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Single channel properties of cloned NMDA receptors in a human cell line: comparison with results from Xenopus oocytes.
Stern P
,
Cik M
,
Colquhoun D
,
Stephenson FA
.
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1. Human embryonic kidney (HEK) 293 cells were transiently transfected with cDNAs encoding the NR1a-NMDA epsilon 1[NR2A] subunit combination of the NMDA receptor. Single channel behaviour was recorded from outside-out membrane patches, with the aim of comparing the results with those, recorded under the same conditions, from Xenopus oocytes injected with messenger RNA coding for the NR1a-NR2A combination. 2. Single channels in HEK 293 cells showed a main conductance level of 51.4 +/- 2.4 pS, compared with 50.1 +/- 1.4 pS for channels in oocytes. A subconductance level of 38.1 +/- 2.1 pS was found in HEK 293 cells, compared with 38.3 +/- 1.3 pS in oocytes. The frequencies of transitions between the shut and the two conductance levels were also very similar. 3. Distributions of shut times could be fitted with five exponential components. In HEK 293 cells the first three of these components had time constants of 39 +/- 4 microseconds, 0.54 +/- 0.04 ms and 9.94 +/- 1.3 ms; in oocytes the values were 69 +/- 35 microseconds, 0.54 +/- 0.15 ms and 6.53 +/- 4.6 ms, respectively. The relative areas of the components were also similar in the two systems. 4. The distribution of all apparent open times for the sublevels was fitted with two exponential components giving time constants of 0.18 +/- 0.02 ms and 1.31 +/- 0.17 ms (for HEK cells) or of 0.31 +/- 0.36 ms and 1.31 +/- 1.1 ms (for oocytes).(ABSTRACT TRUNCATED AT 250 WORDS)
Blatz,
Correcting single channel data for missed events.
1986, Pubmed
Blatz,
Correcting single channel data for missed events.
1986,
Pubmed
Buller,
Altered patterns of N-linked glycosylation of the Torpedo acetylcholine receptor expressed in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Buller,
Functional acetylcholine receptors expressed in Xenopus oocytes after injection of Torpedo beta, gamma, and delta subunit RNAs are a consequence of endogenous oocyte gene expression.
1990,
Pubmed
,
Xenbase
Chazot,
Immunological detection of the NMDAR1 glutamate receptor subunit expressed in embryonic kidney 293 cells and in rat brain.
1992,
Pubmed
Cik,
Optimal expression of cloned NMDAR1/NMDAR2A heteromeric glutamate receptors: a biochemical characterization.
1993,
Pubmed
Cull-Candy,
Multiple-conductance channels activated by excitatory amino acids in cerebellar neurons.
,
Pubmed
Egebjerg,
Ca2+ permeability of unedited and edited versions of the kainate selective glutamate receptor GluR6.
1993,
Pubmed
,
Xenbase
Gibb,
Expression of cloned receptor subunits produces multiple receptors.
1990,
Pubmed
,
Xenbase
Gibb,
Activation of N-methyl-D-aspartate receptors by L-glutamate in cells dissociated from adult rat hippocampus.
1992,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Howe,
Currents through single glutamate receptor channels in outside-out patches from rat cerebellar granule cells.
1991,
Pubmed
Meguro,
Functional characterization of a heteromeric NMDA receptor channel expressed from cloned cDNAs.
1992,
Pubmed
,
Xenbase
Mishina,
Molecular distinction between fetal and adult forms of muscle acetylcholine receptor.
,
Pubmed
,
Xenbase
Papke,
The role of the beta 4-subunit in determining the kinetic properties of rat neuronal nicotinic acetylcholine alpha 3-receptors.
1991,
Pubmed
,
Xenbase
Sigworth,
Data transformations for improved display and fitting of single-channel dwell time histograms.
1987,
Pubmed
Stern,
Single-channel conductances of NMDA receptors expressed from cloned cDNAs: comparison with native receptors.
1992,
Pubmed
,
Xenbase
