Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1993 Mar 01;123:1229-38. doi: 10.1002/j.1460-2075.1993.tb05764.x.
Show Gene links
Show Anatomy links
The low abundance of U7 snRNA is partly determined by its Sm binding site.
Grimm C
,
Stefanovic B
,
Schümperli D
.
???displayArticle.abstract???
In transient expression studies after DNA transfection of HeLa cells, the mouse U7 gene produces only approximately 30% of the RNA produced by a mouse U1b gene. This difference persists even when the transfected genes have all their 5' and 3' flanking sequences exchanged suggesting a post-transcriptional effect. When the special U7 Sm binding site is mutated to a consensus derived from the major snRNAs (Sm-opt), the U7 RNA level increases 4- to 5-fold, whereas no RNA is detected from a U7 gene with a non-functional Sm binding site (Sm-mut). Moreover, U1b genes with the U7 Sm binding site yield reduced RNA levels. The Sm-opt site also alters the cellular behaviour of the corresponding U7 snRNA. It accumulates to a higher level in the nucleus than wild type U7 RNA, and is better immunoprecipitable with anti-Sm antibodies. Injection experiments in Xenopus oocytes indicate that the U7 genes with either Sm-opt or Sm-mut sites produce similar amounts of RNA as wild type U7, but that they differ in opposing ways in the processing of precursors to mature size U7 snRNA and in nuclear accumulation. However, in reconstitution experiments using Xenopus oocytes, we show that U7 Sm-opt RNA, despite its efficient nuclear accumulation, is not active in 3' processing of histone pre-mRNA, whereas wild type U7 RNA is assembled into functional snRNPs, which correctly process histone pre-mRNA substrate. This suggests a functional importance of the special U7 Sm sequence.
Ach,
The highly conserved U small nuclear RNA 3'-end formation signal is quite tolerant to mutation.
1987, Pubmed,
Xenbase
Ach,
The highly conserved U small nuclear RNA 3'-end formation signal is quite tolerant to mutation.
1987,
Pubmed
,
Xenbase
Birchmeier,
3' editing of mRNAs: sequence requirements and involvement of a 60-nucleotide RNA in maturation of histone mRNA precursors.
1984,
Pubmed
,
Xenbase
Birchmeier,
Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences.
1982,
Pubmed
Bond,
Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro.
1991,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Cotten,
Specific contacts between mammalian U7 snRNA and histone precursor RNA are indispensable for the in vitro 3' RNA processing reaction.
1988,
Pubmed
De Lorenzi,
Analysis of a sea urchin gene cluster coding for the small nuclear U7 RNA, a rare RNA species implicated in the 3' editing of histone precursor mRNAs.
1986,
Pubmed
Feeney,
Cytoplasmic assembly and nuclear accumulation of mature small nuclear ribonucleoprotein particles.
1989,
Pubmed
Feeney,
Nuclear exchange of the U1 and U2 snRNP-specific proteins.
1990,
Pubmed
Fresco,
Rapid inhibition of processing and assembly of small nuclear ribonucleoproteins after infection with vesicular stomatitis virus.
1987,
Pubmed
Georgiev,
Processing and nucleo-cytoplasmic transport of histone gene transcripts.
1984,
Pubmed
,
Xenbase
Gruber,
Structure of a mouse histone-encoding gene cluster.
1990,
Pubmed
Gruber,
Isolation of an active gene and of two pseudogenes for mouse U7 small nuclear RNA.
1991,
Pubmed
,
Xenbase
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Hamm,
Loop I of U1 small nuclear RNA is the only essential RNA sequence for binding of specific U1 small nuclear ribonucleoprotein particle proteins.
1988,
Pubmed
,
Xenbase
Hamm,
The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal.
1990,
Pubmed
,
Xenbase
Hernandez,
Elements required for transcription initiation of the human U2 snRNA gene coincide with elements required for snRNA 3' end formation.
1988,
Pubmed
Howard,
Functional, developmentally expressed genes for mouse U1a and U1b snRNAs contain both conserved and non-conserved transcription signals.
1986,
Pubmed
,
Xenbase
Jones,
Unexpected flexibility in an evolutionarily conserved protein-RNA interaction: genetic analysis of the Sm binding site.
1990,
Pubmed
Kambach,
Intracellular distribution of the U1A protein depends on active transport and nuclear binding to U1 snRNA.
1992,
Pubmed
,
Xenbase
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1987,
Pubmed
Kunkel,
U6 small nuclear RNA is transcribed by RNA polymerase III.
1986,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase
Lührmann,
Structure of spliceosomal snRNPs and their role in pre-mRNA splicing.
1990,
Pubmed
Lund,
Differential control of U1 small nuclear RNA expression during mouse development.
1985,
Pubmed
Marzluff,
Isolation and characterization of two linked mouse U1b small nuclear RNA genes.
1983,
Pubmed
Mattaj,
Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
1985,
Pubmed
,
Xenbase
Mattaj,
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
1986,
Pubmed
,
Xenbase
Melin,
Biochemical demonstration of complex formation of histone pre-mRNA with U7 small nuclear ribonucleoprotein and hairpin binding factors.
1992,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Michaud,
Microinjected U snRNAs are imported to oocyte nuclei via the nuclear pore complex by three distinguishable targeting pathways.
1992,
Pubmed
,
Xenbase
Mowry,
Identification of the human U7 snRNP as one of several factors involved in the 3' end maturation of histone premessenger RNA's.
1987,
Pubmed
Neuman de Vegvar,
Nucleocytoplasmic transport and processing of small nuclear RNA precursors.
1990,
Pubmed
,
Xenbase
Nigg,
Nuclear import-export: in search of signals and mechanisms.
1991,
Pubmed
Phillips,
Analysis of a gene cluster coding for the Xenopus laevis U7 snRNA.
1992,
Pubmed
,
Xenbase
Phillips,
Nucleotide sequence of the mouse U7 snRNA gene.
1991,
Pubmed
Phillips,
A transcriptional analysis of the gene encoding mouse U7 small nuclear RNA.
1992,
Pubmed
,
Xenbase
Reddy,
Small nuclear RNAs and RNA processing.
1983,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Smith,
Two-step affinity purification of U7 small nuclear ribonucleoprotein particles using complementary biotinylated 2'-O-methyl oligoribonucleotides.
1991,
Pubmed
Soldati,
Structural and functional characterization of mouse U7 small nuclear RNA active in 3' processing of histone pre-mRNA.
1988,
Pubmed
Southgate,
In vivo and in vitro expression of U7 snRNA genes: cis- and trans-acting elements required for RNA polymerase II-directed transcription.
1989,
Pubmed
Strub,
The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing.
1984,
Pubmed
,
Xenbase
Suter-Crazzolara,
Organization and transient expression of the gene for human U11 snRNA.
1991,
Pubmed
Watkins,
The Xenopus U7 snRNA-encoding gene has an unusually compact structure.
1992,
Pubmed
,
Xenbase
Wigler,
Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.
1978,
Pubmed
Yang,
Nuclear processing of the 3'-terminal nucleotides of pre-U1 RNA in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Zieve,
Cell biology of the snRNP particles.
1990,
Pubmed
