 represents a major K+ uptake channel in potato guard cells.
Aldrich,
Potassium channels. Advent of a new family.
1993, Pubmed,
Xenbase
Aldrich,
Potassium channels. Advent of a new family.
1993,
Pubmed
,
Xenbase
Amasino,
Acceleration of nucleic acid hybridization rate by polyethylene glycol.
1986,
Pubmed
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992,
Pubmed
Barry,
Liquid junction potentials and small cell effects in patch-clamp analysis.
1991,
Pubmed
Blatt,
K+ channels of stomatal guard cells. Characteristics of the inward rectifier and its control by pH.
1992,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Hedrich,
Ca2+ and nucleotide dependent regulation of voltage dependent anion channels in the plasma membrane of guard cells.
1990,
Pubmed
Hedrich,
Green circuits--the potential of plant specific ion channels.
1994,
Pubmed
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Jan,
Structural elements involved in specific K+ channel functions.
1992,
Pubmed
Kubo,
Primary structure and functional expression of a mouse inward rectifier potassium channel.
1993,
Pubmed
,
Xenbase
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Milkman,
An Escherichia coli homologue of eukaryotic potassium channel proteins.
1994,
Pubmed
Neher,
Correction for liquid junction potentials in patch clamp experiments.
1992,
Pubmed
Papazian,
Cloning of genomic and complementary DNA from Shaker, a putative potassium channel gene from Drosophila.
1987,
Pubmed
Pearson,
Improved tools for biological sequence comparison.
1988,
Pubmed
Saraste,
The P-loop--a common motif in ATP- and GTP-binding proteins.
1990,
Pubmed
Schachtman,
Structure and transport mechanism of a high-affinity potassium uptake transporter from higher plants.
1994,
Pubmed
,
Xenbase
Schmelzer,
Temporal and Spatial Patterns of Gene Expression around Sites of Attempted Fungal Infection in Parsley Leaves.
1989,
Pubmed
Sentenac,
Cloning and expression in yeast of a plant potassium ion transport system.
1992,
Pubmed
Siddik,
Flameless atomic absorption spectrophotometric determination of platinum in tissues solubilized in hyamine hydroxide.
1987,
Pubmed
Somssich,
Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsley.
1988,
Pubmed
Spalding,
Activation of K+ Channels in the Plasma Membrane of Arabidopsis by ATP Produced Photosynthetically.
1993,
Pubmed
Stühmer,
Patch clamp characterization of sodium channels expressed from rat brain cDNA.
1987,
Pubmed
,
Xenbase
