Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70.
White TW
,
Bruzzone R
,
Goodenough DA
,
Paul DL
.
???displayArticle.abstract???
The crystalline lens is an attractive system to study the biology of intercellular communication; however, the identity of the structural components of gap junctions in the lens has been controversial. We have cloned a novel member of the connexin family of gap junction proteins, Cx50, and have shown that it is likely to correspond to the previously described lens fiber protein MP70. The N-terminal amino acid sequence of MP70 closely matches the sequence predicted by the clone. Cx50 mRNA is detected only in the lens, among the 12 organs tested, and this distribution is indistinguishable from that of MP70 protein. A monoclonal antibody directed against MP70 and an anti-Cx50 antibody produced against a synthetic peptide identify the same proteins on western blots and produce identical patterns of immunofluorescence on frozen sections of rodent lens. We also show that expression of Cx50 in paired Xenopus oocytes induces high levels of voltage-dependent conductance. This indicates that Cx50 is a functional member of the connexin family with unique physiological properties. With the cloning of Cx50, all known participants in gap junction formation between various cell types in the lens are available for study and reconstitution in experimental systems.
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991, Pubmed,
Xenbase
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991,
Pubmed
,
Xenbase
Bassnett,
The influence of pH on membrane conductance and intercellular resistance in the rat lens.
1988,
Pubmed
Benedetti,
Development of junctions during differentiation of lens fibers.
1974,
Pubmed
Benedetti,
A portrait of plasma membrane specializations in eye lens epithelium and fibers.
1976,
Pubmed
Bennett,
Gap junctions: new tools, new answers, new questions.
1991,
Pubmed
Beyer,
Antisera directed against connexin43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues.
1989,
Pubmed
Beyer,
Connexin family of gap junction proteins.
1990,
Pubmed
Beyer,
Connexin43: a protein from rat heart homologous to a gap junction protein from liver.
1987,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Dahl,
Expression of functional cell-cell channels from cloned rat liver gap junction complementary DNA.
1987,
Pubmed
,
Xenbase
Ebihara,
Cloning and expression of a Xenopus embryonic gap junction protein.
1989,
Pubmed
,
Xenbase
Emptage,
Internal acidification modulates membrane and junctional resistance in the isolated lens of the frog Rana pipiens.
1992,
Pubmed
Goodenough,
Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions.
1988,
Pubmed
Goodenough,
Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with freeze-substitution autoradiography and electron microscopy.
1980,
Pubmed
Goodenough,
Lens gap junctions: a structural hypothesis for nonregulated low-resistance intercellular pathways.
1979,
Pubmed
Green,
Analysis of the rat liver gap junction protein: clarification of anomalies in its molecular size.
1988,
Pubmed
Gruijters,
Immunolocalization of MP70 in lens fiber 16-17-nm intercellular junctions.
1987,
Pubmed
Haefliger,
Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping.
1992,
Pubmed
Helms,
A new method for purifying lambda DNA from phage lysates.
1985,
Pubmed
Kistler,
Homologies between gap junction proteins in lens, heart and liver.
1988,
Pubmed
Kistler,
Protein processing in lens intercellular junctions: cleavage of MP70 to MP38.
1987,
Pubmed
Kistler,
Identification of a 70,000-D protein in lens membrane junctional domains.
1985,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Laird,
Turnover and phosphorylation dynamics of connexin43 gap junction protein in cultured cardiac myocytes.
1991,
Pubmed
Mathias,
Cell to cell communication and pH in the frog lens.
1991,
Pubmed
Miller,
Structure of a gap junction gene: rat connexin-32.
1988,
Pubmed
Miller,
Evidence for two physiologically distinct gap junctions expressed by the chick lens epithelial cell.
1986,
Pubmed
Musil,
Expression of the gap junction protein connexin43 in embryonic chick lens: molecular cloning, ultrastructural localization, and post-translational phosphorylation.
1990,
Pubmed
Paul,
Molecular cloning of cDNA for rat liver gap junction protein.
1986,
Pubmed
Paul,
Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Peracchia,
Calcium effects on gap junction structure and cell coupling.
1978,
Pubmed
Rae,
The electrophysiology of the crystalline lens.
1979,
Pubmed
Rae,
The electrical coupling of epithelium and fibers in the frog lens.
1983,
Pubmed
Schuetze,
Dye transfer between cells of the embryonic chick lens becomes less sensitive to CO2 treatment with development.
1982,
Pubmed
Swenson,
Formation of gap junctions by expression of connexins in Xenopus oocyte pairs.
1989,
Pubmed
,
Xenbase
Zhang,
Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA.
1989,
Pubmed
