Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Mol Evol
1992 Jun 01;346:478-92. doi: 10.1007/bf00160462.
Show Gene links
Show Anatomy links
The boll weevil vitellogenin gene: nucleotide sequence, structure, and evolutionary relationship to nematode and vertebrate vitellogenin genes.
Trewitt PM
,
Heilmann LJ
,
Degrugillier SS
,
Kumaran AK
.
???displayArticle.abstract???
Boll weevil (Anthonomus grandis) eggs contain two yolk proteins, YP47 and YP160. Using anti-YP160 antiserum as probe, a partial-length complementary DNA (cDNA) was isolated from a lambda gt11 adult female cDNA library. A second partial-length cDNA was isolated from a lambda gt10 adult female cDNA library by differential screening with male vs. female cDNAs. Northern blot analysis showed that each cloned cDNA hybridized to a 6-kb female-specific transcript. These cDNAs were used to probe a genomic library, and two overlapping genomic clones were obtained that span the boll weevil vitellogenin gene. The entire transcription unit was sequenced, and introns were mapped by a combination of primer extension experiments, S1 nuclease protection experiments, and polymerase chain reaction-mediated synthesis of two additional cDNA clones. Based on these data, the vitellogenin mRNA is 5511 nucleotides [plus a poly(A) tail of undetermined length] and specifies a provitellogenin of 1790 amino acids. The deduced protein has a Glu+Gln content of 16.3%, which is a relatively high value that is typical of most vitellogenins. Protein sequence similarities including Cys clusters conserved between boll weevil vitellogenin and Xenopus laevis A2 or Caenorhabditis elegans vit-5 vitellogenins indicated that the boll weevil protein is a member of the ancient nematode-vertebrate vitellogenin family. Moreover, the six introns in the boll weevil vitellogenin gene interrupt the coding region at positions closely or exactly corresponding to a subset of the positions of the 34 vertebrate vitellogenin introns, further supporting the argument for a common evolutionary relationship. This report represents the first complete nucleotide sequence and structural analysis of a nondipteran insect vitellogenin gene.
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977, Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Bingham,
On/off regulation of gene expression at the level of splicing.
1988,
Pubmed
Bownes,
Evidence that insect embryogenesis is regulated by ecdysteroids released from yolk proteins.
1988,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Breathnach,
Organization and expression of eucaryotic split genes coding for proteins.
1981,
Pubmed
Byrne,
The evolution of egg yolk proteins.
1989,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Church,
Genomic sequencing.
1984,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Dhadialla,
Biosynthesis of mosquito vitellogenin.
1990,
Pubmed
Doolittle,
Searching through sequence databases.
1990,
Pubmed
Garabedian,
The nucleotide sequence of the gene coding for Drosophila melanogaster yolk protein 3.
1987,
Pubmed
Gerber-Huber,
Precursor-product relationship between vitellogenin and the yolk proteins as derived from the complete sequence of a Xenopus vitellogenin gene.
1987,
Pubmed
,
Xenbase
Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed
Harnish,
An evolutionary model for the insect vitellins.
1982,
Pubmed
Henikoff,
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
1984,
Pubmed
Hovemann,
Vitellogenin in Drosophila melanogaster: sequence of the yolk protein I gene and its flanking regions.
1981,
Pubmed
Hung,
Sequence and structure conservation in yolk proteins and their genes.
1983,
Pubmed
Jendrisak,
Cloning cDNA into lambda gt10 and lambda gt11.
1987,
Pubmed
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lehrach,
RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
1977,
Pubmed
Locke,
Cloning and 5' end nucleotide sequences of two juvenile hormone-inducible vitellogenin genes of the African migratory locust.
1987,
Pubmed
Maitre,
[Characterization of specific receptors for estradiol, induction of vitellogenin and its mRNA in the liver of rainbow trout (Salmo gairdnerii)].
1985,
Pubmed
Nardelli,
Vertebrate and nematode genes coding for yolk proteins are derived from a common ancestor.
1987,
Pubmed
,
Xenbase
Rina,
A cluster of vitellogenin genes in the Mediterranean fruit fly Ceratitis capitata: sequence and structural conservation in dipteran yolk proteins and their genes.
1991,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sharrock,
Cleavage of two yolk proteins from a precursor in Caenorhabditis elegans.
1984,
Pubmed
Shyu,
Expression of the vitellogenin gene in female and male sea urchin.
1986,
Pubmed
Shyu,
A single gene encoding vitellogenin in the sea urchin Strongylocentrotus purpuratus: sequence at the 5' end.
1987,
Pubmed
Singh,
The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures.
1984,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Spieth,
Vitellogenin motifs conserved in nematodes and vertebrates.
1991,
Pubmed
,
Xenbase
Spieth,
The Caenorhabditis elegans vitellogenin gene family includes a gene encoding a distantly related protein.
1985,
Pubmed
Spieth,
The nucleotide sequence of a nematode vitellogenin gene.
1985,
Pubmed
Terpstra,
Homology of Drosophila yolk proteins and the triacylglycerol lipase family.
1988,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Trewitt,
Multiple clustered genes of the haemoglobin VIIB subfamily of Chironomus thummi thummi (Diptera).
1988,
Pubmed
von Heijne,
A new method for predicting signal sequence cleavage sites.
1986,
Pubmed
Wahli,
Xenopus vitellogenin genes.
1985,
Pubmed
,
Xenbase
Wahli,
Evolution and expression of vitellogenin genes.
1988,
Pubmed
Wang,
Biosynthesis of the vitellogenins. Identification and characterization of nonphosphorylated precursors to avian vitellogenin I and vitellogenin II.
1982,
Pubmed
Wheeler,
Purification and characterization of honey bee vitellogenin.
1990,
Pubmed
Yamamoto,
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
1970,
Pubmed
Yan,
Sequence homologies among the three yolk polypeptide (Yp) genes in Drosophila melanogaster.
1987,
Pubmed
