Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-28056
Biochem Biophys Res Commun 1987 Jul 15;1461:61-6.
Show Gene links Show Anatomy links

Selective inhibition of DNA polymerase alpha by a polysaccharide purified from slime of Physarum polycephalum.

Shioda M , Murakami-Murofushi K .


???displayArticle.abstract???
A polysaccharide was purified from the slime of a myxomycete, Physarum polycephalum, and its inhibitory effect on eukaryotic DNA polymerases was examined. Almost all the calf thymus DNA polymerase alpha activity was inhibited with higher than 0.2 mg/ml of the polysaccharide, when the assay was carried out with activated DNA as a template. The inhibitory effect occurred regardless of the amounts of the enzyme and deoxyribonucleotides, however, kinetic analysis revealed that the inhibition occurs competitively with the template DNA, the Ki value being 4 micrograms/ml. Inhibition was observed for DNA polymerase alpha, but not for DNA polymerases beta and gamma from various eukaryote species.

???displayArticle.pubmedLink??? 3606625
???displayArticle.link??? Biochem Biophys Res Commun


Species referenced: Xenopus
Genes referenced: pola1