Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Diverse peroxisome proliferator-activated receptors bind to the peroxisome proliferator-responsive elements of the rat hydratase/dehydrogenase and fatty acyl-CoA oxidase genes but differentially induce expression.
Marcus SL
,
Miyata KS
,
Zhang B
,
Subramani S
,
Rachubinski RA
,
Capone JP
.
???displayArticle.abstract???
The ability of peroxisome proliferator-activated receptors (PPARs) to induce expression of a reporter gene linked to a peroxisome proliferator-responsive element (PPRE) from either the rat enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase gene or acyl-CoA oxidase [acyl-CoA:oxygen 2-oxidoreductase, EC 1.3.3.6] gene was examined by transient transfection assays in COS cells. Mouse and rat PPARs, as well as Xenopus PPAR alpha (xPPAR alpha) could induce expression of a reporter gene linked to the hydratase/dehydrogenase PPRE in the presence of the peroxisome proliferators ciprofibrate or Wy-14,643, whereas xPPAR beta and xPPAR gamma were ineffective. A similar induction of expression of a reporter gene linked to the acyl-CoA oxidase PPRE was observed with all PPARs except xPPAR beta. Extracts from cells transfected with PPAR-encoding genes contained factors that bound to both PPREs. In vitro synthesized PPARs could interact weakly with both PPREs; however, binding of each PPAR to both PPREs was significantly increased by the addition of COS cell nuclear extracts, demonstrating that efficient PPAR/DNA binding requires auxiliary cofactors. One cofactor was identified as the 9-cis-retinoic acid receptor, RXR alpha (retinoid X receptor alpha). Cooperative DNA binding and heteromerization between RXR alpha and each of the PPARs could be seen with both PPREs. Our results demonstrate that PPAR/PPRE binding and cooperativity with RXR alpha (and other cofactors) are obligatory but not necessarily sufficient for peroxisome proliferator-dependent transcription induction and that distinct PPREs can selectively mediate induction by particular PPARs.
Andrews,
A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells.
1991, Pubmed
Andrews,
A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells.
1991,
Pubmed
Burnside,
A nuclear factor that enhances binding of thyroid hormone receptors to thyroid hormone response elements.
1990,
Pubmed
Cattley,
Differences between the promoting activities of the peroxisome proliferator WY-14,643 and phenobarbital in rat liver.
1989,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Dreyer,
Control of the peroxisomal beta-oxidation pathway by a novel family of nuclear hormone receptors.
1992,
Pubmed
,
Xenbase
Glass,
Multiple cell type-specific proteins differentially regulate target sequence recognition by the alpha retinoic acid receptor.
1990,
Pubmed
Green,
A versatile in vivo and in vitro eukaryotic expression vector for protein engineering.
1988,
Pubmed
Göttlicher,
Fatty acids activate a chimera of the clofibric acid-activated receptor and the glucocorticoid receptor.
1992,
Pubmed
Issemann,
Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators.
1990,
Pubmed
Kliewer,
Retinoid X receptor-COUP-TF interactions modulate retinoic acid signaling.
1992,
Pubmed
Kliewer,
Convergence of 9-cis retinoic acid and peroxisome proliferator signalling pathways through heterodimer formation of their receptors.
1992,
Pubmed
Lazarow,
Biogenesis of peroxisomes.
1985,
Pubmed
Leid,
Purification, cloning, and RXR identity of the HeLa cell factor with which RAR or TR heterodimerizes to bind target sequences efficiently.
1992,
Pubmed
Mangelsdorf,
A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR.
1991,
Pubmed
Marsman,
Relationship of hepatic peroxisome proliferation and replicative DNA synthesis to the hepatocarcinogenicity of the peroxisome proliferators di(2-ethylhexyl)phthalate and [4-chloro-6-(2,3-xylidino)-2-pyrimidinylthio]acetic acid (Wy-14,643) in rats.
1988,
Pubmed
Osumi,
Two cis-acting regulatory sequences in the peroxisome proliferator-responsive enhancer region of rat acyl-CoA oxidase gene.
1991,
Pubmed
Rao,
An overview of peroxisome proliferator-induced hepatocarcinogenesis.
1991,
Pubmed
Reddy,
Transcription regulation of peroxisomal fatty acyl-CoA oxidase and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase in rat liver by peroxisome proliferators.
1986,
Pubmed
Schmidt,
Identification of a new member of the steroid hormone receptor superfamily that is activated by a peroxisome proliferator and fatty acids.
1992,
Pubmed
Sharma,
Differential induction of peroxisomal and microsomal fatty-acid-oxidising enzymes by peroxisome proliferators in rat liver and kidney. Characterisation of a renal cytochrome P-450 and implications for peroxisome proliferation.
1989,
Pubmed
Tugwood,
The mouse peroxisome proliferator activated receptor recognizes a response element in the 5' flanking sequence of the rat acyl CoA oxidase gene.
1992,
Pubmed
Zhang,
Identification of a peroxisome proliferator-responsive element upstream of the gene encoding rat peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase.
1992,
Pubmed