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Protein prenylation is a posttranslational modification involving the covalent attachment of a prenyl lipid to a cysteine at or near the COOH terminus of a protein. It is required for membrane localization and efficient function of a number of cytoplasmic as well as nuclear proteins including the proto-oncogenic and activated forms of Ras. Farnesylation in conjunction with a nuclear localization signal has been shown to be necessary to target newly synthesized nuclear lamins to the inner nuclear envelope membrane. It is, however, not clear where in the cell isoprenylation of nuclear lamins takes place. In this study we describe in vivo and in vitro experiments on the isoprenylation of the Xenopus oocyte nuclear lamin B3. We show by kinetic analysis that newly synthesized lamins are isoprenylated in the cytosol of oocytes before uptake into the nucleus. From our data it can be concluded that isoprenylation of lamins in the nucleus, as it is observed under certain conditions of isoprene starvation, represents a default pathway rather than the physiological situation. We further analyzed the capacity of isolated nuclei to carry out isoprenylation of B3. Our results are in line with a dual localization of a protein farnesyltransferase in the cytosol and nuclei of amphibian oocytes. Implications for the possible functions of a nuclear protein farnesyltransferase as well as possible mechanisms of the selective inhibition of farnesylation of cytoplasmic proteins by peptidomimetics are discussed.
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
, Pubmed,
Xenbase
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
,
Pubmed
,
Xenbase
Bailer,
Characterization of A 54-kD protein of the inner nuclear membrane: evidence for cell cycle-dependent interaction with the nuclear lamina.
1991,
Pubmed
Beck,
Isoprenylation is required for the processing of the lamin A precursor.
1990,
Pubmed
Black,
Development of hydrophobicity parameters for prenylated proteins.
1992,
Pubmed
Borer,
Major nucleolar proteins shuttle between nucleus and cytoplasm.
1989,
Pubmed
,
Xenbase
Brown,
Protein prenylation. Mad bet for Rab.
1993,
Pubmed
Butrynski,
Differential isoprenylation of carboxy-terminal mutants of an inhibitory G-protein alpha-subunit: neither farnesylation nor geranylgeranylation is sufficient for membrane attachment.
1992,
Pubmed
Casey,
p21ras is modified by a farnesyl isoprenoid.
1989,
Pubmed
Clarke,
Protein isoprenylation and methylation at carboxyl-terminal cysteine residues.
1992,
Pubmed
Cox,
Protein prenylation: more than just glue?
1992,
Pubmed
Dingwall,
Nuclear targeting sequences--a consensus?
1991,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Firmbach-Kraft,
The role of CaaX-dependent modifications in membrane association of Xenopus nuclear lamin B3 during meiosis and the fate of B3 in transfected mitotic cells.
1993,
Pubmed
,
Xenbase
Foisner,
Integral membrane proteins of the nuclear envelope interact with lamins and chromosomes, and binding is modulated by mitotic phosphorylation.
1993,
Pubmed
Fukada,
Farnesylated gamma-subunit of photoreceptor G protein indispensable for GTP-binding.
1990,
Pubmed
Gerace,
Nuclear lamina and the structural organization of the nuclear envelope.
1982,
Pubmed
Hancock,
All ras proteins are polyisoprenylated but only some are palmitoylated.
1989,
Pubmed
Hancock,
Methylation and proteolysis are essential for efficient membrane binding of prenylated p21K-ras(B).
1991,
Pubmed
Hancock,
A polybasic domain or palmitoylation is required in addition to the CAAX motif to localize p21ras to the plasma membrane.
1990,
Pubmed
Hennekes,
The role of isoprenylation in membrane attachment of nuclear lamins. A single point mutation prevents proteolytic cleavage of the lamin A precursor and confers membrane binding properties.
1994,
Pubmed
Holtz,
The CaaX motif of lamin A functions in conjunction with the nuclear localization signal to target assembly to the nuclear envelope.
1989,
Pubmed
Itoh,
The post-translational processing of ras p21 is critical for its stimulation of mitogen-activated protein kinase.
1993,
Pubmed
,
Xenbase
James,
Benzodiazepine peptidomimetics: potent inhibitors of Ras farnesylation in animal cells.
1993,
Pubmed
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Kita,
Feedback regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in livers of mice treated with mevinolin, a competitive inhibitor of the reductase.
1980,
Pubmed
Kitten,
The CaaX motif is required for isoprenylation, carboxyl methylation, and nuclear membrane association of lamin B2.
1991,
Pubmed
Krohne,
The conserved carboxy-terminal cysteine of nuclear lamins is essential for lamin association with the nuclear envelope.
1989,
Pubmed
,
Xenbase
Krohne,
Cell type-specific differences in protein composition of nuclear pore complex-lamina structures in oocytes and erythrocytes of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Kuroda,
The effect of posttranslational modifications on the interaction of Ras2 with adenylyl cyclase.
1993,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Loewinger,
Mutations in the nuclear lamin proteins resulting in their aberrant assembly in the cytoplasm.
1988,
Pubmed
Lourim,
Membrane-associated lamins in Xenopus egg extracts: identification of two vesicle populations.
1993,
Pubmed
,
Xenbase
Lutz,
Nucleoplasmic localization of prelamin A: implications for prenylation-dependent lamin A assembly into the nuclear lamina.
1992,
Pubmed
Meier,
Type B lamins remain associated with the integral nuclear envelope protein p58 during mitosis: implications for nuclear reassembly.
1994,
Pubmed
Nigg,
Targeting lamin proteins to the nuclear envelope: the role of CaaX box modifications.
1992,
Pubmed
Ohguro,
Carboxyl methylation and farnesylation of transducin gamma-subunit synergistically enhance its coupling with metarhodopsin II.
1991,
Pubmed
Padan,
Isolation and characterization of the Drosophila nuclear envelope otefin cDNA.
1990,
Pubmed
Paine,
The oocyte nucleus isolated in oil retains in vivo structure and functions.
1992,
Pubmed
,
Xenbase
Reiss,
Sequence requirement for peptide recognition by rat brain p21ras protein farnesyltransferase.
1991,
Pubmed
Schafer,
Protein prenylation: genes, enzymes, targets, and functions.
1992,
Pubmed
Schmidt-Zachmann,
Nuclear export of proteins: the role of nuclear retention.
1993,
Pubmed
,
Xenbase
Senior,
Integral membrane proteins specific to the inner nuclear membrane and associated with the nuclear lamina.
1988,
Pubmed
Simos,
The inner nuclear membrane protein p58 associates in vivo with a p58 kinase and the nuclear lamins.
1992,
Pubmed
Sinensky,
The processing pathway of prelamin A.
1994,
Pubmed
Stick,
Immunological localization of the major architectural protein associated with the nuclear envelope of the Xenopus laevis oocyte.
1982,
Pubmed
,
Xenbase
Stick,
cDNA cloning of the developmentally regulated lamin LIII of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Vorburger,
Modification of nuclear lamin proteins by a mevalonic acid derivative occurs in reticulocyte lysates and requires the cysteine residue of the C-terminal CXXM motif.
1989,
Pubmed
Worman,
The lamin B receptor of the nuclear envelope inner membrane: a polytopic protein with eight potential transmembrane domains.
1990,
Pubmed
Worman,
A lamin B receptor in the nuclear envelope.
1988,
Pubmed
