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EMBO J
1993 Aug 01;128:3123-32. doi: 10.1002/j.1460-2075.1993.tb05981.x.
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The cdc2-related protein p40MO15 is the catalytic subunit of a protein kinase that can activate p33cdk2 and p34cdc2.
Poon RY
,
Yamashita K
,
Adamczewski JP
,
Hunt T
,
Shuttleworth J
.
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Activation of the cyclin-dependent protein kinases p34cdc2 and p33cdk2 requires binding with a cyclin partner and phosphorylation on the first threonine residue in the sequence THEVVTLWYRAPE. We present evidence that this threonine residue, number 160 in p33cdk2, can be specifically phosphorylated by a cdc2-related protein kinase from Xenopus oocytes called p40MO15. Binding to cyclin A and phosphorylation of this threonine are both required to activate fully the histone H1 kinase activity of p33cdk2. In cell extracts, a portion of p40MO15 is found in a high molecular weight complex that is considerably more active than a lower molecular weight form. Wild-type MO15 protein expressed in bacteria does not possess kinase activity, but acquires p33cdk2-T160 kinase activity after incubation with cell extract and ATP. We conclude that p40MO15 corresponds to CAK (cdc2/cdk2 activating kinase) and speculate that, like p33cdk2 and p34cdc2, p40MO15 requires activation by phosphorylation and association with a companion subunit.
Bandara,
Cyclin A and the retinoblastoma gene product complex with a common transcription factor.
1991, Pubmed
Bandara,
Cyclin A and the retinoblastoma gene product complex with a common transcription factor.
1991,
Pubmed
Booher,
Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccharomyces pombe.
1986,
Pubmed
Brizuela,
Activation of human CDC2 protein as a histone H1 kinase is associated with complex formation with the p62 subunit.
1989,
Pubmed
Connell-Crowley,
Phosphorylation independent activation of human cyclin-dependent kinase 2 by cyclin A in vitro.
1993,
Pubmed
,
Xenbase
Desai,
Activation of human cyclin-dependent kinases in vitro.
1992,
Pubmed
Ducommun,
cdc2 phosphorylation is required for its interaction with cyclin.
1991,
Pubmed
,
Xenbase
Dulić,
Association of human cyclin E with a periodic G1-S phase protein kinase.
1992,
Pubmed
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Dunphy,
The cdc25 protein contains an intrinsic phosphatase activity.
1991,
Pubmed
Elledge,
A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1.
1991,
Pubmed
,
Xenbase
Erikson,
Biochemical characterization of the p34cdc2 protein kinase component of purified maturation-promoting factor from Xenopus eggs.
1989,
Pubmed
,
Xenbase
Ershler,
Novel CDC2-related protein kinases produced in murine hematopoietic stem cells.
1993,
Pubmed
Featherstone,
Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase.
1991,
Pubmed
Fesquet,
The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues.
1993,
Pubmed
,
Xenbase
Fleig,
Regulation of cdc2 activity in Schizosaccharomyces pombe: the role of phosphorylation.
1991,
Pubmed
Fleig,
A dominant negative allele of p34cdc2 shows altered phosphoamino acid content and sequesters p56cdc13 cyclin.
1992,
Pubmed
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
,
Xenbase
Gautier,
cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
1991,
Pubmed
,
Xenbase
Gould,
Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function.
1991,
Pubmed
Gu,
Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15.
1992,
Pubmed
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Hata,
cDNA cloning of a novel cdc2+/CDC28-related protein kinase from rice.
1991,
Pubmed
Hirai,
Isolation and characterization of goldfish cdk2, a cognate variant of the cell cycle regulator cdc2.
1992,
Pubmed
,
Xenbase
Hoffmann,
Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.
1991,
Pubmed
Kamps,
Determination of phosphoamino acid composition by acid hydrolysis of protein blotted to Immobilon.
1991,
Pubmed
Kobayashi,
Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.
1992,
Pubmed
,
Xenbase
Kobayashi,
Cyclins and their partners during Xenopus oocyte maturation.
1991,
Pubmed
,
Xenbase
Koff,
Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle.
1992,
Pubmed
Koff,
Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family.
1991,
Pubmed
,
Xenbase
Krek,
Cell cycle regulation of vertebrate p34cdc2 activity: identification of Thr161 as an essential in vivo phosphorylation site.
1992,
Pubmed
Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed
Lee,
cdc25+ encodes a protein phosphatase that dephosphorylates p34cdc2.
1992,
Pubmed
,
Xenbase
Lee,
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
,
Pubmed
Lees,
Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner.
1992,
Pubmed
Lew,
Brain proline-directed protein kinase is a neurofilament kinase which displays high sequence homology to p34cdc2.
1992,
Pubmed
Lorca,
Dephosphorylation of cdc2 on threonine 161 is required for cdc2 kinase inactivation and normal anaphase.
1992,
Pubmed
,
Xenbase
Meyerson,
A family of human cdc2-related protein kinases.
1992,
Pubmed
Milarski,
Cloning and characterization of Xenopus cdc2, a component of MPF.
1991,
Pubmed
,
Xenbase
Millar,
p80cdc25 mitotic inducer is the tyrosine phosphatase that activates p34cdc2 kinase in fission yeast.
1991,
Pubmed
Nasmyth,
Control of the yeast cell cycle by the Cdc28 protein kinase.
1993,
Pubmed
Ninomiya-Tsuji,
Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation.
1991,
Pubmed
,
Xenbase
Norbury,
Animal cell cycles and their control.
1992,
Pubmed
Norbury,
Regulatory phosphorylation of the p34cdc2 protein kinase in vertebrates.
1991,
Pubmed
,
Xenbase
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Paris,
Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2.
1991,
Pubmed
,
Xenbase
Parker,
p107wee1 is a dual-specificity kinase that phosphorylates p34cdc2 on tyrosine 15.
1992,
Pubmed
Pines,
Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
1989,
Pubmed
Rosenblatt,
Human cyclin-dependent kinase 2 is activated during the S and G2 phases of the cell cycle and associates with cyclin A.
1992,
Pubmed
Russell,
Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog.
1987,
Pubmed
Shamu,
Sister chromatid separation in frog egg extracts requires DNA topoisomerase II activity during anaphase.
1992,
Pubmed
,
Xenbase
Shuttleworth,
p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Simon,
KIN28, a yeast split gene coding for a putative protein kinase homologous to CDC28.
1986,
Pubmed
Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase
Solomon,
Role of phosphorylation in p34cdc2 activation: identification of an activating kinase.
1992,
Pubmed
,
Xenbase
Tsai,
Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase.
1991,
Pubmed
,
Xenbase
Whitfield,
The A- and B-type cyclins of Drosophila are accumulated and destroyed in temporally distinct events that define separable phases of the G2-M transition.
1990,
Pubmed
Xiong,
Population explosion in the cyclin family.
1991,
Pubmed
