Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1991 Jun 01;116:3115-24. doi: 10.1128/mcb.11.6.3115-3124.1991.
Show Gene links
Show Anatomy links
Degradation of a developmentally regulated mRNA in Xenopus embryos is controlled by the 3' region and requires the translation of another maternal mRNA.
???displayArticle.abstract???
By injecting the appropriately constructed plasmids into one-cell Xenopus embryos, we determined that the 3' region of the maternal Xenopus Eg2 mRNA confers instability on the chimeric mRNA transcribed from these plasmids. This instability, like that of the maternal Eg2 transcript, was abolished by treatment of the embryos with cycloheximide. Analysis of the polysome distribution of the maternal Eg2 mRNA in cycloheximide-treated and untreated embryos showed that Eg2 mRNA was released from polysomes after fertilization and that the stabilization caused by cycloheximide treatment was not due to a reloading of ribosomes onto the mRNA. Insertion of a stable hairpin loop (delta G = -50 kcal/mol) 5' to the reporter gene in the injected plasmid caused a 10- to 20-fold decrease in translation from the transcribed mRNAs. This decrease in translation did not abolish the instability conferred by the 3' Eg2 region. Therefore, the degradation of these chimeric mRNAs in Xenopus embryos requires the translation of another maternal mRNA coding for a trans-acting factor involved in mRNA degradation. Further restriction of the 3' Eg2 region, placed 3' to the reporter gene, showed that a cis-acting instability-conferring sequence is contained in a 497-nucleotide fragment.
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972, Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Bassez,
Post-transcriptional regulation of ornithine decarboxylase in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Bienz,
Xenopus hsp 70 genes are constitutively expressed in injected oocytes.
1984,
Pubmed
,
Xenbase
Brawerman,
The Role of the poly(A) sequence in mammalian messenger RNA.
1981,
Pubmed
,
Xenbase
Brawerman,
mRNA decay: finding the right targets.
1989,
Pubmed
Brewer,
Poly(A) shortening and degradation of the 3' A+U-rich sequences of human c-myc mRNA in a cell-free system.
1988,
Pubmed
Brewer,
Regulation of c-myc mRNA stability in vitro by a labile destabilizer with an essential nucleic acid component.
1989,
Pubmed
Duval,
Stability of maternal mRNA in Xenopus embryos: role of transcription and translation.
1990,
Pubmed
,
Xenbase
Etzerodt,
Structure and expression of a Xenopus gene encoding an snRNP protein (U1 70K).
1988,
Pubmed
,
Xenbase
Gimlich,
Differential regulation of the levels of three gap junction mRNAs in Xenopus embryos.
1990,
Pubmed
,
Xenbase
Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed
Gurdon,
Transcription of muscle-specific actin genes in early Xenopus development: nuclear transplantation and cell dissociation.
1984,
Pubmed
,
Xenbase
Harland,
Stability of RNA in developing Xenopus embryos and identification of a destabilizing sequence in TFIIIA messenger RNA.
1988,
Pubmed
,
Xenbase
Harland,
Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA.
1985,
Pubmed
,
Xenbase
Kelly,
Role of protein synthesis in decay and accumulation of mRNA during spore germination in the cellular slime mold Dictyostelium discoideum.
1987,
Pubmed
Koeller,
A cytosolic protein binds to structural elements within the iron regulatory region of the transferrin receptor mRNA.
1989,
Pubmed
Kozak,
Influences of mRNA secondary structure on initiation by eukaryotic ribosomes.
1986,
Pubmed
Malter,
Identification of an AUUUA-specific messenger RNA binding protein.
1989,
Pubmed
Müllner,
A specific mRNA binding factor regulates the iron-dependent stability of cytoplasmic transferrin receptor mRNA.
1989,
Pubmed
Müllner,
A stem-loop in the 3' untranslated region mediates iron-dependent regulation of transferrin receptor mRNA stability in the cytoplasm.
1988,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Paris,
Changes in the polyadenylation of specific stable RNA during the early development of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Paris,
Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development.
1990,
Pubmed
,
Xenbase
Paris,
Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2.
1991,
Pubmed
,
Xenbase
Parker,
Translation and a 42-nucleotide segment within the coding region of the mRNA encoded by the MAT alpha 1 gene are involved in promoting rapid mRNA decay in yeast.
1990,
Pubmed
Peltz,
Autogenous regulation of histone mRNA decay by histone proteins in a cell-free system.
1987,
Pubmed
Pierandrei-Amaldi,
Expression of ribosomal-protein genes in Xenopus laevis development.
1982,
Pubmed
,
Xenbase
Rebagliati,
Identification and cloning of localized maternal RNAs from Xenopus eggs.
1985,
Pubmed
,
Xenbase
Ruiz i Altaba,
Xfin: an embryonic gene encoding a multifingered protein in Xenopus.
1987,
Pubmed
,
Xenbase
Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Shaw,
A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
1986,
Pubmed
Shyu,
The c-fos transcript is targeted for rapid decay by two distinct mRNA degradation pathways.
1989,
Pubmed
Smith,
Destruction of a translationally controlled mRNA in Xenopus oocytes delays progesterone-induced maturation.
1988,
Pubmed
,
Xenbase
Steel,
Translational control of ribosomal protein synthesis during early Dictyostelium discoideum development.
1987,
Pubmed
Stick,
cDNA cloning of the developmentally regulated lamin LIII of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Treisman,
Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences.
1985,
Pubmed
Wilson,
Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3' AU-rich sequences.
1988,
Pubmed
Yen,
Autoregulated instability of beta-tubulin mRNAs by recognition of the nascent amino terminus of beta-tubulin.
1988,
Pubmed
