Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1991 Jan 01;111:47-54. doi: 10.1128/mcb.11.1.47-54.1991.
Show Gene links
Show Anatomy links
Retrovirus-induced interference with collagen I gene expression in Mov13 fibroblasts is maintained in the absence of DNA methylation.
Chan H
,
Hartung S
,
Breindl M
.
???displayArticle.abstract???
We have studied the role of DNA methylation in repression of the murine alpha 1 type I collagen (COL1A1) gene in Mov13 fibroblasts. In Mov13 mice, a retroviral provirus has inserted into the first intron of the COL1A1 gene and blocks its expression at the level of transcriptional initiation. We found that regulatory sequences in the COL1A1 promoter region that are involved in the tissue-specific regulation of the gene are unmethylated in collagen-expressing wild-type fibroblasts and methylated in Mov13 fibroblasts, confirming and extending earlier observations. To directly assess the role of DNA methylation in the repression of COL1A1 gene transcription, we treated Mov13 fibroblasts with the demethylating agent 5-azacytidine. This treatment resulted in a demethylation of the COL1A1 regulatory sequences but failed to activate transcription of the COL1A1 gene. Moreover, the 5-azacytidine treatment induced a transcription-competent chromatin structure in the retroviral sequences but not in the COL1A1 promoter. In DNA transfection and microinjection experiments, we found that the provirus interfered with transcriptional activity of the COL1A1 promoter in Mov13 fibroblasts but not in Xenopus laevis oocytes. In contrast, the wild-type COL1A1 promoter was transcriptionally active in Mov13 fibroblasts. These experiments showed that the COL1A1 promoter is potentially transcriptionally active in the presence of proviral sequences and that Mov13 fibroblasts contain the trans-acting factors required for efficient COL1A1 gene expression. Our results indicate that the provirus insertion in Mov13 can inactivate COL1A1 gene expression at several levels. It prevents the developmentally regulated establishment of a transcription-competent methylation pattern and chromatin structure of the COL1A1 domain and, in the absence of DNA methylation, appears to suppress the COL1A1 promoter in a cell-specific manner, presumably by assuming a dominant chromatin structure that may be incompatible with transcriptional activity of flanking cellular sequences.
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980, Pubmed
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980,
Pubmed
Bornstein,
Regulatory elements in the first intron contribute to transcriptional control of the human alpha 1(I) collagen gene.
1987,
Pubmed
Bornstein,
Interactions between the promoter and first intron are involved in transcriptional control of alpha 1(I) collagen gene expression.
1988,
Pubmed
Bornstein,
The first intron of the alpha 1(I) collagen gene contains several transcriptional regulatory elements.
1988,
Pubmed
Breindl,
DNase I sensitivity of endogenous and exogenous proviral genome copies in M-MuLV-induced tumors of Mov-3 Mice.
1982,
Pubmed
Breindl,
Retrovirus-induced lethal mutation in collagen I gene of mice is associated with an altered chromatin structure.
1984,
Pubmed
Brenner,
Analysis of the collagen alpha 1(I) promoter.
1989,
Pubmed
Buschhausen,
Chromatin structure is required to block transcription of the methylated herpes simplex virus thymidine kinase gene.
1987,
Pubmed
Cedar,
DNA methylation and gene activity.
1988,
Pubmed
Chu,
Fine structural analysis of the human pro-alpha 1 (I) collagen gene. Promoter structure, AluI repeats, and polymorphic transcripts.
1985,
Pubmed
Cullen,
Transcriptional interference in avian retroviruses--implications for the promoter insertion model of leukaemogenesis.
,
Pubmed
Doerfler,
DNA methylation and gene activity.
1983,
Pubmed
Emerman,
Genes with promoters in retrovirus vectors can be independently suppressed by an epigenetic mechanism.
1984,
Pubmed
Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed
Harbers,
Insertion of retrovirus into the first intron of alpha 1(I) collagen gene to embryonic lethal mutation in mice.
