Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Transcription factors required for the expression of Xenopus laevis selenocysteine tRNA in vitro.
Meissner W
,
Wanandi I
,
Carbon P
,
Krol A
,
Seifart KH
.
???displayArticle.abstract???
It has previously been reported that transcription in vivo of the tRNA(Sec) gene requires three promoter elements, a PSE and a TATA-box upstream of the coding region which are functionally interchangeable with the U6 snRNA gene counterparts and an internal B-block, resembling that of classical tRNA genes (1). We have established an in vitro transcription system from HeLa cells in which three factors, which are either essential for or stimulate transcription were identified. Apart from the TATA-binding protein TBP, the PSE-binding protein PBP was found to be essentially required for expression of the gene. Depletion of PBP from cell extracts by PSE-oligonucleotides abolished tRNA(Sec) transcription, which could be reconstituted by readdition of partially purified PBP. Addition of increasing amounts of recombinant human TBP to an S100 extract stimulated transcription of the tRNA(Sec), the mouse U6 snRNA and the human Y3 genes, an effect which was not observed in the case of a TATA-less tRNA gene. Purified human TFIIA strongly stimulated tRNA(Sec) transcription in a fashion depending on the concentration of TBP. Surprisingly, partially purified TFIIIC was shown to be dispensable for transcription in vitro and unable to bind the B-block of this gene in vitro, although its sequence matches the consensus for this element. Collectively, these data suggest that the mechanism by which transcription complexes are formed on the tRNA(Sec) gene is dramatically different from that observed for classical tRNA genes and much more resembles that observed for externally controlled pol III genes.
Bernués,
Common and unique transcription factor requirements of human U1 and U6 snRNA genes.
1993, Pubmed
Bernués,
Common and unique transcription factor requirements of human U1 and U6 snRNA genes.
1993,
Pubmed
Brow,
Transcription of a yeast U6 snRNA gene requires a polymerase III promoter element in a novel position.
1990,
Pubmed
Bungert,
Transcription factor IIA is inactivated during terminal differentiation of avian erythroid cells.
1992,
Pubmed
Buratowski,
Five intermediate complexes in transcription initiation by RNA polymerase II.
1989,
Pubmed
Burnol,
Basal promoter and enhancer element of yeast U6 snRNA gene.
1993,
Pubmed
Burnol,
TFIIIC relieves repression of U6 snRNA transcription by chromatin.
1993,
Pubmed
,
Xenbase
Carbon,
A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.
1987,
Pubmed
,
Xenbase
Carbon,
Transcription of the Xenopus laevis selenocysteine tRNA(Ser)Sec gene: a system that combines an internal B box and upstream elements also found in U6 snRNA genes.
1991,
Pubmed
,
Xenbase
Chiang,
Unique TATA-binding protein-containing complexes and cofactors involved in transcription by RNA polymerases II and III.
1993,
Pubmed
Eschenlauer,
Architecture of a yeast U6 RNA gene promoter.
1993,
Pubmed
Gabrielsen,
RNA polymerase III (C) and its transcription factors.
1991,
Pubmed
,
Xenbase
Hernandez,
TBP, a universal eukaryotic transcription factor?
1993,
Pubmed
Huet,
The TATA-binding protein participates in TFIIIB assembly on tRNA genes.
1992,
Pubmed
Jahn,
Transcription complexes for various class III genes differ in parameters of formation and stability towards salt.
1987,
Pubmed
Kassavetis,
The role of the TATA-binding protein in the assembly and function of the multisubunit yeast RNA polymerase III transcription factor, TFIIIB.
1992,
Pubmed
Kassavetis,
S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factors.
1990,
Pubmed
Kelekar,
Downregulation of RNA polymerase III transcription of the hY3 gene in vitro.
1990,
Pubmed
Kunkel,
RNA polymerase III transcription of genes that lack internal control regions.
