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RTA, a candidate G protein-coupled receptor: cloning, sequencing, and tissue distribution.
Ross PC
,
Figler RA
,
Corjay MH
,
Barber CM
,
Adam N
,
Harcus DR
,
Lynch KR
.
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Genomic and cDNA clones, encoding a protein that is a member of the guanine nucleotide-binding regulatory protein (G protein)-coupled receptor superfamily, were isolated by screening rat genomic and thoracic aorta cDNA libraries with an oligonucleotide encoding a highly conserved region of the M1 muscarinic acetylcholine receptor. Sequence analyses of these clones showed that they encode a 343-amino acid protein (named RTA). The RTA gene is single copy, as demonstrated by restriction mapping and Southern blotting of genomic clones and rat genomic DNA. Sequence analysis of the genomic clone further showed that the RTA gene has an intron interrupting the region encoding the amino terminus of the protein. RTA RNA sequences are relatively abundant throughout the gut, vas deferens, uterus, and aorta but are only barely detectable (on Northern blots) in liver, kidney, lung, and salivary gland. In the rat brain, RTA sequences are markedly abundant in the cerebellum. RTA is most closely related to the mas oncogene (34% identity), which has been suggested to be a forebrain angiotensin receptor. We cannot detect angiotensin binding to the RTA protein after introducing the cognate cDNA or mRNA into COS cells or Xenopus oocytes, respectively, nor can we detect an electrophysiologic response in the oocyte after application of angiotensin peptides. We conclude that RTA is not an angiotensin receptor; to date, we have been unable to identify its ligand.
Aruffo,
Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.
1987, Pubmed
Aruffo,
Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.
1987,
Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Bonner,
Identification of a family of muscarinic acetylcholine receptor genes.
1987,
Pubmed
Bonner,
Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes.
1988,
Pubmed
Bunzow,
Cloning and expression of a rat D2 dopamine receptor cDNA.
,
Pubmed
Burnham,
Molecular cloning of rat renin cDNA and its gene.
1987,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Cotecchia,
Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.
1988,
Pubmed
Cross,
Synthesis and expression of functional angiotensin II receptors in Xenopus oocytes injected with rat brain mRNA.
1987,
Pubmed
,
Xenbase
Dixon,
Cloning of the gene and cDNA for mammalian beta-adrenergic receptor and homology with rhodopsin.
,
Pubmed
Dohlman,
The multiple membrane spanning topography of the beta 2-adrenergic receptor. Localization of the sites of binding, glycosylation, and regulatory phosphorylation by limited proteolysis.
1987,
Pubmed
Emorine,
Molecular characterization of the human beta 3-adrenergic receptor.
1989,
Pubmed
Fargin,
The genomic clone G-21 which resembles a beta-adrenergic receptor sequence encodes the 5-HT1A receptor.
1988,
Pubmed
Frielle,
Cloning of the cDNA for the human beta 1-adrenergic receptor.
1987,
Pubmed
,
Xenbase
Gluzman,
SV40-transformed simian cells support the replication of early SV40 mutants.
1981,
Pubmed
Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed
Hayward,
Projected structure of purple membrane determined to 3.7 A resolution by low temperature electron microscopy.
1981,
Pubmed
Healy,
Localization of central angiotensin II receptors with [125I]-sar1, ile8-angiotensin II: periventricular sites of the anterior third ventricle.
1986,
Pubmed
Jackson,
The mas oncogene encodes an angiotensin receptor.
1988,
Pubmed
,
Xenbase
Julius,
Molecular characterization of a functional cDNA encoding the serotonin 1c receptor.
1988,
Pubmed
,
Xenbase
Kobilka,
An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins.
,
Pubmed
Kobilka,
Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor.
1987,
Pubmed
,
Xenbase
Kozak,
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
1986,
Pubmed
Kubo,
Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor.
,
Pubmed
,
Xenbase
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Lipman,
Rapid and sensitive protein similarity searches.
1985,
Pubmed
Loosfelt,
Cloning and sequencing of porcine LH-hCG receptor cDNA: variants lacking transmembrane domain.
1989,
Pubmed
Lynch,
Localization of preangiotensinogen messenger RNA sequences in the rat brain.
1986,
Pubmed
Masu,
cDNA cloning of bovine substance-K receptor through oocyte expression system.
,
Pubmed
,
Xenbase
McFarland,
Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family.
1989,
Pubmed
Mendelsohn,
Autoradiographic localization of angiotensin II receptors in rat brain.
1984,
Pubmed
Meyerhof,
Receptors for neuropeptides are induced by exogenous poly(A)+ RNA in oocytes from Xenopus laevis.
1988,
Pubmed
,
Xenbase
Nathans,
Isolation, sequence analysis, and intron-exon arrangement of the gene encoding bovine rhodopsin.
1983,
Pubmed
Peralta,
Primary structure and biochemical properties of an M2 muscarinic receptor.
1987,
Pubmed
Pritchett,
Structure and functional expression of cloned rat serotonin 5HT-2 receptor.
1988,
Pubmed
,
Xenbase
Regan,
Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.
1988,
Pubmed
Rubenstein,
The hydrophobic tryptic core of the beta-adrenergic receptor retains Gs regulatory activity in response to agonists and thiols.
1987,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sargent,
The rat serum albumin gene: analysis of cloned sequences.
1979,
Pubmed
Schwarzbauer,
Three different fibronectin mRNAs arise by alternative splicing within the coding region.
1983,
Pubmed
Strader,
Identification of residues required for ligand binding to the beta-adrenergic receptor.
1987,
Pubmed
Strader,
Conserved aspartic acid residues 79 and 113 of the beta-adrenergic receptor have different roles in receptor function.
1988,
Pubmed
Yokota,
Molecular characterization of a functional cDNA for rat substance P receptor.
1989,
Pubmed
,
Xenbase
Young,
Isolation and characterization of a new cellular oncogene encoding a protein with multiple potential transmembrane domains.
1986,
Pubmed
Young,
Characterization of the rat mas oncogene and its high-level expression in the hippocampus and cerebral cortex of rat brain.
1988,
Pubmed