1984,
Pubmed
Harbers,
DNA methylation and gene expression: endogenous retroviral genome becomes infectious after molecular cloning.
1981,
Pubmed
Hartung,
Retrovirus insertion inactivates mouse alpha 1(I) collagen gene by blocking initiation of transcription.
,
Pubmed
Hayward,
Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis.
1981,
Pubmed
Horowitz,
Mechanism of activation of the mouse c-mos oncogene by the LTR of an intracisternal A-particle gene.
1984,
Pubmed
Jaenisch,
Treatment of mice with 5-azacytidine efficiently activates silent retroviral genomes in different tissues.
1985,
Pubmed
Jaenisch,
Retroviruses and insertional mutagenesis.
1985,
Pubmed
Jaenisch,
Germline integration of moloney murine leukemia virus at the Mov13 locus leads to recessive lethal mutation and early embryonic death.
1983,
Pubmed
Jähner,
Retrovirus-induced de novo methylation of flanking host sequences correlates with gene inactivity.
,
Pubmed
Karsenty,
Point mutations and small substitution mutations in three different upstream elements inhibit the activity of the mouse alpha 2(I) collagen promoter.
1988,
Pubmed
Keshet,
DNA methylation affects the formation of active chromatin.
1986,
Pubmed
Kratochwil,
Retrovirus-induced insertional mutation in Mov13 mice affects collagen I expression in a tissue-specific manner.
1989,
Pubmed
Lichtler,
Isolation and characterization of the rat alpha 1(I) collagen promoter. Regulation by 1,25-dihydroxyvitamin D.
1989,
Pubmed
McBurney,
Isolation of male embryonal carcinoma cells and their chromosome replication patterns.
1982,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Nusse,
Mode of proviral activation of a putative mammary oncogene (int-1) on mouse chromosome 15.
,
Pubmed
Proudfoot,
Transcriptional interference and termination between duplicated alpha-globin gene constructs suggests a novel mechanism for gene regulation.
,
Pubmed
Rasmussen,
Significance of DNase I-hypersensitive sites in the long terminal repeats of a Moloney murine leukemia virus vector.
1987,
Pubmed
Rippe,
Regulatory elements in the 5'-flanking region and the first intron contribute to transcriptional control of the mouse alpha 1 type I collagen gene.
1989,
Pubmed
Rossouw,
DNA sequences in the first intron of the human pro-alpha 1(I) collagen gene enhance transcription.
1987,
Pubmed
,
Xenbase
Schnieke,
Introduction of the human pro alpha 1(I) collagen gene into pro alpha 1(I)-deficient Mov-13 mouse cells leads to formation of functional mouse-human hybrid type I collagen.
1987,
Pubmed
Schnieke,
Embryonic lethal mutation in mice induced by retrovirus insertion into the alpha 1(I) collagen gene.
,
Pubmed
Selten,
Proviral activation of the putative oncogene Pim-1 in MuLV induced T-cell lymphomas.
1985,
Pubmed
Shen-Ong,
Two modes of c-myb activation in virus-induced mouse myeloid tumors.
1986,
Pubmed
Shih,
Nucleotide sequence 5' of the chicken c-myc coding region: localization of a noncoding exon that is absent from myc transcripts in most avian leukosis virus-induced lymphomas.
1984,
Pubmed
Stoye,
Role of endogenous retroviruses as mutagens: the hairless mutation of mice.
1988,
Pubmed
Stuhlmann,
Infectivity and methylation of retroviral genomes is correlated with expression in the animal.
1981,
Pubmed
Thompson,
Mapping of DNase I-hypersensitive sites in the 5' and 3' long terminal repeats of integrated moloney murine leukemia virus proviral DNA.
1985,
Pubmed
van der Putten,
Moloney murine leukemia virus-induced tumors: recombinant proviruses in active chromatin regions.
1982,
Pubmed
Varmus,
Retroviruses.
1988,
Pubmed
Zinn,
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
1983,
Pubmed
