Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1992 Dec 15;8924:11895-9.
Show Gene links
Show Anatomy links
A 69-kDa RNA-binding protein from Xenopus oocytes recognizes a common motif in two vegetally localized maternal mRNAs.
Schwartz SP
,
Aisenthal L
,
Elisha Z
,
Oberman F
,
Yisraeli JK
.
???displayArticle.abstract???
Vg1 mRNA, a maternal message encoding a member of the transforming growth factor beta superfamily, undergoes localization to the vegetal cortex of Xenopus laevis oocytes during a narrow period of oogenesis. A 340-nucleotide sequence has been identified in Vg1 RNA that directs its vegetal localization [Mowry, K. L. & Melton, D. A. (1992) Science 255, 991-994]. To understand how cis- and trans-acting factors are involved in Vg1 mRNA localization, we have looked for specific interactions in vitro between oocyte proteins and Vg1 mRNA. S100 extracts of late-stage oocytes contain a protein-binding activity that protects specific regions of labeled Vg1 mRNA from degradation by RNase T1. The use of different regions of Vg1 RNA in competition reactions reveals two binding sites, both in the first half of the 3' untranslated region of Vg1 message. UV crosslinking predominantly labels a 69-kDa protein; saturation analysis and competitor studies indicate that this protein binds with a high affinity to the down-stream site, which corresponds to the 340-nucleotide vegetal localization sequence. Binding to this region is inhibited by another vegetally localized message, transforming growth factor beta 5 but is not inhibited by an animally localized RNA, An2. These data indicate that vegetally localized mRNAs share a binding motif that helps them achieve their intracellular distribution through specific RNA-protein interactions.
Akam,
The molecular basis for metameric pattern in the Drosophila embryo.
1987, Pubmed
Akam,
The molecular basis for metameric pattern in the Drosophila embryo.
1987,
Pubmed
al-Mukhtar,
An ultrastructural study of primordial germ cells, oogonia and early oocytes in Xenopus laevis.
1971,
Pubmed
,
Xenbase
Bass,
A developmentally regulated activity that unwinds RNA duplexes.
1987,
Pubmed
,
Xenbase
Danilchik,
Differentiation of the animal-vegetal axis in Xenopus laevis oocytes. I. Polarized intracellular translocation of platelets establishes the yolk gradient.
1987,
Pubmed
,
Xenbase
Darnbrough,
Identification in Xenopus laevis of a class of oocyte-specific proteins bound to messenger RNA.
1981,
Pubmed
,
Xenbase
Davis,
Selective dendritic transport of RNA in hippocampal neurons in culture.
,
Pubmed
Dreyfuss,
Structure and function of nuclear and cytoplasmic ribonucleoprotein particles.
1986,
Pubmed
Ephrussi,
Oskar organizes the germ plasm and directs localization of the posterior determinant nanos.
1991,
Pubmed
Frigerio,
Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network.
1986,
Pubmed
Garner,
Selective localization of messenger RNA for cytoskeletal protein MAP2 in dendrites.
1988,
Pubmed
Jeffery,
Spatial distribution of messenger RNA in the cytoskeletal framework of ascidian eggs.
1984,
Pubmed
Kim-Ha,
oskar mRNA is localized to the posterior pole of the Drosophila oocyte.
1991,
Pubmed
Konarska,
Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs.
1986,
Pubmed
Kondaiah,
Identification of a novel transforming growth factor-beta (TGF-beta 5) mRNA in Xenopus laevis.
1990,
Pubmed
,
Xenbase
Krieg,
Improved synthesis of full-length RNA probe at reduced incubation temperatures.
1990,
Pubmed
Lawrence,
Intracellular localization of messenger RNAs for cytoskeletal proteins.
1986,
Pubmed
Leibold,
Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5' untranslated region of ferritin heavy- and light-subunit mRNAs.
1988,
Pubmed
Macdonald,
cis-acting sequences responsible for anterior localization of bicoid mRNA in Drosophila embryos.
1988,
Pubmed
MacDonald,
bicoid mRNA localization signal: phylogenetic conservation of function and RNA secondary structure.
1990,
Pubmed
Marciniak,
Identification and characterization of a HeLa nuclear protein that specifically binds to the trans-activation-response (TAR) element of human immunodeficiency virus.
1990,
Pubmed
Melton,
Translocation of a localized maternal mRNA to the vegetal pole of Xenopus oocytes.
,
Pubmed
,
Xenbase
Mowry,
Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Müllner,
A specific mRNA binding factor regulates the iron-dependent stability of cytoplasmic transferrin receptor mRNA.
1989,
Pubmed
Murray,
Sequence analysis of cytoplasmic mRNA-binding proteins of Xenopus oocytes identifies a family of RNA-binding proteins.
1992,
Pubmed
,
Xenbase
Pokrywka,
Microtubules mediate the localization of bicoid RNA during Drosophila oogenesis.
1991,
Pubmed
Pollock,
Subcellular localization of transcripts in Drosophila photoreceptor neurons: chaoptic mutants have an aberrant distribution.
1990,
Pubmed
Raff,
Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos.
1990,
Pubmed
Rebagliati,
Identification and cloning of localized maternal RNAs from Xenopus eggs.
1985,
Pubmed
,
Xenbase
Wang,
Nanos is the localized posterior determinant in Drosophila.
1991,
Pubmed
Weeks,
A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta.
1987,
Pubmed
,
Xenbase
Whitfield,
Transcripts of one of two Drosophila cyclin genes become localized in pole cells during embryogenesis.
1989,
Pubmed
,
Xenbase
Wolffe,
The Y-box factors: a family of nucleic acid binding proteins conserved from Escherichia coli to man.
1992,
Pubmed
Wylie,
The cytoskeleton of Xenopus oocytes and its role in development.
1985,
Pubmed
,
Xenbase
Yisraeli,
The material mRNA Vg1 is correctly localized following injection into Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Yisraeli,
A two-step model for the localization of maternal mRNA in Xenopus oocytes: involvement of microtubules and microfilaments in the translocation and anchoring of Vg1 mRNA.
1990,
Pubmed
,
Xenbase
Yisraeli,
Synthesis of long, capped transcripts in vitro by SP6 and T7 RNA polymerases.
1989,
Pubmed