1991,
Pubmed
,
Xenbase
Lee,
Transcription of Xenopus selenocysteine tRNA Ser (formerly designated opal suppressor phosphoserine tRNA) gene is directed by multiple 5'-extragenic regulatory elements.
1989,
Pubmed
,
Xenbase
Lee,
TFIIA induces conformational changes in TFIID via interactions with the basic repeat.
1992,
Pubmed
Lobo,
A TBP complex essential for transcription from TATA-less but not TATA-containing RNA polymerase III promoters is part of the TFIIIB fraction.
1992,
Pubmed
Lobo,
The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro.
1991,
Pubmed
Lobo,
A 7 bp mutation converts a human RNA polymerase II snRNA promoter into an RNA polymerase III promoter.
1989,
Pubmed
Maldonado,
Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.
1990,
Pubmed
Margottin,
Participation of the TATA factor in transcription of the yeast U6 gene by RNA polymerase C.
1991,
Pubmed
Mattaj,
Changing the RNA polymerase specificity of U snRNA gene promoters.
1988,
Pubmed
,
Xenbase
Meissner,
Transcription factor IIA stimulates the expression of classical polIII-genes.
1993,
Pubmed
Moenne,
The U6 gene of Saccharomyces cerevisiae is transcribed by RNA polymerase C (III) in vivo and in vitro.
1990,
Pubmed
Murphy,
Oct-1 and Oct-2 potentiate functional interactions of a transcription factor with the proximal sequence element of small nuclear RNA genes.
1992,
Pubmed
Myslinski,
Promoter strength and structure dictate module composition in RNA polymerase III transcriptional activator elements.
1993,
Pubmed
,
Xenbase
Myslinski,
Point mutations 5' to the tRNA selenocysteine TATA box alter RNA polymerase III transcription by affecting the binding of TBP.
1993,
Pubmed
,
Xenbase
Nakajima,
Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID.
1988,
Pubmed
Reinberg,
Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site.
1987,
Pubmed
Rigby,
Three in one and one in three: it all depends on TBP.
1993,
Pubmed
Sadowski,
Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE.
1993,
Pubmed
Schneider,
Purification of human transcription factor IIIC and its binding to the gene for ribosomal 5S RNA.
1989,
Pubmed
,
Xenbase
Shapiro,
A high-efficiency HeLa cell nuclear transcription extract.
1988,
Pubmed
,
Xenbase
Simmen,
Cofractionation of the TATA-binding protein with the RNA polymerase III transcription factor TFIIIB.
1992,
Pubmed
Taggart,
The TATA-binding protein and associated factors are components of pol III transcription factor TFIIIB.
1992,
Pubmed
Timmers,
The mammalian TFIID protein is present in two functionally distinct complexes.
1991,
Pubmed
Timmers,
Composition of transcription factor B-TFIID.
1992,
Pubmed
Usuda,
Affinity purification of transcription factor IIA from HeLa cell nuclear extracts.
1991,
Pubmed
Van Dyke,
Two forms of transcription factor TFIIIC in extracts from HeLa cells.
1987,
Pubmed
Waldschmidt,
TFIIA is required for in vitro transcription of mammalian U6 genes by RNA polymerase III.
1992,
Pubmed
Waldschmidt,
Identification of transcription factors required for the expression of mammalian U6 genes in vitro.
1991,
Pubmed
Waldschmidt,
Purification of transcription factor IIIB from HeLa cells.
1988,
Pubmed
,
Xenbase
Wanandi,
Mammalian transcription factor PBP. Characterization of its binding properties to the proximal sequence element of U6 genes.
1993,
Pubmed
Weingart,
Transcription of the alpha A-globin gene of the duck. Development of a homologous in vitro system and identification of trans-acting factors.
1989,
Pubmed
White,
Mechanism of TATA-binding protein recruitment to a TATA-less class III promoter.
1992,
Pubmed
Wolin,
Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome.
1983,
Pubmed
