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EMBO J
1994 Dec 15;1324:5910-21. doi: 10.1002/j.1460-2075.1994.tb06936.x.
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Isolation and characterization of krp, a dibasic endopeptidase required for cell viability in the fission yeast Schizosaccharomyces pombe.
Davey J
,
Davis K
,
Imai Y
,
Yamamoto M
,
Matthews G
.
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The activation of pro-hormones and many precursor proteins involves cleavage by endopeptidases belonging to the subtilisin-like family of enzymes. Here we describe the isolation and characterization of the first member of this family from the fission yeast Schizosaccharomyces pombe. The enzyme, which has been named krp for KEX2-related protease, is a type I membrane-bound endopeptidase that cleaves substrates after pairs of dibasic residues. It appears to be synthesized as a pre-pro-protein that is likely to undergo processing following translocation into the endoplasmic reticulum. Processing has been characterized in a cell-free translation/translocation system prepared from Xenopus eggs. Krp is N-glycosylated on all five of its potential sites and both the pre-sequence and the pro-sequence are quickly removed following translocation, the latter probably by autocatalytic cleavage. The inhibitor profile of krp broadly reflects the known properties of the eukaryotic subtilisin proteases, while its pH and Ca2+ dependence are consistent with it being active within the secretory pathway. One of its physiological substrates is likely to be the pheromone precursor pro-P-factor, which it is shown to process in an in vitro system, but identification of other substrates is complicated because, unlike other members of this family, krp is essential for cell viability.
Azaryan,
Purification and characterization of a paired basic residue-specific yeast aspartic protease encoded by the YAP3 gene. Similarity to the mammalian pro-opiomelanocortin-converting enzyme.
1993, Pubmed
Azaryan,
Purification and characterization of a paired basic residue-specific yeast aspartic protease encoded by the YAP3 gene. Similarity to the mammalian pro-opiomelanocortin-converting enzyme.
1993,
Pubmed
Basco,
Processing of yeast exoglucanase (beta-glucosidase) in a KEX2-dependent manner.
1990,
Pubmed
Basi,
TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility.
1993,
Pubmed
Bos,
TGN38 is maintained in the trans-Golgi network by a tyrosine-containing motif in the cytoplasmic domain.
1993,
Pubmed
Bourbonnais,
Heterologous expression of peptide hormone precursors in the yeast Saccharomyces cerevisiae. Evidence for a novel prohormone endoprotease with specificity for monobasic amino acids.
1991,
Pubmed
Bourbonnais,
Isolation and characterization of S. cerevisiae mutants defective in somatostatin expression: cloning and functional role of a yeast gene encoding an aspartyl protease in precursor processing at monobasic cleavage sites.
1993,
Pubmed
Brenner,
Structural and enzymatic characterization of a purified prohormone-processing enzyme: secreted, soluble Kex2 protease.
1992,
Pubmed
Bretscher,
Cholesterol and the Golgi apparatus.
1993,
Pubmed
Burke,
The transmembrane and flanking sequences of beta 1,2-N-acetylglucosaminyltransferase I specify medial-Golgi localization.
1992,
Pubmed
Chapman,
Retrieval of TGN proteins from the cell surface requires endosomal acidification.
1994,
Pubmed
Davey,
Mating pheromones of the fission yeast Schizosaccharomyces pombe: purification and structural characterization of M-factor and isolation and analysis of two genes encoding the pheromone.
1992,
Pubmed
Dmochowska,
Yeast KEX1 gene encodes a putative protease with a carboxypeptidase B-like function involved in killer toxin and alpha-factor precursor processing.
1987,
Pubmed
Egel-Mitani,
A novel aspartyl protease allowing KEX2-independent MF alpha propheromone processing in yeast.
1990,
Pubmed
Errede,
A conserved kinase cascade for MAP kinase activation in yeast.
1993,
Pubmed
Fabre,
Role of the proregion in the production and secretion of the Yarrowia lipolytica alkaline extracellular protease.
1991,
Pubmed
Fukui,
Role of a ras homolog in the life cycle of Schizosaccharomyces pombe.
1986,
Pubmed
Fuller,
Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease.
1989,
Pubmed
Fuller,
Intracellular targeting and structural conservation of a prohormone-processing endoprotease.
1989,
Pubmed
Germain,
Processing of Kex2 pro-region at two interchangeable cleavage sites.
1993,
Pubmed
Germain,
Expression of the Saccharomyces cerevisiae Kex2p endoprotease in inset cells. Evidence for a carboxy-terminal autoprocessing event.
1992,
Pubmed
Germain,
The pro-region of the Kex2 endoprotease of Saccharomyces cerevisiae is removed by self-processing.
1992,
Pubmed
Humphrey,
Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence.
1993,
Pubmed
Ikemura,
Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli.
1987,
Pubmed
Imai,
The fission yeast mating pheromone P-factor: its molecular structure, gene structure, and ability to induce gene expression and G1 arrest in the mating partner.
1994,
Pubmed
Jackson,
Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.
1990,
Pubmed
Julius,
Yeast alpha factor is processed from a larger precursor polypeptide: the essential role of a membrane-bound dipeptidyl aminopeptidase.
1983,
Pubmed
Julius,
Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-alpha-factor.
1984,
Pubmed
Julius,
Glycosylation and processing of prepro-alpha-factor through the yeast secretory pathway.
1984,
Pubmed
Kitamura,
The Schizosaccharomyces pombe mam2 gene encodes a putative pheromone receptor which has a significant homology with the Saccharomyces cerevisiae Ste2 protein.
