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Proc Natl Acad Sci U S A
1991 Sep 01;8817:7679-83.
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Two nonallelic insulin genes in Xenopus laevis are expressed differentially during neurulation in prepancreatic embryos.
Shuldiner AR
,
de Pablo F
,
Moore CA
,
Roth J
.
???displayArticle.abstract??? Insulin, traditionally regarded as a metabolic hormone, also can potently stimulate growth and differentiation in many cell types. To study further the potential role of insulin during early embryogenesis, we have used the amphibian Xenopus laevis, a versatile model of vertebrate development. Using (i) nucleotide sequences of two previously cloned cDNAs that correspond to two different nonallelic Xenopus insulin genes (both of which are expressed in the adult pancreas) and (ii) a modification of the highly sensitive reverse transcription-polymerase chain reaction (RT-PCR) method developed in our laboratory, designated RNA template-specific PCR (RS-PCR), we now find that mRNAs for both Xenopus insulins I and II are present in mature (stage VI) oocytes but not in less-mature oocytes (stages I and IV) or in unfertilized eggs. The Xenopus insulin II gene is differentially expressed during early neurulation (stage 13), while only the insulin I gene is expressed at stage 21, when the neural tube is closing and cephalization is beginning. During later stages (i.e., stage 26) there is a region in the head that appears to be transcribing only the insulin I gene, while mRNAs for both insulins I and II are present in the body region. These findings show that the two nonallelic insulin genes are expressed differentially in Xenopus embryos in a stage- and region-specific manner; because appropriate receptors are also present, we suggest a role for insulin during early nervous system development well before the emergence of pancreatic beta cells.
Adamo,
Insulin and insulin-like growth factor receptors in the nervous system.
1989, Pubmed
Adamo,
Insulin and insulin-like growth factor receptors in the nervous system.
1989,
Pubmed
Alpert,
Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons.
1988,
Pubmed
Bach,
Alternative splicing produces messenger RNAs encoding insulin-like growth factor-I prohormones that are differentially glycosylated in vitro.
1990,
Pubmed
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
de Pablo,
Endocrinization of the early embryo: an emerging role for hormones and hormone-like factors.
1990,
Pubmed
de Pablo,
Insulin antibodies retard and insulin accelerates growth and differentiation in early embryos.
1985,
Pubmed
De Pablo,
Insulin is present in chicken eggs and early chick embryos.
1982,
Pubmed
Doe,
Expression and function of the segmentation gene fushi tarazu during Drosophila neurogenesis.
1988,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Freinkel,
Diabetic embryopathy and fuel-mediated organ teratogenesis: lessons from animal models.
1988,
Pubmed
Garofalo,
Tissue localization of Drosophila melanogaster insulin receptor transcripts during development.
1988,
Pubmed
Giddings,
Selective expression and developmental regulation of the ancestral rat insulin II gene in fetal liver.
1990,
Pubmed
Giebelhaus,
Changes in the expression of alpha-fodrin during embryonic development of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Havrankova,
Insulin receptors are widely distributed in the central nervous system of the rat.
1978,
Pubmed
Heidenreich,
Insulin receptors mediate growth effects in cultured fetal neurons. I. Rapid stimulation of protein synthesis.
1989,
Pubmed
Higuchi,
A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.
1988,
Pubmed
Janicot,
Activation of glucose uptake by insulin and insulin-like growth factor I in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Kawasaki,
Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro.
1988,
Pubmed
Kimelman,
Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
1987,
Pubmed
,
Xenbase
Klemsz,
The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene.
1990,
Pubmed
LeRoith,
Insulin-related materials in the nervous system of vertebrates and non-vertebrates: possible extrapancreatic production.
1988,
Pubmed
Mercola,
Platelet-derived growth factor A chain is maternally encoded in Xenopus embryos.
1988,
Pubmed
,
Xenbase
Miller,
Stimulation of protein synthesis in stage IV Xenopus oocytes by microinjected insulin.
1989,
Pubmed
,
Xenbase
Mills,
Lack of relation of increased malformation rates in infants of diabetic mothers to glycemic control during organogenesis.
1988,
Pubmed
Nagasawa,
Amino acid sequence of a prothoracicotropic hormone of the silkworm Bombyx mori.
1986,
Pubmed
Nagasawa,
Amino-terminal amino Acid sequence of the silkworm prothoracicotropic hormone: homology with insulin.
1984,
Pubmed
Perrimon,
Multiple functions of a Drosophila homeotic gene, zeste-white 3, during segmentation and neurogenesis.
1989,
Pubmed
Rappolee,
Wound macrophages express TGF-alpha and other growth factors in vivo: analysis by mRNA phenotyping.
1988,
Pubmed
Recio-Pinto,
Insulin and insulin-like growth factor II permit nerve growth factor binding and the neurite formation response in cultured human neuroblastoma cells.
1984,
Pubmed
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Scavo,
Genes encoding receptors for insulin and insulin-like growth factor I are expressed in Xenopus oocytes and embryos.
1991,
Pubmed
,
Xenbase
Serrano,
Insulin gene expression in chicken ontogeny: pancreatic, extrapancreatic, and prepancreatic.
1989,
Pubmed
Shuldiner,
Xenopus laevis contains two nonallelic preproinsulin genes. cDNA cloning and evolutionary perspective.
1989,
Pubmed
,
Xenbase
Shuldiner,
Isolation and characterization of two different insulins from an amphibian, Xenopus laevis.
1989,
Pubmed
,
Xenbase
Shuldiner,
RNA template-specific polymerase chain reaction (RS-PCR): a novel strategy to reduce dramatically false positives.
1990,
Pubmed
,
Xenbase
Smit,
Growth-controlling molluscan neurons produce the precursor of an insulin-related peptide.
1988,
Pubmed
Smith,
Identification of a potent Xenopus mesoderm-inducing factor as a homologue of activin A.
1990,
Pubmed
,
Xenbase
Sokol,
A mouse macrophage factor induces head structures and organizes a body axis in Xenopus.
1990,
Pubmed
,
Xenbase
Stefanovic,
Activation of a ribosomal protein S6 protein kinase in Xenopus oocytes by insulin and insulin-receptor kinase.
1986,
Pubmed
,
Xenbase
Stith,
The effect of insulin on intracellular ph and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Weeks,
A maternal mRNA localized to the vegetal hemisphere in Xenopus eggs codes for a growth factor related to TGF-beta.
1987,
Pubmed
,
Xenbase
Wharton,
Nucleotide sequence from the neurogenic locus notch implies a gene product that shares homology with proteins containing EGF-like repeats.
1985,
Pubmed
