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Expression of the human sodium/proton exchanger NHE-1 in Xenopus laevis oocytes enhances sodium/proton exchange activity and establishes sodium/lithium countertransport.
Busch S
,
Burckhardt BC
,
Siffert W
.
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We investigated whether the human sodium/proton (Na+/H+) exchanger isoform 1 (NHE-1) can mediate sodium/lithium (Na+/Li+) countertransport. Using the Xenopus laevis oocyte expression system we determined amiloride-sensitive Li+ uptake, a measure of Na+/H+ exchange, in oocytes injected with water or NHE-1 cRNA. Amiloride-sensitive Li+ uptake was three- to tenfold enhanced over control in NHE-1 cRNA-injected cells and was selectively inhibited by 0.01 microM HOE 694 [i.e. (3-methylsulphonyl-4-piperidinobenzoyl) guanidine methanesulphonate]. The endogenously present Na+/H+ exchanger was insensitive to HOE 694. After acidification of oocytes from pH 7.7 to 6.8, amiloride-sensitive Li+ uptake was four- to tenfold higher in NHE-1 cRNA-injected cells than in controls. Li+ efflux from control oocytes was independent of extracellular Na+, indicating that these cells expressed no measurable Na+/Li+ countertransport activity. In NHE-1 cRNA-injected oocytes, Li+ efflux was distinctly enhanced by extracellular Na+ ions. This Na(+)-dependent Li+ efflux was inhibited by ethylisopropylamiloride, phloretin and by cytosolic acidification. The data show that expression of the NHE-1 in X. laevis oocytes induces the expression of Na+/Li+ countertransport. The data confirm that Na+/H+ exchange and Na+/Li+ countertransport are mediated by the same transport system.
Aronson,
Kinetic properties of the plasma membrane Na+-H+ exchanger.
1985, Pubmed
Aronson,
Kinetic properties of the plasma membrane Na+-H+ exchanger.
1985,
Pubmed
Borgese,
Cloning and expression of a cAMP-activated Na+/H+ exchanger: evidence that the cytoplasmic domain mediates hormonal regulation.
1992,
Pubmed
Burckhardt,
Pathways of NH3/NH4+ permeation across Xenopus laevis oocyte cell membrane.
1992,
Pubmed
,
Xenbase
Burckhardt,
Proton transport mechanism in the cell membrane of Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Canessa,
Kinetic abnormalities of the red blood cell sodium-proton exchange in hypertensive patients.
1991,
Pubmed
Canessa,
Kinetic properties of Na+/H+ exchange and Li+/Na+, Na+/Na+, and Na+/Li+ exchanges of human red cells.
1989,
Pubmed
Canessa,
Genetic differences in lithium-sodium exchange and regulation of the sodium-hydrogen exchanger in essential hypertension.
1988,
Pubmed
Cicirelli,
Internal pH of Xenopus oocytes: a study of the mechanism and role of pH changes during meiotic maturation.
1983,
Pubmed
,
Xenbase
Corry,
Increased Na/H antiport activity and abundance in uremic red blood cells.
1993,
Pubmed
Counillon,
Pharmacological characterization of stably transfected Na+/H+ antiporter isoforms using amiloride analogs and a new inhibitor exhibiting anti-ischemic properties.
1993,
Pubmed
Duhm,
Studies on lithium transport across the red cell membrane. V. On the nature of the Na+-dependent Li+ countertransport system of mammalian erythrocytes.
1979,
Pubmed
Escobales,
Na+/Na+ exchange and Na+/H+ antiport in rabbit erythrocytes: two distinct transport systems.
1991,
Pubmed
Escobales,
Amiloride-sensitive Na+ transport in human red cells: evidence for a Na/H exchange system.
1986,
Pubmed
Fliegel,
The Na+/H+ exchanger: an update on structure, regulation and cardiac physiology.
1993,
Pubmed
Franchi,
Functional expression of a human Na+/H+ antiporter gene transfected into antiporter-deficient mouse L cells.
1986,
Pubmed
Goldin,
Maintenance of Xenopus laevis and oocyte injection.
1992,
Pubmed
,
Xenbase
Guizouarn,
Role of protein phosphorylation and dephosphorylation in activation and desensitization of the cAMP-dependent Na+/H+ antiport.
1993,
Pubmed
Huot,
Na(+)-H+ exchanger and its role in essential hypertension and diabetes mellitus.
1991,
Pubmed
Kahn,
Sodium-lithium exchange and sodium-proton exchange are mediated by the same transport system in sarcolemmal vesicles from bovine superior mesenteric artery.
1989,
Pubmed
Krolewski,
Predisposition to hypertension and susceptibility to renal disease in insulin-dependent diabetes mellitus.
1988,
Pubmed
Lee,
pH changes associated with meiotic maturation in oocytes of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Levine,
Kinetics and regulation of three cloned mammalian Na+/H+ exchangers stably expressed in a fibroblast cell line.
1993,
Pubmed
Mangili,
Increased sodium-lithium countertransport activity in red cells of patients with insulin-dependent diabetes and nephropathy.
1988,
Pubmed
Merritt,
SK&F 96365, a novel inhibitor of receptor-mediated calcium entry.
1990,
Pubmed
Morgan,
Interactions of external and internal H+ and Na+ with Na+/Na+ and Na+/H+ exchange of rabbit red cells: evidence for a common pathway.
1990,
Pubmed
Orlowski,
Heterologous expression and functional properties of amiloride high affinity (NHE-1) and low affinity (NHE-3) isoforms of the rat Na/H exchanger.
1993,
Pubmed
Pouysségur,
A specific mutation abolishing Na+/H+ antiport activity in hamster fibroblasts precludes growth at neutral and acidic pH.
1984,
Pubmed
Rosskopf,
Membrane sodium-proton exchange and primary hypertension.
1993,
Pubmed
Rosskopf,
Hypertensive sodium-proton exchanger phenotype persists in immortalized lymphoblasts from essential hypertensive patients. A cell culture model for human hypertension.
1993,
Pubmed
Sardet,
Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter.
1989,
Pubmed
Sasaki,
Regulation mechanisms of intracellular pH of Xenopus laevis oocyte.
1992,
Pubmed
,
Xenbase
Towle,
Characterization of an endogenous Na+/H+ antiporter in Xenopus laevis oocytes.
1991,
Pubmed
,
Xenbase
Tse,
Structure/function studies of the epithelial isoforms of the mammalian Na+/H+ exchanger gene family.
1993,
Pubmed
