Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1992 Dec 01;1212:5485-98. doi: 10.1128/mcb.12.12.5485-5498.1992.
Show Gene links
Show Anatomy links
Biochemical and cytological changes associated with expression of deregulated pp60src in Xenopus oocytes.
Unger TF
,
Steele RE
.
???displayArticle.abstract???
We have examined the effects of Xenopus pp60c-src with constitutive kinase activity on the morphology and maturation of Xenopus laevis oocytes. When RNA encoding this deregulated variant was injected into stage VI oocytes, we observed a gross alteration in the cortex of the oocyte. This alteration involved aggregation of pigment and invagination of the cortex in a large area proximal to the site of injection. This phenomenon was not seen in oocytes injected with RNA encoding wild-type pp60c-src. We have correlated this phenomenon with the tyrosine phosphorylation of 84- and 100-kDa proteins. These phosphorylated proteins colocalized with the alteration in the oocyte cortex when assayed by both biochemical and immunocytochemical methods. Neither the pigment aggregation nor phosphorylation of the 84- and 100-kDa proteins was observed in oocytes expressing a nonmyristoylated version of the deregulated pp60c-src. Expression of deregulated Xenopus fyn, a src-family member, resulted in a phenotype similar to that seen with deregulated src. However, in the fyn-injected oocytes, many more proteins were phosphorylated on tyrosine than in the src-injected oocytes. Progesterone stimulation of oocytes expressing deregulated pp60c-src resulted in an increase in the number of tyrosine-phosphorylated proteins. This change may represent the response of pp60src to the resumption of the cell cycle in maturing oocytes. These data suggest that the oocyte may be a particularly useful system for investigating the role of pp60c-src in the regulation of cytoskeletal structure and in the regulation of events associated with the cell cycle.
Alemà,
Differentiation of PC12 phaeochromocytoma cells induced by v-src oncogene.
, Pubmed
Alemà,
Differentiation of PC12 phaeochromocytoma cells induced by v-src oncogene.
,
Pubmed
Bement,
Transformation of the amphibian oocyte into the egg: structural and biochemical events.
1990,
Pubmed
,
Xenbase
Brown,
The stimulation of pp60v-src kinase activity by vanadate in intact cells accompanies a new phosphorylation state of the enzyme.
1984,
Pubmed
Buss,
Myristic acid is attached to the transforming protein of Rous sarcoma virus during or immediately after synthesis and is present in both soluble and membrane-bound forms of the protein.
1984,
Pubmed
Buss,
The absence of myristic acid decreases membrane binding of p60src but does not affect tyrosine protein kinase activity.
1986,
Pubmed
Carley,
F-actin aggregates in transformed cells.
1981,
Pubmed
Chackalaparampil,
Altered phosphorylation and activation of pp60c-src during fibroblast mitosis.
1988,
Pubmed
Chu,
Electroporation for the efficient transfection of mammalian cells with DNA.
1987,
Pubmed
Colman,
Fate of secretory proteins trapped in oocytes of Xenopus laevis by disruption of the cytoskeleton or by imbalanced subunit synthesis.
1981,
Pubmed
,
Xenbase
Cooper,
Related proteins are phosphorylated at tyrosine in response to mitogenic stimuli and at meiosis.
1989,
Pubmed
,
Xenbase
Cooper,
Dephosphorylation or antibody binding to the carboxy terminus stimulates pp60c-src.
1986,
Pubmed
Cooper,
Identification and characterization of cellular targets for tyrosine protein kinases.
1983,
Pubmed
Cooper,
Tyr527 is phosphorylated in pp60c-src: implications for regulation.
1986,
Pubmed
Cotton,
Neural tissues express high levels of the cellular src gene product pp60c-src.
1983,
Pubmed
Courtneidge,
Activation of the pp60c-src kinase by middle T antigen binding or by dephosphorylation.
1985,
Pubmed
Cross,
A short sequence in the p60src N terminus is required for p60src myristylation and membrane association and for cell transformation.
1984,
Pubmed
David-Pfeuty,
Immunolocalization of the cellular src protein in interphase and mitotic NIH c-src overexpresser cells.
1990,
Pubmed
DeClue,
Phosphorylation of talin at tyrosine in Rous sarcoma virus-transformed cells.
1987,
Pubmed
DeLorbe,
Molecular cloning and characterization of avian sarcoma virus circular DNA molecules.
1980,
Pubmed
Drubin,
Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I.
1990,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Elinson,
Cytoskeleton in Xenopus oocytes and eggs.
1990,
Pubmed
,
Xenbase
Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase
Fiszman,
Temperature-sensitive expression of differentiation in transformed myoblasts.
1975,
Pubmed
Gard,
Organization, nucleation, and acetylation of microtubules in Xenopus laevis oocytes: a study by confocal immunofluorescence microscopy.
1991,
Pubmed
,
Xenbase
Golden,
Blood platelets express high levels of the pp60c-src-specific tyrosine kinase activity.
1986,
Pubmed
Gorman,
The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.
1982,
Pubmed
Gould,
The protein-tyrosine kinase substrate, p81, is homologous to a chicken microvillar core protein.
