XB-ART-24136
Mol Reprod Dev
1992 Jan 01;311:48-54. doi: 10.1002/mrd.1080310109.
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The main adenosine triphosphate-binding component of amphibian oocytes is ferritin.
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After fractionation of mitochondrion-free extracts of Xenopus laevis and Rana temporaria oocytes in sucrose gradients, a distinct peak of adenosine triphosphate (ATP)/guanosine triphosphate (GTP)-binding activity in the 50-70 S range has been detected. This substance has a boyant density in Cs2SO4 of 1.45 g/cm3. The nucleotide-binding substance has been purified to apparent homogenety. By means of electron microscopy, sodium dodecyl sulfate-electrophoresis and other methods it has been identified as ferritin.
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