1991,
Pubmed
Kjaerulff,
Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3.
1994,
Pubmed
Kocher,
Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.
1989,
Pubmed
Kozak,
Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo.
,
Pubmed
Kozak,
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
1986,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Lau,
Substrate recognition by oligosaccharyl transferase. Inhibition of co-translational glycosylation by acceptor peptides.
1983,
Pubmed
Lazarovits,
A single amino acid change in the cytoplasmic domain allows the influenza virus hemagglutinin to be endocytosed through coated pits.
1988,
Pubmed
Luzio,
Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).
1990,
Pubmed
Machamer,
Targeting and retention of Golgi membrane proteins.
1993,
Pubmed
Matthews,
Autocatalytic maturation of the prohormone convertase PC2.
1994,
Pubmed
,
Xenbase
Matthews,
A highly efficient, cell-free translation/translocation system prepared from Xenopus eggs.
1991,
Pubmed
,
Xenbase
Maundrell,
nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.
1990,
Pubmed
Michaelis,
STE6, the yeast a-factor transporter.
1993,
Pubmed
Mizuno,
Yeast KEX2 genes encodes an endopeptidase homologous to subtilisin-like serine proteases.
1988,
Pubmed
Moehring,
Expression of mouse furin in a Chinese hamster cell resistant to Pseudomonas exotoxin A and viruses complements the genetic lesion.
1993,
Pubmed
Molloy,
Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen.
1992,
Pubmed
Molloy,
Intracellular trafficking and activation of the furin proprotein convertase: localization to the TGN and recycling from the cell surface.
1994,
Pubmed
Moreno,
Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
1991,
Pubmed
Munro,
Sequences within and adjacent to the transmembrane segment of alpha-2,6-sialyltransferase specify Golgi retention.
1991,
Pubmed
Nakayama,
Consensus sequence for precursor processing at mono-arginyl sites. Evidence for the involvement of a Kex2-like endoprotease in precursor cleavages at both dibasic and mono-arginyl sites.
1992,
Pubmed
Nakayama,
Cloning and functional expression of a novel endoprotease involved in prohormone processing at dibasic sites.
1991,
Pubmed
Nebes,
Activation of the proteinase B precursor of the yeast Saccharomyces cerevisiae by autocatalysis and by an internal sequence.
1991,
Pubmed
Nielsen,
The ras1 function of Schizosaccharomyces pombe mediates pheromone-induced transcription.
1992,
Pubmed
Nielsen,
Signal transduction during mating and meiosis in S. pombe.
1993,
Pubmed
Nilsson,
The membrane spanning domain of beta-1,4-galactosyltransferase specifies trans Golgi localization.
1991,
Pubmed
Nilsson,
Kin recognition. A model for the retention of Golgi enzymes.
1993,
Pubmed
Obara,
Isolation and characterization of a gene encoding a G-protein alpha subunit from Schizosaccharomyces pombe: involvement in mating and sporulation pathways.
1991,
Pubmed
Pearse,
Clathrin, adaptors, and sorting.
1990,
Pubmed
Pelham,
Recycling of proteins between the endoplasmic reticulum and Golgi complex.
1991,
Pubmed
Pfeffer,
Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi.
1987,
Pubmed
Rehemtulla,
Regulation of PACE propeptide-processing activity: requirement for a post-endoplasmic reticulum compartment and autoproteolytic activation.
1992,
Pubmed
Rogers,
Saccharomyces cerevisiae killer expression mutant kex2 has altered secretory proteins and glycoproteins.
1979,
Pubmed
Rothstein,
One-step gene disruption in yeast.
1983,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Shennan,
Characterization of PC2, a mammalian Kex2 homologue, following expression of the cDNA in microinjected Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Shennan,
Site-directed mutagenesis and expression of PC2 in microinjected Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Silen,
Analysis of prepro-alpha-lytic protease expression in Escherichia coli reveals that the pro region is required for activity.
1989,
Pubmed
Smeekens,
Identification of a human insulinoma cDNA encoding a novel mammalian protein structurally related to the yeast dibasic processing protease Kex2.
1990,
Pubmed
Sugimoto,
Schizosaccharomyces pombe ste11+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual development.
1991,
Pubmed
Takahashi,
A mutation of furin causes the lack of precursor-processing activity in human colon carcinoma LoVo cells.
1993,
Pubmed
Tanaka,
Schizosaccharomyces pombe map3+ encodes the putative M-factor receptor.
1993,
Pubmed
Tang,
The transmembrane domain of N-glucosaminyltransferase I contains a Golgi retention signal.
1992,
Pubmed
Trowbridge,
Signal-dependent membrane protein trafficking in the endocytic pathway.
1993,
Pubmed
Wang,
Chemical synthesis of the M-factor mating pheromone from Schizosaccharomyces pombe.
1994,
Pubmed
Wilcox,
Mutation of a tyrosine localization signal in the cytosolic tail of yeast Kex2 protease disrupts Golgi retention and results in default transport to the vacuole.
1992,
Pubmed
Wilcox,
Posttranslational processing of the prohormone-cleaving Kex2 protease in the Saccharomyces cerevisiae secretory pathway.
1991,
Pubmed
Wong,
The 17-residue transmembrane domain of beta-galactoside alpha 2,6-sialyltransferase is sufficient for Golgi retention.
1992,
Pubmed
Wong,
The SXYQRL sequence in the cytoplasmic domain of TGN38 plays a major role in trans-Golgi network localization.
1993,
Pubmed
