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J Virol
2005 Feb 01;794:2356-65. doi: 10.1128/JVI.79.4.2356-2365.2005.
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Structural and functional analysis of the RNA transport element, a member of an extensive family present in the mouse genome.
Smulevitch S
,
Michalowski D
,
Zolotukhin AS
,
Schneider R
,
Bear J
,
Roth P
,
Pavlakis GN
,
Felber BK
.
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We previously identified an RNA transport element (RTE), present in a subclass of rodent intracisternal A particle retroelements (F. Nappi, R. Schneider, A. Zolotukhin, S. Smulevitch, D. Michalowski, J. Bear, B. Felber, and G. Pavlakis, J. Virol. 75:4558-4569, 2001), that is able to replace Rev-responsive element regulation in human immunodeficiency virus type 1. RTE-directed mRNA export is mediated by a still-unknown cellular factor(s), is independent of the CRM1 nuclear export receptor, and is conserved among vertebrates. Here we show that this RTE folds into an extended RNA secondary structure and thus does not resemble any known RTEs. Computer searches revealed the presence of 105 identical elements and more than 3,000 related elements which share at least 70% sequence identity with the RTE and which are found on all mouse chromosomes. These related elements are predicted to fold into RTE-like structures. Comparison of the sequences and structures revealed that the RTE and related elements can be divided into four groups. Mutagenesis of the RTE revealed that the minimal element contains four internal stem-loops, which are indispensable for function in mammalian cells. In contrast, only part of the element is essential to mediate RNA transport in microinjected Xenopus laevis oocyte nuclei. Importantly, the minimal RTE able to promote RNA transport has key structural features which are preserved in all the RTE-related elements, further supporting their functional importance. Therefore, RTE function depends on a complex secondary structure that is important for the interaction with the cellular export factor(s).
Ahmed,
Structure-function analyses of the HTLV-I Rex and HIV-1 Rev RNA response elements: insights into the mechanism of Rex and Rev action.
1990, Pubmed
Ahmed,
Structure-function analyses of the HTLV-I Rex and HIV-1 Rev RNA response elements: insights into the mechanism of Rex and Rev action.
1990,
Pubmed
Bogerd,
Mutational definition of functional domains within the Rev homolog encoded by human endogenous retrovirus K.
2000,
Pubmed
Braun,
TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus.
1999,
Pubmed
,
Xenbase
Bray,
A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent.
1994,
Pubmed
Ciesiołka,
Patterns of cleavages induced by lead ions in defined RNA secondary structure motifs.
1998,
Pubmed
Conti,
Nucleocytoplasmic transport enters the atomic age.
2001,
Pubmed
Dewannieux,
Identification of autonomous IAP LTR retrotransposons mobile in mammalian cells.
2004,
Pubmed
Erkmann,
Nuclear export of mRNA: from the site of transcription to the cytoplasm.
2004,
Pubmed
Ernst,
A structured retroviral RNA element that mediates nucleocytoplasmic export of intron-containing RNA.
1997,
Pubmed
Ernst,
Secondary structure and mutational analysis of the Mason-Pfizer monkey virus RNA constitutive transport element.
1997,
Pubmed
Felber,
rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA.
1989,
Pubmed
Felber,
Feedback regulation of human immunodeficiency virus type 1 expression by the Rev protein.
1990,
Pubmed
Grüter,
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.
1998,
Pubmed
,
Xenbase
Görlich,
Transport between the cell nucleus and the cytoplasm.
1999,
Pubmed
Hadzopoulou-Cladaras,
The rev (trs/art) protein of human immunodeficiency virus type 1 affects viral mRNA and protein expression via a cis-acting sequence in the env region.
1989,
Pubmed
Herold,
Genome-wide analysis of nuclear mRNA export pathways in Drosophila.
2003,
Pubmed
Izaurralde,
Transport of macromolecules between the nucleus and the cytoplasm.
1998,
Pubmed
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Löwer,
Identification of a Rev-related protein by analysis of spliced transcripts of the human endogenous retroviruses HTDV/HERV-K.
1995,
Pubmed
Magin,
cORF and RcRE, the Rev/Rex and RRE/RxRE homologues of the human endogenous retrovirus family HTDV/HERV-K.
1999,
Pubmed
Malim,
The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA.
1989,
Pubmed
Nappi,
Identification of a novel posttranscriptional regulatory element by using a rev- and RRE-mutated human immunodeficiency virus type 1 DNA proviral clone as a molecular trap.
2001,
Pubmed
,
Xenbase
Nasioulas,
Production of avian leukosis virus particles in mammalian cells can be mediated by the interaction of the human immunodeficiency virus protein Rev and the Rev-responsive element.
1995,
Pubmed
Ogert,
Avian retroviral RNA element promotes unspliced RNA accumulation in the cytoplasm.
1996,
Pubmed
Ogert,
Mutational analysis of the rous sarcoma virus DR posttranscriptional control element.
1998,
Pubmed
Paca,
Rous sarcoma virus DR posttranscriptional elements use a novel RNA export pathway.
2000,
Pubmed
Saavedra,
The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export.
1997,
Pubmed
,
Xenbase
Schneider,
Inactivation of the human immunodeficiency virus type 1 inhibitory elements allows Rev-independent expression of Gag and Gag/protease and particle formation.
1997,
Pubmed
Segref,
Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores.
1997,
Pubmed
Solomin,
Different sites of interaction for Rev, Tev, and Rex proteins within the Rev-responsive element of human immunodeficiency virus type 1.
1990,
Pubmed
Stauber,
Development and applications of enhanced green fluorescent protein mutants.
1998,
Pubmed
Tabernero,
The posttranscriptional control element of the simian retrovirus type 1 forms an extensive RNA secondary structure necessary for its function.
1996,
Pubmed
Tabernero,
Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1.
1997,
Pubmed
Tan,
The mRNA export in Caenorhabditis elegans is mediated by Ce-NXF-1, an ortholog of human TAP/NXF and Saccharomyces cerevisiae Mex67p.
2000,
Pubmed
Yang,
The human endogenous retrovirus K Rev response element coincides with a predicted RNA folding region.
2000,
Pubmed
Zolotukhin,
Continuous propagation of RRE(-) and Rev(-)RRE(-) human immunodeficiency virus type 1 molecular clones containing a cis-acting element of simian retrovirus type 1 in human peripheral blood lymphocytes.
1994,
Pubmed
Zolotukhin,
Retroviral constitutive transport element evolved from cellular TAP(NXF1)-binding sequences.
2001,
Pubmed
Zolotukhin,
U2AF participates in the binding of TAP (NXF1) to mRNA.
2002,
Pubmed