1986,
Pubmed
Guarente,
A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.
1982,
Pubmed
Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Hamaguchi,
A glycoprotein in the plasma membrane matrix as a major potential substrate of p60v-src.
1990,
Pubmed
Hamaguchi,
Phosphorylation of cellular proteins in Rous sarcoma virus-infected cells: analysis by use of anti-phosphotyrosine antibodies.
1988,
Pubmed
Hirai,
Mutations in src homology regions 2 and 3 of activated chicken c-src that result in preferential transformation of mouse or chicken cells.
1990,
Pubmed
Hirai,
SH2 mutants of c-src that are host dependent for transformation are trans-dominant inhibitors of mouse cell transformation by activated c-src.
1990,
Pubmed
Holtzer,
Effect of oncogenic virus on muscle differentiation.
1975,
Pubmed
Iba,
Rous sarcoma virus variants that carry the cellular src gene instead of the viral src gene cannot transform chicken embryo fibroblasts.
1984,
Pubmed
Johnson,
Overexpressed pp60c-src can induce focus formation without complete transformation of NIH 3T3 cells.
1985,
Pubmed
Jove,
Cell transformation by the viral src oncogene.
1987,
Pubmed
Kamps,
Identification of multiple novel polypeptide substrates of the v-src, v-yes, v-fps, v-ros, and v-erb-B oncogenic tyrosine protein kinases utilizing antisera against phosphotyrosine.
1988,
Pubmed
Kamps,
Rous sarcoma virus transforming protein lacking myristic acid phosphorylates known polypeptide substrates without inducing transformation.
1986,
Pubmed
Kamps,
Mutation of NH2-terminal glycine of p60src prevents both myristoylation and morphological transformation.
1985,
Pubmed
Kawakami,
Acquisition of transforming properties by FYN, a normal SRC-related human gene.
1988,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Levy,
The structurally distinct form of pp60c-src detected in neuronal cells is encoded by a unique c-src mRNA.
1987,
Pubmed
Lohka,
Metaphase protein phosphorylation in Xenopus laevis eggs.
1987,
Pubmed
,
Xenbase
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Morgan,
Mitosis-specific phosphorylation of p60c-src by p34cdc2-associated protein kinase.
1989,
Pubmed
,
Xenbase
Moss,
Viral transformation of chick myogenic cells. The relationship between differentiation and the expression of the SRC gene.
1979,
Pubmed
Oppermann,
Uninfected vertebrate cells contain a protein that is closely related to the product of the avian sarcoma virus transforming gene (src).
1979,
Pubmed
Pacifici,
Transformation of chondroblasts by Rous sarcoma virus and synthesis of the sulfated proteoglycan matrix.
1977,
Pubmed
Parker,
Expression of v-src and chicken c-src in rat cells demonstrates qualitative differences between pp60v-src and pp60c-src.
1984,
Pubmed
Pasquale,
Talin is phosphorylated on tyrosine in chicken embryo fibroblasts transformed by Rous sarcoma virus.
1986,
Pubmed
Patschinsky,
Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.
1982,
Pubmed
Pawson,
Non-catalytic domains of cytoplasmic protein-tyrosine kinases: regulatory elements in signal transduction.
1988,
Pubmed
Rendu,
High pp60c-src level in human platelet dense bodies.
1989,
Pubmed
Reynolds,
Stable association of activated pp60src with two tyrosine-phosphorylated cellular proteins.
1989,
Pubmed
Reynolds,
Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene.
1989,
Pubmed
Roby,
Effects of Rous Sarcoma Virus on the synthetic programs of chondroblasts and retinal melanoblasts.
1976,
Pubmed
Rohrschneider,
Immunofluorescence on avian sarcoma virus-transformed cells: localization of the src gene product.
1979,
Pubmed
Sefton,
Vinculin: a cytoskeletal target of the transforming protein of Rous sarcoma virus.
1981,
Pubmed
Shalloway,
Overexpression of the c-src protein does not induce transformation of NIH 3T3 cells.
1984,
Pubmed
Shenoy,
Purified maturation promoting factor phosphorylates pp60c-src at the sites phosphorylated during fibroblast mitosis.
1989,
Pubmed
,
Xenbase
Smart,
Characterization of sites for tyrosine phosphorylation in the transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homologue (pp60c-src).
1981,
Pubmed
Spivack,
Microinjection of pp60v-src into Xenopus oocytes increases phosphorylation of ribosomal protein S6 and accelerates the rate of progesterone-induced meiotic maturation.
1984,
Pubmed
,
Xenbase
Steele,
Structure and expression of fyn genes in Xenopus laevis.
1990,
Pubmed
,
Xenbase
Steele,
The two Xenopus laevis SRC genes are co-expressed and each produces functional pp60src.
1989,
Pubmed
,
Xenbase
Steele,
Two divergent cellular src genes are expressed in Xenopus laevis.
1985,
Pubmed
,
Xenbase
Wu,
Identification and characterization of a novel cytoskeleton-associated pp60src substrate.
1991,
Pubmed
Wylie,
The cytoskeleton of Xenopus oocytes and its role in development.
1985,
Pubmed
,
Xenbase
