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The binding of the U1 small nuclear ribonucleoprotein (snRNP)-specific proteins C, A, and 70K to U1 small nuclear RNA (snRNA) was analyzed. Assembly of U1 snRNAs from bean and soybean and a set of mutant Xenopus U1 snRNAs into U1 snRNPs in Xenopus egg extracts was studied. The ability to bind proteins was analyzed by immunoprecipitation with monospecific antibodies and by a protein-sequestering assay. The only sequence essential for binding of the U1-specific proteins was the conserved loop sequence in the 5' hairpin of U1. Further analysis suggested that protein C binds directly to the loop and that the assembly of proteins A and 70K into the RNP requires mainly protein-protein interactions. Protein C apparently recognizes a specific RNA sequence rather than a secondary structural element in the RNA.
Adam,
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
1986, Pubmed
Adam,
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
1986,
Pubmed
Billings,
Anti-RNP monoclonal antibodies derived from a mouse strain with lupus-like autoimmunity.
1982,
Pubmed
Branlant,
U2 RNA shares a structural domain with U1, U4, and U5 RNAs.
1982,
Pubmed
Fritz,
Small nuclear U-ribonucleoproteins in Xenopus laevis development. Uncoupled accumulation of the protein and RNA components.
1984,
Pubmed
,
Xenbase
Habets,
Analysis of a cDNA clone expressing a human autoimmune antigen: full-length sequence of the U2 small nuclear RNA-associated B" antigen.
1987,
Pubmed
Hamm,
In vitro assembly of U1 snRNPs.
1987,
Pubmed
,
Xenbase
Huber,
Identification of the binding site on 5S rRNA for the transcription factor IIIA: proposed structure of a common binding site on 5S rRNA and on the gene.
1986,
Pubmed
,
Xenbase
Konarska,
Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes.
1987,
Pubmed
Kramer,
The gapped duplex DNA approach to oligonucleotide-directed mutation construction.
1984,
Pubmed
Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase
Mattaj,
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
1986,
Pubmed
,
Xenbase
Patton,
Reconstitution of the U1 small nuclear ribonucleoprotein particle.
1987,
Pubmed
Patton,
The Mr 70,000 protein of the U1 small nuclear ribonucleoprotein particle binds to the 5' stem-loop of U1 RNA and interacts with Sm domain proteins.
1988,
Pubmed
Sillekens,
cDNA cloning of the human U1 snRNA-associated A protein: extensive homology between U1 and U2 snRNP-specific proteins.
1987,
Pubmed
Spritz,
The human U1-70K snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing and RNA-binding.
1987,
Pubmed
Stefano,
Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts.
1984,
Pubmed
Theissen,
Cloning of the human cDNA for the U1 RNA-associated 70K protein.
1986,
Pubmed
van Santen,
Nucleotide sequence of a bean (Phaseolus vulgaris) U1 small nuclear RNA gene: implications for plant pre-mRNA splicing.
1987,
Pubmed
Wieben,
cDNA cloning of a human autoimmune nuclear ribonucleoprotein antigen.
1985,
Pubmed
Wolin,
The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs.
1984,
Pubmed
Yamamoto,
Isolation and characterization of a complementary DNA expressing human U1 small nuclear ribonucleoprotein C polypeptide.
1988,
Pubmed
Zeller,
Xenopus laevis U1 snRNA genes: characterisation of transcriptionally active genes reveals major and minor repeated gene families.
1984,
Pubmed
,
Xenbase
Zhuang,
A compensatory base change in U1 snRNA suppresses a 5' splice site mutation.
1986,
Pubmed
